FastQCFastQC Report
Thu 26 May 2016
SRR1544990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544990_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences774667
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT26240.33872618815568495Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT22220.28683292304951674Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT18870.24358853546104325Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.15826154980139853No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC10090.13024951366199927No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT9500.12263333793746216Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT206.310173E-446.00000419
AGCAACT206.310173E-446.00000410
GTCGCAA206.310173E-446.00000420
ACACGTA206.310173E-446.00000445
CTCAATC206.310173E-446.00000426
CTCGTCA206.310173E-446.00000446
TCCGAAT206.310173E-446.00000438
ACCGCTT206.310173E-446.00000436
CCAATCC206.310173E-446.00000421
CGATTGA206.310173E-446.0000049
TACGGGT800.046.0000043
ATCGTAA206.310173E-446.00000422
TAGCAAC206.310173E-446.0000049
CGCTCGA206.310173E-446.00000414
GTTAACG206.310173E-446.00000426
AGGCACG206.310173E-446.00000439
GCTTACG206.310173E-446.00000426
GACGGAC206.310173E-446.0000048
GAACGAT206.310173E-446.00000439
GGACGGA206.310173E-446.0000047
Produced by FastQC (version 0.11.5)