##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544990_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	774667
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94298453399977	34.0	33.0	34.0	31.0	34.0
2	33.24528991166527	34.0	33.0	34.0	31.0	34.0
3	33.29105667338353	34.0	34.0	34.0	31.0	34.0
4	36.567843989740105	37.0	37.0	37.0	35.0	37.0
5	36.5643741117151	37.0	37.0	37.0	35.0	37.0
6	36.67168860942831	37.0	37.0	37.0	35.0	37.0
7	36.52755442015731	37.0	37.0	37.0	35.0	37.0
8	36.5447527776451	37.0	37.0	37.0	35.0	37.0
9	38.32385011882525	39.0	39.0	39.0	37.0	39.0
10	38.103320523528176	39.0	39.0	39.0	35.0	39.0
11	37.202311444788535	39.0	35.0	39.0	35.0	39.0
12	37.07009463421057	39.0	35.0	39.0	35.0	39.0
13	37.04829429935701	39.0	35.0	39.0	35.0	39.0
14	38.25834971671699	40.0	35.0	41.0	35.0	41.0
15	38.33657042316247	40.0	35.0	41.0	35.0	41.0
16	38.35989141140645	40.0	35.0	41.0	35.0	41.0
17	38.4409236484838	40.0	36.0	41.0	35.0	41.0
18	38.435682686883524	40.0	36.0	41.0	35.0	41.0
19	38.33456310905202	40.0	35.0	41.0	35.0	41.0
20	38.23776796997936	40.0	35.0	41.0	35.0	41.0
21	38.190502499783776	40.0	35.0	41.0	35.0	41.0
22	38.17084243939654	40.0	35.0	41.0	35.0	41.0
23	38.1627434755837	40.0	35.0	41.0	35.0	41.0
24	38.12096294278703	40.0	35.0	41.0	35.0	41.0
25	38.0896798237178	40.0	35.0	41.0	35.0	41.0
26	38.04983689766054	40.0	35.0	41.0	35.0	41.0
27	38.056322264921576	40.0	35.0	41.0	35.0	41.0
28	38.09556235130708	40.0	35.0	41.0	35.0	41.0
29	38.113342894430765	40.0	35.0	41.0	35.0	41.0
30	38.019159200017555	40.0	35.0	41.0	35.0	41.0
31	37.94637695939029	40.0	35.0	41.0	35.0	41.0
32	37.87338043314095	40.0	35.0	41.0	35.0	41.0
33	37.79795189416872	40.0	35.0	41.0	35.0	41.0
34	37.714030673825015	40.0	35.0	41.0	35.0	41.0
35	37.64759309483946	40.0	35.0	41.0	35.0	41.0
36	37.58092186707321	40.0	35.0	41.0	35.0	41.0
37	37.53691457103504	40.0	35.0	41.0	35.0	41.0
38	37.48825882605042	39.0	35.0	41.0	35.0	41.0
39	37.38454587584084	39.0	35.0	41.0	35.0	41.0
40	37.35015948788318	39.0	35.0	41.0	34.0	41.0
41	37.33456310905202	39.0	35.0	41.0	34.0	41.0
42	37.2332189185805	39.0	35.0	41.0	34.0	41.0
43	37.15092420356101	38.0	35.0	41.0	34.0	41.0
44	37.0916045216848	38.0	35.0	41.0	34.0	41.0
45	37.06202923320601	38.0	35.0	41.0	34.0	41.0
46	37.080315800208346	38.0	35.0	41.0	34.0	41.0
47	37.04238466334567	38.0	35.0	41.0	34.0	41.0
48	37.057835173048545	38.0	35.0	41.0	34.0	41.0
49	36.9983089508137	38.0	35.0	41.0	34.0	41.0
50	36.91907490573369	38.0	35.0	41.0	34.0	41.0
51	36.85751942447529	37.0	35.0	41.0	34.0	41.0
52	36.683550480399965	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	0.0
13	1.0
14	12.0
15	12.0
16	13.0
17	38.0
18	61.0
19	116.0
20	210.0
21	328.0
22	536.0
23	860.0
24	1519.0
25	2523.0
26	3436.0
27	3947.0
28	4019.0
29	4436.0
30	5049.0
31	6271.0
32	8346.0
33	12565.0
34	41405.0
35	197721.0
36	24552.0
37	37743.0
38	66416.0
39	349659.0
40	2870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.666122346763192	22.800893803401976	25.45881004354129	20.074173806293544
2	26.802871427335873	23.828561175317912	27.148052001698797	22.220515395647418
3	25.81883570618085	25.687295315277403	26.056486206331236	22.437382772210512
4	25.987424273913824	27.060143261556256	23.639834922618363	23.312597541911558
5	23.255540767839598	37.21560360774372	20.635189055426395	18.893666568990287
6	87.72995364459825	2.8173395794580123	6.810410150425924	2.642296625517803
7	88.0255645328896	2.141694431284668	5.159249070891105	4.673491964934611
8	77.58533666723896	4.551762241066162	11.330545899076636	6.5323551926182475
9	53.99468416751972	15.32310011914797	15.934717756145545	14.747497957186765
10	47.229067457372004	18.420301884551684	22.19805413164624	12.152576526430066
11	43.35462850489307	16.328048051614434	23.719869311588077	16.597454131904417
12	20.416772626173568	36.924510789797424	26.684497984295184	15.974218599733822
13	13.20799775903711	40.470808747500534	28.485529911562	17.835663581900352
14	11.812946724205368	21.505240316161654	49.323773957068006	17.358039002564972
15	13.27099256841972	16.561825920040484	50.39739655877945	19.769784952760347
16	14.15124175936241	18.755026353258884	28.66754360260602	38.426188284772685
17	18.615224348010177	21.674603410239495	36.35510483859517	23.35506740315516
18	25.02352623772537	25.514963203544234	27.73862833966078	21.722882219069614
19	27.90889504780764	21.758897694105983	25.998396730466123	24.333810527620255
20	19.422926237983546	31.330236088538687	27.35756137798564	21.889276295492127
21	20.463889645486383	24.068793429951192	25.059799888210033	30.407517036352395
22	16.81677417522626	31.648179153107076	22.280541187374705	29.25450548429196
23	17.437298865189817	22.671160640636558	38.50777172643213	21.383768767741493
24	16.76823719094785	21.770128326106573	37.465259266239556	23.996375216706017
25	14.948358455955916	30.71732757429967	30.588110762430826	23.74620320731359
26	17.101928957861894	30.94348926700118	27.659239389311796	24.295342385825137
27	17.42581005774094	27.72197602324612	37.71698032832172	17.135233590691225
28	15.003866177338136	24.996417815654983	33.055493521732565	26.944222485274317
29	17.339966721184716	29.67920409672801	29.83359301480507	23.147236167282202
30	26.453560045800323	27.90450606518672	21.860102469835425	23.78183141917753
31	27.79323244697399	23.982562830222534	25.118922065868304	23.105282656935174
32	24.734756998813683	29.888971648463148	24.183552416715827	21.192718936007342
33	17.356102686702805	28.979032280967175	27.20097796859812	26.4638870637319
34	16.221679766919205	27.45114997799054	28.5536882299104	27.773482025179852
35	29.402956367058366	24.68492913729383	25.014231921586955	20.897882574060855
36	19.308812689839634	31.077740500111663	27.87675220449561	21.736694605553094
37	20.782478148675494	30.84112270175443	23.751108540831094	24.625290608738982
38	19.676067265031296	28.205538637892154	26.39056523641771	25.727828860658835
39	25.518706747544428	23.478217091989205	27.343748991502153	23.659327168964214
40	15.902058561936935	20.908338679716575	32.0320860447134	31.157516713633083
41	21.42985308526115	24.19413761009569	25.77326773955777	28.60274156508539
42	21.657434742928253	22.753518608640874	27.941683329740393	27.64736331869048
43	21.2619099561489	27.54551310434032	28.233808849479843	22.958768090030944
44	15.240225800247073	34.19082005558518	25.62868948851571	24.94026465565204
45	22.594095269322175	29.17008211270133	25.48927474643944	22.74654787153706
46	22.519353477042394	24.416813934245297	27.28101235756783	25.782820231144477
47	22.456745930832213	20.8858774157154	31.4455114261999	25.211865227252485
48	19.70743558199846	19.037341205963337	30.908893756930393	30.346329455107806
49	20.144784791400692	25.866469076390242	29.430193876852893	24.558552255356172
50	18.888373972300357	27.796846903250046	26.910530589272554	26.40424853517705
51	19.380069113567508	21.57081688002716	31.63578673158919	27.413327274816147
52	22.5849300409079	19.92985373069977	33.8067840762547	23.67843215213763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	250.0
2	334.0
3	1381.5
4	2429.0
5	1727.0
6	1025.0
7	1112.5
8	1200.0
9	1237.5
10	1275.0
11	1265.5
12	1256.0
13	1172.5
14	1103.5
15	1118.0
16	956.0
17	794.0
18	940.5
19	1087.0
20	1048.5
21	1010.0
22	1169.5
23	1329.0
24	1714.0
25	2099.0
26	2147.5
27	2196.0
28	3280.5
29	4365.0
30	4681.5
31	4998.0
32	6021.0
33	7044.0
34	8679.5
35	10315.0
36	11775.5
37	13236.0
38	14299.0
39	18056.0
40	20750.0
41	34455.5
42	48161.0
43	70482.0
44	92803.0
45	104700.5
46	116598.0
47	111606.5
48	106615.0
49	91143.5
50	75672.0
51	66123.0
52	56574.0
53	48290.0
54	40006.0
55	35251.5
56	30497.0
57	27957.5
58	25418.0
59	24191.5
60	22965.0
61	23216.0
62	23467.0
63	19292.0
64	12522.5
65	9928.0
66	8201.5
67	6475.0
68	5191.5
69	3908.0
70	3124.5
71	2341.0
72	1975.5
73	1610.0
74	1294.5
75	979.0
76	795.0
77	611.0
78	425.5
79	240.0
80	159.5
81	79.0
82	48.0
83	17.0
84	53.5
85	90.0
86	48.0
87	6.0
88	9.0
89	6.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	774667.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.50803718343596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.36323545903788	15.38722245692012
2	12.763616536880996	5.745679112144711
3	5.093370362180251	3.4392530850289136
4	2.5330206103632054	2.2805328833785867
5	1.5014223724399194	1.6897035293460168
6	0.9630564109624977	1.3005905704614167
7	0.6286395835442384	0.9904610184975391
8	0.4683065576616338	0.843252913047596
9	0.38101728030591064	0.771835600139137
>10	5.262599704632569	31.415576731859073
>50	1.7907459104916355	26.00933798044497
>100	0.24463741946606923	8.416618712851442
>500	0.0034537047454033304	0.548039609552539
>1k	0.0028780872878361088	1.1618957963279362
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	2624	0.33872618815568495	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	2222	0.28683292304951674	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT	1887	0.24358853546104325	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1226	0.15826154980139853	No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	1009	0.13024951366199927	No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	950	0.12263333793746216	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2461702899439372	0.0
2	0.0	0.0	0.0	1.097891093850648	0.0
3	0.0	0.0	0.0	1.8104553311293756	0.0
4	0.0	0.0	0.0	2.2426410315658214	0.0
5	0.0	0.0	0.0	4.768629617629252	0.0
6	0.0	0.0	0.0	5.259033881654957	0.0
7	0.0	0.0	0.0	7.737130922060705	0.0
8	1.29087724144697E-4	0.0	0.0	10.406019618752316	0.0
9	1.29087724144697E-4	0.0	0.0	13.07774824537511	0.0
10	1.29087724144697E-4	0.0	0.0	14.118453477429657	0.0
11	1.29087724144697E-4	0.0	0.0	15.138246498172764	0.0
12	1.29087724144697E-4	0.0	0.0	16.011137688839206	0.0
13	1.29087724144697E-4	0.0	0.0	16.33914959589088	0.0
14	1.29087724144697E-4	0.0	0.0	16.590483394800604	0.0
15	1.29087724144697E-4	0.0	0.0	17.54095630767801	0.0
16	1.29087724144697E-4	0.0	0.0	18.52486294110889	0.0
17	1.29087724144697E-4	0.0	0.0	20.05726331443059	0.0
18	1.29087724144697E-4	0.0	0.0	20.597753615424434	0.0
19	1.29087724144697E-4	0.0	0.0	21.428562208019702	0.0
20	1.29087724144697E-4	0.0	0.0	22.25601451978721	0.0
21	1.29087724144697E-4	0.0	0.0	22.934112334719305	0.0
22	1.29087724144697E-4	0.0	0.0	23.762984611452406	0.0
23	1.29087724144697E-4	0.0	0.0	24.31573824624	0.0
24	1.29087724144697E-4	0.0	0.0	24.78936110612689	0.0
25	1.29087724144697E-4	0.0	0.0	25.295126809325815	0.0
26	1.29087724144697E-4	0.0	0.0	25.729119737900284	0.0
27	1.29087724144697E-4	0.0	0.0	26.18325035144133	0.0
28	1.29087724144697E-4	0.0	0.0	26.614661525532906	0.0
29	1.29087724144697E-4	0.0	0.0	27.215306705978183	0.0
30	1.29087724144697E-4	0.0	0.0	27.80807753525063	0.0
31	1.29087724144697E-4	0.0	0.0	28.264918990998712	0.0
32	1.29087724144697E-4	0.0	0.0	28.734023780540543	0.0
33	1.29087724144697E-4	0.0	0.0	29.162595024700938	0.0
34	1.29087724144697E-4	0.0	0.0	29.823007821425207	0.0
35	2.58175448289394E-4	0.0	0.0	30.257775276344546	0.0
36	2.58175448289394E-4	0.0	0.0	30.72842911857611	0.0
37	2.58175448289394E-4	0.0	0.0	31.148996923839533	0.0
38	2.58175448289394E-4	0.0	0.0	31.551621535446845	0.0
39	2.58175448289394E-4	0.0	0.0	32.03789499229992	0.0
40	2.58175448289394E-4	0.0	0.0	32.502481711496685	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	20	6.310173E-4	46.000004	19
AGCAACT	20	6.310173E-4	46.000004	10
GTCGCAA	20	6.310173E-4	46.000004	20
ACACGTA	20	6.310173E-4	46.000004	45
CTCAATC	20	6.310173E-4	46.000004	26
CTCGTCA	20	6.310173E-4	46.000004	46
TCCGAAT	20	6.310173E-4	46.000004	38
ACCGCTT	20	6.310173E-4	46.000004	36
CCAATCC	20	6.310173E-4	46.000004	21
CGATTGA	20	6.310173E-4	46.000004	9
TACGGGT	80	0.0	46.000004	3
ATCGTAA	20	6.310173E-4	46.000004	22
TAGCAAC	20	6.310173E-4	46.000004	9
CGCTCGA	20	6.310173E-4	46.000004	14
GTTAACG	20	6.310173E-4	46.000004	26
AGGCACG	20	6.310173E-4	46.000004	39
GCTTACG	20	6.310173E-4	46.000004	26
GACGGAC	20	6.310173E-4	46.000004	8
GAACGAT	20	6.310173E-4	46.000004	39
GGACGGA	20	6.310173E-4	46.000004	7
>>END_MODULE