##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544989_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	463959
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94665907978938	34.0	33.0	34.0	31.0	34.0
2	33.29017865802797	34.0	34.0	34.0	31.0	34.0
3	33.40487413758543	34.0	34.0	34.0	31.0	34.0
4	36.622852450324274	37.0	37.0	37.0	35.0	37.0
5	36.621313521237866	37.0	37.0	37.0	35.0	37.0
6	36.44240762653597	37.0	37.0	37.0	35.0	37.0
7	36.29153653663362	37.0	37.0	37.0	35.0	37.0
8	36.41160533581631	37.0	37.0	37.0	35.0	37.0
9	38.26894617843387	39.0	39.0	39.0	37.0	39.0
10	38.12674826870478	39.0	39.0	39.0	35.0	39.0
11	37.30767158304936	39.0	35.0	39.0	35.0	39.0
12	37.24528244952679	39.0	35.0	39.0	35.0	39.0
13	37.23905345084372	39.0	35.0	39.0	35.0	39.0
14	38.39524182093676	40.0	35.0	41.0	35.0	41.0
15	38.447703353098014	41.0	35.0	41.0	35.0	41.0
16	38.4946600884992	41.0	35.0	41.0	35.0	41.0
17	38.59375289626885	41.0	36.0	41.0	35.0	41.0
18	38.59683290980453	40.0	36.0	41.0	35.0	41.0
19	38.53063525009753	40.0	35.0	41.0	35.0	41.0
20	38.4486581788477	40.0	35.0	41.0	35.0	41.0
21	38.401272526236156	40.0	35.0	41.0	35.0	41.0
22	38.385036177765706	40.0	35.0	41.0	35.0	41.0
23	38.359587808405486	40.0	35.0	41.0	35.0	41.0
24	38.33164783957203	40.0	35.0	41.0	35.0	41.0
25	38.29890787763574	40.0	35.0	41.0	35.0	41.0
26	38.2456122200453	40.0	35.0	41.0	35.0	41.0
27	38.34173709314832	40.0	35.0	41.0	35.0	41.0
28	38.36742901851241	40.0	35.0	41.0	35.0	41.0
29	38.383085574371876	40.0	36.0	41.0	35.0	41.0
30	38.30228533124694	40.0	35.0	41.0	35.0	41.0
31	38.23345166275468	40.0	35.0	41.0	35.0	41.0
32	38.18361536256437	40.0	35.0	41.0	35.0	41.0
33	38.100401113029385	40.0	35.0	41.0	35.0	41.0
34	38.0014311609431	40.0	35.0	41.0	35.0	41.0
35	37.93489079854039	40.0	35.0	41.0	35.0	41.0
36	37.85005787149296	40.0	35.0	41.0	35.0	41.0
37	37.83557814375839	40.0	35.0	41.0	35.0	41.0
38	37.78203246407549	40.0	35.0	41.0	35.0	41.0
39	37.715836959731355	40.0	35.0	41.0	35.0	41.0
40	37.6951993602883	40.0	35.0	41.0	35.0	41.0
41	37.68139857185656	40.0	35.0	41.0	35.0	41.0
42	37.6108988078688	40.0	35.0	41.0	35.0	41.0
43	37.54819499136777	39.0	35.0	41.0	35.0	41.0
44	37.43976730702497	39.0	35.0	41.0	35.0	41.0
45	37.455701904694166	39.0	35.0	41.0	35.0	41.0
46	37.39912794018437	39.0	35.0	41.0	35.0	41.0
47	37.33547145329652	39.0	35.0	41.0	35.0	41.0
48	37.289415659573365	39.0	35.0	41.0	35.0	41.0
49	37.23717828515019	39.0	35.0	41.0	35.0	41.0
50	37.16978655441537	38.0	35.0	41.0	35.0	41.0
51	37.107041786019884	38.0	35.0	41.0	35.0	41.0
52	36.703124629547005	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	1.0
14	8.0
15	2.0
16	6.0
17	13.0
18	24.0
19	45.0
20	104.0
21	143.0
22	239.0
23	462.0
24	814.0
25	1304.0
26	1850.0
27	2094.0
28	2008.0
29	2064.0
30	2393.0
31	2867.0
32	3948.0
33	6098.0
34	22570.0
35	113118.0
36	13318.0
37	22195.0
38	41043.0
39	223846.0
40	1379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.849493166422032	22.69489329876131	24.547643218474047	21.907970316342606
2	26.557734627413197	24.18812869240601	27.101532678534095	22.152604001646697
3	25.813487829743575	25.77878648759912	25.98570132274619	22.422024359911113
4	26.206841552809625	26.72348203181747	23.699939003230888	23.369737412142022
5	23.191919975687505	36.68082740069705	20.631779963315726	19.495472660299725
6	86.4800553497184	2.7562780331882775	7.573082966382804	3.190583650710516
7	86.66584762877754	2.0894087624121958	5.783054106074028	5.461689502736233
8	75.57111727544891	4.556868171541019	12.629133177716135	7.242881375293937
9	52.90898549225255	15.091204179679671	16.39347442338655	15.60633590468123
10	45.541308607010535	18.166691453339627	23.921294769580932	12.370705170068907
11	41.767483764729214	16.211346261199804	24.31033776691475	17.710832207156237
12	19.861022202392885	35.173151075849376	28.18999092592233	16.77583579583541
13	13.323591093178491	38.552760049918206	29.27090540327917	18.852743453624136
14	11.74112367687662	21.365034410368157	48.63705629161198	18.256785621143248
15	13.133488088387121	16.497147377246694	49.867768488163826	20.50159604620236
16	13.985718565649119	19.521121478406496	29.19374341267224	37.29941654327214
17	18.186305255421274	21.67325130022265	36.60560523666962	23.534838207686455
18	24.145668043943537	26.337456542496213	27.88134296349462	21.635532450065632
19	26.95173495933908	22.020264721667214	26.00811709655379	25.019883222439915
20	18.924948109638997	32.25306546483633	26.869615634140086	21.952370791384585
21	19.787955401231574	24.12777853215478	25.239299162210454	30.844966904403194
22	16.50081149411909	32.2748346297841	22.287529717065517	28.936824159031293
23	17.1064684594975	22.879392360100784	38.12190301298175	21.892236167419966
24	16.96464558290711	22.14850881220108	36.66164467118862	24.225200933703196
25	14.856054090986486	30.521015865626055	30.888936306871944	23.73399373651551
26	17.135565858190056	29.65714642888704	27.752452264100924	25.45483544882199
27	18.03736968137271	27.194428818063663	37.422703299213936	17.345498201349688
28	15.450718705747708	24.800036210096152	32.67573212288155	27.07351296127459
29	17.73648102526301	28.974974081761534	29.54269666069631	23.745848232279148
30	26.10898807868799	27.707620716485724	21.418056336874596	24.765334867951694
31	27.604809907772022	23.64799475815751	24.575016326873712	24.172179007196757
32	24.028631840313473	29.76642332619908	24.360126649122012	21.844818184365426
33	16.765058981504833	28.57084354436491	27.595757383734338	27.06834009039592
34	16.364377024693994	27.45070146284478	28.463506473632368	27.721415038828862
35	28.61265758396755	24.938626042387366	24.831935580514656	21.616780793130427
36	19.05728738961848	31.09822204117174	27.203481342101348	22.64100922710843
37	21.179026595022403	30.7236199750409	23.85598727473764	24.24136615519906
38	19.66359096385672	27.65869397942491	25.891943038070174	26.785772018648203
39	25.98979651219181	23.00634323291498	27.540364558075176	23.463495696818036
40	16.00658678891885	20.36149745990486	32.07955875411405	31.55235699706224
41	21.541774165389615	24.25860905812798	25.38112203880084	28.81849473768156
42	21.465043247355908	22.5582432930496	28.138477753422176	27.838235706172316
43	22.07005360387448	27.15714104047987	28.122743604499533	22.650061751146115
44	15.20931806474279	32.67185246972254	26.68727193566673	25.431557529867945
45	22.33753413555939	28.507906948674343	25.789347765643083	23.36521115012318
46	22.931336605174163	23.149674863511645	27.589722367709214	26.329266163604974
47	22.137085389010664	20.04034839285368	31.771341864259554	26.051224353876094
48	19.46637526160717	18.49064249211676	30.62167131147364	31.421310934802428
49	19.76359980084447	24.80262264553549	30.13111072314579	25.302666830474248
50	19.101903400947066	26.774995204317626	27.620759592981276	26.502341801754035
51	19.35817604572818	20.572723020784164	31.870057483527642	28.19904344996002
52	22.48366773788201	19.500861067465014	34.13836136382741	23.877109830825567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	154.5
2	247.0
3	897.5
4	1548.0
5	1098.0
6	648.0
7	681.5
8	715.0
9	764.0
10	813.0
11	800.5
12	788.0
13	743.5
14	691.0
15	683.0
16	622.0
17	561.0
18	638.0
19	715.0
20	631.0
21	547.0
22	656.5
23	766.0
24	964.0
25	1162.0
26	1176.5
27	1191.0
28	1710.0
29	2229.0
30	2492.5
31	2756.0
32	3279.5
33	3803.0
34	4601.5
35	5400.0
36	6313.5
37	7227.0
38	7947.0
39	10160.0
40	11653.0
41	19271.0
42	26889.0
43	40117.0
44	53345.0
45	62318.5
46	71292.0
47	68348.5
48	65405.0
49	56498.0
50	47591.0
51	41526.0
52	35461.0
53	30042.5
54	24624.0
55	21422.0
56	18220.0
57	16700.0
58	15180.0
59	14409.5
60	13639.0
61	13742.5
62	13846.0
63	11619.0
64	7716.0
65	6040.0
66	5008.0
67	3976.0
68	3194.5
69	2413.0
70	1944.0
71	1475.0
72	1247.5
73	1020.0
74	797.0
75	574.0
76	482.0
77	390.0
78	285.0
79	180.0
80	112.5
81	45.0
82	31.0
83	17.0
84	36.5
85	56.0
86	28.5
87	1.0
88	3.5
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	463959.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.537540969600176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.48390837530795	19.409535145825878
2	11.67444001141421	6.429707402229168
3	4.232548170476739	3.4966190595092845
4	2.0489538087793506	2.2569259781631876
5	1.2327334050739371	1.6973223323409135
6	0.7994259734199751	1.320853529712904
7	0.625448459242663	1.2056318799537697
8	0.5035990197581963	1.1094302911073457
9	0.3990944249412488	0.9891071169802794
>10	7.523769897091399	49.21086112523794
>50	0.3873047492833477	7.18542136313383
>100	0.08091762510382314	3.5481452332377295
>500	0.005499256075017107	1.0360385050896583
>1k	0.0023568240321501887	1.1044010374781177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	2149	0.46318747992818327	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT	1527	0.3291239096558101	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	1429	0.3080013535678799	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTTG	928	0.20001767397550213	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	829	0.17867958160096042	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	667	0.14376270316989215	No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	638	0.1375121508581577	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	615	0.13255481626609247	No Hit
TCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	592	0.12759748167402724	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	520	0.11207886903799688	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3349433893943215	0.0
2	0.0	0.0	0.0	1.3417133841567896	0.0
3	0.0	0.0	0.0	2.1454481969311945	0.0
4	0.0	0.0	0.0	2.6420438012841654	0.0
5	0.0	0.0	0.0	5.566009065456215	0.0
6	0.0	0.0	0.0	6.12230822120058	0.0
7	0.0	0.0	0.0	9.111796516502535	0.0
8	0.0	0.0	0.0	12.103655710957218	0.0
9	0.0	0.0	0.0	15.24466601574708	0.0
10	0.0	0.0	0.0	16.45123814819844	0.0
11	0.0	0.0	0.0	17.604141745283528	0.0
12	0.0	0.0	0.0	18.613282639198722	0.0
13	0.0	0.0	0.0	18.988746850476012	0.0
14	0.0	0.0	0.0	19.26312454333249	0.0
15	0.0	0.0	0.0	20.29791425535446	0.0
16	0.0	0.0	0.0	21.349515797732128	0.0
17	0.0	0.0	0.0	22.901808133908386	0.0
18	0.0	0.0	0.0	23.491515414077536	0.0
19	0.0	0.0	0.0	24.34848768964499	0.0
20	0.0	0.0	0.0	25.19468315088187	0.0
21	0.0	0.0	0.0	25.893451792076455	0.0
22	0.0	0.0	0.0	26.758398910248534	0.0
23	0.0	0.0	0.0	27.350692625857025	0.0
24	0.0	0.0	0.0	27.843193040764376	0.0
25	0.0	0.0	0.0	28.352289749740816	0.0
26	0.0	0.0	0.0	28.812028649083217	0.0
27	0.0	0.0	0.0	29.301511555978006	0.0
28	0.0	0.0	0.0	29.724609286596444	0.0
29	0.0	0.0	0.0	30.325093381096174	0.0
30	0.0	0.0	0.0	30.896049004330123	0.0
31	0.0	0.0	0.0	31.3441489441955	0.0
32	0.0	0.0	0.0	31.81660448444798	0.0
33	0.0	0.0	0.0	32.22461467500361	0.0
34	0.0	0.0	0.0	32.865835127672916	0.0
35	2.1553628661153247E-4	0.0	0.0	33.295183410603094	0.0
36	2.1553628661153247E-4	0.0	0.0	33.772596285447634	0.0
37	2.1553628661153247E-4	0.0	0.0	34.19095221776062	0.0
38	2.1553628661153247E-4	0.0	0.0	34.602195452615426	0.0
39	2.1553628661153247E-4	0.0	0.0	35.07982386374658	0.0
40	2.1553628661153247E-4	0.0	0.0	35.52964809390485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAC	20	6.307543E-4	46.000004	45
TCGTTAG	20	6.307543E-4	46.000004	41
CTCGTTA	20	6.307543E-4	46.000004	40
TTCACGG	20	6.307543E-4	46.000004	1
GATCCAC	20	6.307543E-4	46.000004	14
GGTCATC	20	6.307543E-4	46.000004	7
TAAGTAG	20	6.307543E-4	46.000004	14
TATGCGT	20	6.307543E-4	46.000004	19
TCGATAT	20	6.307543E-4	46.000004	15
GGGCGAA	35	1.0177973E-7	46.000004	6
TGTTAGG	80	0.0	46.000004	1
GTCCGAC	20	6.307543E-4	46.000004	8
GGTGCTA	20	6.307543E-4	46.000004	7
AATTAGT	20	6.307543E-4	46.000004	45
ACGTAGG	35	1.0177973E-7	46.000004	1
CACGTGA	20	6.307543E-4	46.000004	42
TTCGATA	20	6.307543E-4	46.000004	14
CGTTTAG	20	6.307543E-4	46.000004	29
ATTTCGG	20	6.307543E-4	46.000004	1
AATCCGG	25	3.4139845E-5	46.0	1
>>END_MODULE