FastQCFastQC Report
Thu 26 May 2016
SRR1544988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544988_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1040540
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT46560.4474599727064793TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT33440.32137159551771194Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT33070.31781574951467506TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.17788840409787227No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC13800.132623445518673No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT12510.1202260364810579TruSeq Adapter, Index 13 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC11900.11436369577334846TruSeq Adapter, Index 13 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10590.10177407884367731No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG206.311176E-446.00000445
GTACCGT206.311176E-446.00000417
GTACCGG206.311176E-446.0000041
CTCGTTA206.311176E-446.00000444
TTTACGG206.311176E-446.0000041
CGTTAGG206.311176E-446.0000041
ACTAGTG206.311176E-446.00000432
CTAACGG1450.046.0000041
ACGGCTA206.311176E-446.00000414
ACGTGCG206.311176E-446.00000410
CGACTAG206.311176E-446.00000429
GCGTATT206.311176E-446.00000439
TCACGTA206.311176E-446.00000432
ACTATAG301.8612318E-646.032
TAAACGG551.8189894E-1246.01
GTTAGAC253.416931E-546.010
CACGATA301.8612318E-646.018
ACGGAAT453.092282E-1046.04
CGAATAT551.8189894E-1246.014
ACGTAGG1100.046.01
Produced by FastQC (version 0.11.5)