FastQCFastQC Report
Thu 26 May 2016
SRR1544989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544989_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences463959
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT21490.46318747992818327Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT15270.3291239096558101Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT14290.3080013535678799Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTTG9280.20001767397550213Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.17867958160096042No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6670.14376270316989215No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC6380.1375121508581577No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG6150.13255481626609247No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT5920.12759748167402724Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC5200.11207886903799688No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC206.307543E-446.00000445
TCGTTAG206.307543E-446.00000441
CTCGTTA206.307543E-446.00000440
TTCACGG206.307543E-446.0000041
GATCCAC206.307543E-446.00000414
GGTCATC206.307543E-446.0000047
TAAGTAG206.307543E-446.00000414
TATGCGT206.307543E-446.00000419
TCGATAT206.307543E-446.00000415
GGGCGAA351.0177973E-746.0000046
TGTTAGG800.046.0000041
GTCCGAC206.307543E-446.0000048
GGTGCTA206.307543E-446.0000047
AATTAGT206.307543E-446.00000445
ACGTAGG351.0177973E-746.0000041
CACGTGA206.307543E-446.00000442
TTCGATA206.307543E-446.00000414
CGTTTAG206.307543E-446.00000429
ATTTCGG206.307543E-446.0000041
AATCCGG253.4139845E-546.01
Produced by FastQC (version 0.11.5)