##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544991_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	304507
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84122532486938	34.0	33.0	34.0	31.0	34.0
2	33.19507269126818	34.0	33.0	34.0	31.0	34.0
3	33.32351965636258	34.0	34.0	34.0	31.0	34.0
4	36.57081774803206	37.0	37.0	37.0	35.0	37.0
5	36.56336964339079	37.0	37.0	37.0	35.0	37.0
6	36.35374884649614	37.0	37.0	37.0	35.0	37.0
7	36.15798323191257	37.0	37.0	37.0	35.0	37.0
8	36.316918822884205	37.0	37.0	37.0	35.0	37.0
9	38.184859461358855	39.0	39.0	39.0	37.0	39.0
10	38.11051962680661	39.0	39.0	39.0	35.0	39.0
11	37.49881940316643	39.0	35.0	39.0	35.0	39.0
12	37.44801597336022	39.0	35.0	39.0	35.0	39.0
13	37.437674667577426	39.0	35.0	39.0	35.0	39.0
14	38.6966178117416	41.0	35.0	41.0	35.0	41.0
15	38.75346051158102	41.0	35.0	41.0	35.0	41.0
16	38.80552171214455	41.0	35.0	41.0	35.0	41.0
17	38.89082681186311	41.0	36.0	41.0	35.0	41.0
18	38.866827363574565	41.0	36.0	41.0	35.0	41.0
19	38.819908901930006	41.0	36.0	41.0	35.0	41.0
20	38.727562913167844	41.0	35.0	41.0	35.0	41.0
21	38.676355551760714	41.0	35.0	41.0	35.0	41.0
22	38.64322330849537	41.0	35.0	41.0	35.0	41.0
23	38.60966414565183	41.0	35.0	41.0	35.0	41.0
24	38.591355863740404	41.0	35.0	41.0	35.0	41.0
25	38.55300863362747	40.0	35.0	41.0	35.0	41.0
26	38.5142443359266	40.0	35.0	41.0	35.0	41.0
27	38.57241377045519	41.0	36.0	41.0	35.0	41.0
28	38.5922918028157	41.0	36.0	41.0	35.0	41.0
29	38.59692552223758	41.0	36.0	41.0	35.0	41.0
30	38.54639794815883	41.0	35.0	41.0	35.0	41.0
31	38.47970654204994	41.0	35.0	41.0	35.0	41.0
32	38.41336652359387	41.0	35.0	41.0	35.0	41.0
33	38.342639085472584	40.0	35.0	41.0	35.0	41.0
34	38.26226983287741	40.0	35.0	41.0	35.0	41.0
35	38.20240257202626	40.0	35.0	41.0	35.0	41.0
36	38.14219049151579	40.0	35.0	41.0	35.0	41.0
37	38.0968286443333	40.0	35.0	41.0	35.0	41.0
38	38.07271097216156	40.0	35.0	41.0	35.0	41.0
39	38.01993057630859	40.0	35.0	41.0	35.0	41.0
40	37.97822053351811	40.0	35.0	41.0	35.0	41.0
41	37.96284814470603	40.0	35.0	41.0	35.0	41.0
42	37.888662658001294	40.0	35.0	41.0	35.0	41.0
43	37.82099590485605	40.0	35.0	41.0	35.0	41.0
44	37.68465092756488	40.0	35.0	41.0	35.0	41.0
45	37.69776392660924	40.0	35.0	41.0	35.0	41.0
46	37.6448784428601	40.0	35.0	41.0	35.0	41.0
47	37.56503791374254	40.0	35.0	41.0	35.0	41.0
48	37.53381367259209	39.0	35.0	41.0	35.0	41.0
49	37.478964358783216	39.0	35.0	41.0	35.0	41.0
50	37.38689750974526	39.0	35.0	41.0	35.0	41.0
51	37.316281727513655	39.0	35.0	41.0	35.0	41.0
52	36.889243925427	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	7.0
16	11.0
17	11.0
18	19.0
19	46.0
20	58.0
21	98.0
22	178.0
23	323.0
24	592.0
25	1023.0
26	1415.0
27	1600.0
28	1499.0
29	1478.0
30	1606.0
31	2071.0
32	2717.0
33	4161.0
34	12960.0
35	58841.0
36	9249.0
37	14618.0
38	28608.0
39	159512.0
40	1800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.1648008091768	22.119031746396633	23.965623121964356	22.75054432246221
2	26.333713182291373	24.0138978742689	27.01579930839028	22.63658963504944
3	26.73600278482925	24.20371288673167	26.520900997349816	22.53938333108927
4	26.21745969714982	26.426978690145052	24.38893030373686	22.966631308968267
5	23.00505407100658	34.869477548956176	21.499013158975	20.626455221062244
6	83.29332330619657	2.595999435152558	9.95839176110894	4.1522854975419285
7	82.82305497082169	2.5950142361259347	7.6320084595756414	6.949922333476735
8	70.87062037982706	4.857031201253173	15.465982719609073	8.806365699310689
9	46.259363495748865	17.79203762146683	18.145724072024617	17.802874810759686
10	38.49369636822799	19.350294081909446	28.973718173966446	13.18229137589612
11	36.80605043562217	16.403892192954515	26.247672467299605	20.542384904123715
12	18.822555803314867	31.330314245649525	31.928001655134363	17.919128295901242
13	13.14977980801755	34.67178094428043	31.03935213312009	21.13908711458193
14	11.678549261593329	23.499295582695964	45.12014502129672	19.702010134413985
15	12.339946208133146	17.25871654838805	48.326311053604684	22.075026189874126
16	13.284095275313867	22.435609033618277	30.529675836680276	33.75061985438758
17	15.94840184297898	23.16432791364402	35.88291894767608	25.00435129570092
18	21.08884196422414	28.30181243780931	29.853829304416646	20.755516293549903
19	23.734101350707864	22.090460974624555	27.253232273806518	26.922205400861067
20	16.88631131632442	31.861008121323977	28.595073348067533	22.657607214284074
21	18.197611220760113	23.932454754734703	26.84470307743336	31.025230947071826
22	16.269905125333736	30.79173877776209	23.649702634093796	29.288653462810377
23	16.12146847198915	22.702269570157664	38.31176294797821	22.86449900987498
24	18.130946086625265	22.24053962634685	34.03041637794862	25.598097909079264
25	14.488993684874242	29.061400887335925	30.667603700407543	25.78200172738229
26	16.75166744935256	27.716932615670576	27.183283142916252	28.34811679206061
27	19.256700174380228	25.581021125951125	34.30134611027004	20.8609325893986
28	14.895224083518604	24.194189296140976	31.21767315693892	29.6929134634015
29	17.963462252099294	26.391839924862154	27.026636497683143	28.61806132535541
30	23.623759059726048	23.681229002945745	21.471755985905087	31.223255951423116
31	25.256562246516502	22.63527603634728	24.894665804070186	27.213495913066037
32	20.510201735920685	26.776724344596346	21.854670007586034	30.858403911896936
33	14.708364668135706	23.326228953685792	26.786904734538126	35.178501643640374
34	19.045210783331747	23.704873779584705	28.269957669281826	28.97995776780172
35	22.350881917328667	22.191936474366763	25.749818559179268	29.707363049125306
36	20.853379396861154	29.584213170797387	23.850354835849423	25.712052596492036
37	20.079998160961818	24.90221899660763	26.26179365334787	28.75598918908268
38	19.05473437392244	27.55273277790001	24.001090286922796	29.39144256125475
39	23.851996834227126	23.61883306459293	25.156728745152	27.372441356027938
40	15.140538641147822	21.70820375229469	29.890281668401713	33.260975938155774
41	20.802805846827823	23.604711878544666	24.14263054708102	31.44985172754649
42	20.105613335654024	21.14237111133734	28.999661748334194	29.752353804674442
43	22.602436068793164	26.036183076251117	27.249619877375565	24.111760977580154
44	16.488619309244122	28.48177546000585	26.951432971984225	28.07817225876581
45	20.75485949419882	24.464133829435777	26.250628064379473	28.53037861198593
46	23.55906432364445	20.83071981924882	27.77998535337447	27.830230503732263
47	19.551603083016154	19.164748265228717	32.05082313378674	29.232825517968386
48	19.435349597874595	18.12897568857202	27.40790852098638	35.027766192567
49	20.15947088244277	21.487190770655516	31.265947909243465	27.08739043765825
50	19.22254660812395	22.087176977869145	28.696220448134195	29.994055965872708
51	18.375932244578944	19.46260677094451	30.802247567379403	31.35921341709714
52	19.252102578922653	17.828818385127434	36.295060540480186	26.624018495469727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	94.0
2	142.0
3	725.5
4	1309.0
5	882.0
6	455.0
7	538.5
8	622.0
9	629.5
10	637.0
11	640.5
12	644.0
13	641.5
14	577.5
15	516.0
16	479.5
17	443.0
18	463.0
19	483.0
20	518.5
21	554.0
22	509.5
23	465.0
24	535.5
25	606.0
26	767.5
27	929.0
28	1207.5
29	1486.0
30	1676.0
31	1866.0
32	2110.0
33	2354.0
34	2856.0
35	3358.0
36	4194.5
37	5031.0
38	5344.0
39	6526.5
40	7396.0
41	10336.0
42	13276.0
43	17087.0
44	20898.0
45	23019.5
46	25141.0
47	27356.5
48	29572.0
49	35085.5
50	40599.0
51	41836.0
52	43073.0
53	37197.0
54	31321.0
55	25526.0
56	19731.0
57	16165.5
58	12600.0
59	10931.5
60	9263.0
61	8325.0
62	7387.0
63	6966.5
64	5068.5
65	3591.0
66	2788.0
67	1985.0
68	1683.0
69	1381.0
70	1185.0
71	989.0
72	809.5
73	630.0
74	532.5
75	435.0
76	320.5
77	206.0
78	193.5
79	181.0
80	103.5
81	26.0
82	25.5
83	25.0
84	13.0
85	1.0
86	5.5
87	10.0
88	6.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	304507.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.9201655147659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.15750846688022	23.458341337854012
2	11.944179176001773	8.102970691760007
3	4.280274509901187	4.355628594734453
4	2.070152724684953	2.8087969224862888
5	1.3928984091382728	2.362367229161216
6	1.1442566728067616	2.3288025439788407
7	0.950550241898583	2.256997507471049
8	0.8986292683761319	2.43853228157851
9	0.8966322965688315	2.737252431494948
>10	7.052242644995411	38.90745905135917
>50	0.14677611054432224	3.315387773504655
>100	0.05691318572126781	3.5785460429921527
>500	0.004992384712391913	1.0485688575718064
>1k	0.00399390776991353	2.3003487340528777
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	2325	0.7635292456331053	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	1993	0.6545005533534533	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	1435	0.47125353440150797	No Hit
TGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTTG	1039	0.3412072628872243	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	778	0.2554949475709918	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	623	0.20459299786211813	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	618	0.2029509994844125	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	572	0.18784461440952097	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	505	0.1658418361482659	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	500	0.1641998377705603	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	463	0.15204904977553882	No Hit
CTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTTGAAA	400	0.1313598702164482	Illumina Single End Adapter 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	395	0.12971787183874264	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	338	0.11099909033289874	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6633673445930636	0.0
2	0.0	0.0	0.0	2.2896025378726925	0.0
3	0.0	0.0	0.0	3.4971281448373928	0.0
4	0.0	0.0	0.0	4.262627788523745	0.0
5	0.0	0.0	0.0	8.211962286581262	0.0
6	0.0	0.0	0.0	8.90619920067519	0.0
7	0.0	0.0	0.0	12.531074819298079	0.0
8	0.0	0.0	0.0	16.302745092887847	0.0
9	0.0	0.0	0.0	19.9456826936655	0.0
10	0.0	0.0	0.0	21.53119632717803	0.0
11	0.0	0.0	0.0	23.071390805465885	0.0
12	0.0	0.0	0.0	24.45329664014292	0.0
13	0.0	0.0	0.0	24.943597355725814	0.0
14	0.0	0.0	0.0	25.315674188113903	0.0
15	0.0	0.0	0.0	26.56326455549462	0.0
16	0.0	0.0	0.0	27.886715247925334	0.0
17	0.0	0.0	0.0	29.753010604025523	0.0
18	0.0	0.0	0.0	30.547409419159493	0.0
19	0.0	0.0	0.0	31.498126479851038	0.0
20	0.0	0.0	0.0	32.657377334511196	0.0
21	0.0	0.0	0.0	33.61893158449559	0.0
22	0.0	0.0	0.0	34.7578216592722	0.0
23	0.0	0.0	0.0	35.49048133540444	0.0
24	0.0	0.0	0.0	36.128561904980835	0.0
25	0.0	0.0	0.0	36.791929249573904	0.0
26	0.0	0.0	0.0	37.355791492478005	0.0
27	0.0	0.0	0.0	37.91013014479142	0.0
28	0.0	0.0	0.0	38.47070839094011	0.0
29	0.0	0.0	0.0	39.228654842089014	0.0
30	3.2839967554112054E-4	0.0	0.0	39.92519055391173	0.0
31	3.2839967554112054E-4	0.0	0.0	40.53634235009376	0.0
32	3.2839967554112054E-4	0.0	0.0	41.103816989428815	0.0
33	3.2839967554112054E-4	0.0	0.0	41.616448882948504	0.0
34	3.2839967554112054E-4	0.0	0.0	42.340570167516674	0.0
35	3.2839967554112054E-4	0.0	0.0	42.86141205292489	0.0
36	3.2839967554112054E-4	0.0	0.0	43.42494589615345	0.0
37	3.2839967554112054E-4	0.0	0.0	43.94053338675301	0.0
38	3.2839967554112054E-4	0.0	0.0	44.47615325756058	0.0
39	6.567993510822411E-4	0.0	0.0	45.02556591474087	0.0
40	6.567993510822411E-4	0.0	0.0	45.61996932747031	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGT	55	1.8189894E-12	46.000004	40
CGTGAGT	55	1.8189894E-12	46.000004	31
TCACGAC	20	6.3041114E-4	46.0	24
ATGATAG	20	6.3041114E-4	46.0	19
ATAGGGT	20	6.3041114E-4	46.0	3
CGACGGT	20	6.3041114E-4	46.0	27
CATGCGG	40	5.5879354E-9	46.0	1
GCGCGAC	25	3.4112003E-5	46.0	8
TTTCGGG	25	3.4112003E-5	46.0	2
ATAATGG	20	6.3041114E-4	46.0	1
GTCTCAC	20	6.3041114E-4	46.0	21
CCTACTA	20	6.3041114E-4	46.0	17
CACTGTT	20	6.3041114E-4	46.0	20
CGCGACC	25	3.4112003E-5	46.0	9
ACTTAGG	25	3.4112003E-5	46.0	1
ACGGGCG	20	6.3041114E-4	46.0	4
ACGGGAA	95	0.0	46.0	4
TAGTTGG	20	6.3041114E-4	46.0	1
AATGCGG	50	1.6370905E-11	46.0	1
CTAACGG	25	3.4112003E-5	46.0	1
>>END_MODULE