##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544988_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1040540
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.900251792338594	34.0	33.0	34.0	31.0	34.0
2	33.196730543756125	34.0	33.0	34.0	31.0	34.0
3	33.25171161127876	34.0	34.0	34.0	31.0	34.0
4	36.54461433486459	37.0	37.0	37.0	35.0	37.0
5	36.53627539546774	37.0	37.0	37.0	35.0	37.0
6	36.65026524689104	37.0	37.0	37.0	35.0	37.0
7	36.48045149633844	37.0	37.0	37.0	35.0	37.0
8	36.51181501912468	37.0	37.0	37.0	35.0	37.0
9	38.295108309147174	39.0	39.0	39.0	37.0	39.0
10	38.09846233686355	39.0	39.0	39.0	35.0	39.0
11	37.310855901743324	39.0	35.0	39.0	35.0	39.0
12	37.200713091279525	39.0	35.0	39.0	35.0	39.0
13	37.17895419685932	39.0	35.0	39.0	35.0	39.0
14	38.418497126492014	40.0	35.0	41.0	35.0	41.0
15	38.48822726661157	41.0	35.0	41.0	35.0	41.0
16	38.49901012935591	40.0	35.0	41.0	35.0	41.0
17	38.548053895092934	40.0	36.0	41.0	35.0	41.0
18	38.543881061756395	40.0	36.0	41.0	35.0	41.0
19	38.45701270494167	40.0	35.0	41.0	35.0	41.0
20	38.37729352067196	40.0	35.0	41.0	35.0	41.0
21	38.32815461202837	40.0	35.0	41.0	35.0	41.0
22	38.28934399446441	40.0	35.0	41.0	35.0	41.0
23	38.294258750264284	40.0	35.0	41.0	35.0	41.0
24	38.23269648451765	40.0	35.0	41.0	35.0	41.0
25	38.18913544890153	40.0	35.0	41.0	35.0	41.0
26	38.14001864416553	40.0	35.0	41.0	35.0	41.0
27	38.11027158975148	40.0	35.0	41.0	35.0	41.0
28	38.13243508178446	40.0	35.0	41.0	35.0	41.0
29	38.149123532012226	40.0	35.0	41.0	35.0	41.0
30	38.02068733542199	40.0	35.0	41.0	35.0	41.0
31	37.981012743383246	40.0	35.0	41.0	35.0	41.0
32	37.894567243931036	40.0	35.0	41.0	35.0	41.0
33	37.789483345186156	40.0	35.0	41.0	35.0	41.0
34	37.6858602264209	40.0	35.0	41.0	35.0	41.0
35	37.637741941684126	40.0	35.0	41.0	35.0	41.0
36	37.591640878774484	40.0	35.0	41.0	35.0	41.0
37	37.54116228112326	40.0	35.0	41.0	35.0	41.0
38	37.502629403963326	39.0	35.0	41.0	35.0	41.0
39	37.40466200242182	39.0	35.0	41.0	34.0	41.0
40	37.3280037288331	39.0	35.0	41.0	34.0	41.0
41	37.29380513963903	39.0	35.0	41.0	34.0	41.0
42	37.17993541814827	39.0	35.0	41.0	34.0	41.0
43	37.0764045591712	38.0	35.0	41.0	34.0	41.0
44	36.97363676552559	38.0	35.0	41.0	34.0	41.0
45	36.92386357083822	38.0	35.0	41.0	34.0	41.0
46	36.95073423414765	38.0	35.0	41.0	34.0	41.0
47	36.88230918561516	38.0	35.0	41.0	34.0	41.0
48	36.91635785265343	38.0	35.0	41.0	34.0	41.0
49	36.83599669402426	37.0	35.0	41.0	34.0	41.0
50	36.744706594652776	37.0	35.0	41.0	34.0	41.0
51	36.66170161646837	37.0	35.0	41.0	34.0	41.0
52	36.43508178445807	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	3.0
15	9.0
16	15.0
17	41.0
18	77.0
19	172.0
20	326.0
21	523.0
22	819.0
23	1292.0
24	2155.0
25	3330.0
26	4748.0
27	5715.0
28	5958.0
29	6699.0
30	8034.0
31	10117.0
32	12717.0
33	18087.0
34	54535.0
35	233069.0
36	38064.0
37	62255.0
38	107890.0
39	460574.0
40	3313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.75597286024564	22.657081899782806	24.373402271897284	20.213542968074268
2	27.115151748130778	24.28950352701482	26.126818767178577	22.468525957675823
3	26.871047725219597	25.186057239510255	25.331654717742712	22.611240317527436
4	26.046379764353123	27.0689257500913	22.83372095258231	24.050973532973263
5	24.17225671286063	36.21619543698464	20.118976685182695	19.492571164972034
6	87.0905491379476	2.462183097237973	7.585676667883984	2.86159109693044
7	87.1426374766948	2.455744132854095	5.535971706998289	4.865646683452822
8	76.61598785246122	4.6125088896150075	11.886328252638053	6.885175005285717
9	52.91858073692506	16.734964537643915	14.616545255348184	15.729909470082843
10	42.43902204624522	16.878832144847866	24.163799565610162	16.51834624329675
11	38.46733426874508	15.111000057662368	27.023564687566072	19.39810098602649
12	20.656293847425374	30.621023699233092	30.446691141138256	18.27599131220328
13	14.555327041728333	34.35543083399004	32.35618044476906	18.73306167951256
14	13.018048321064063	20.438618409671903	47.7959521017933	18.747381167470735
15	15.782286120668115	16.140177215676477	47.06604263171046	21.011494031944952
16	16.929478924404638	19.71745439867761	28.071482115055645	35.28158456186211
17	19.510927018663388	21.330270820919907	35.35721836738617	23.80158379303054
18	24.33601783689238	25.546639245007402	28.014588579007054	22.102754339093163
19	25.771138062928863	21.926980221807906	26.28116170449959	26.020720010763643
20	20.496472985180773	30.115420839179656	27.491879216560633	21.89622695907894
21	21.141522670920867	22.772022219232323	26.661541122878507	29.424913986968303
22	18.212370499932728	28.027370403828783	24.77050377688508	28.98975531935341
23	19.35466200242182	21.3213331539393	36.76293078593807	22.561074057700807
24	19.68737386357084	20.28735079862379	34.70150114363696	25.323774194168415
25	15.986507005977666	28.098487323889522	30.307148211505563	25.607857458627254
26	18.22044323139908	27.286024564168603	28.0747496492206	26.418782555211717
27	19.587137447863608	24.570319257308707	35.87416149307091	19.96838180175678
28	17.669190996982337	23.124531493263113	32.214138812539645	26.992138697214905
29	22.701866338631866	25.40354046937167	28.525381052146003	23.36921213985046
30	27.164645280335208	23.100505506756107	25.603917196840104	24.13093201606858
31	30.46485478693755	21.519883906433197	25.649950986987523	22.365310319641722
32	28.72777596248102	22.823437830357314	24.953101274338323	23.49568493282334
33	20.368078113287332	21.15151748130778	32.496588309916	25.983816095488883
34	20.212005304937822	20.923751129221365	32.7205105041613	26.143733061679512
35	29.423760739615968	18.768331827704845	29.446056855094472	22.361850577584715
36	23.64166682683991	23.40592384723317	27.97922232686874	24.973186999058182
37	20.513483383627733	25.426221000634285	27.365022007803642	26.695273607934343
38	19.85161550733273	23.967747515712993	24.98923635804486	31.191400618909416
39	25.50771714686605	19.78021027543391	25.3305975743364	29.38147500336364
40	16.83866069540815	18.614757721952063	30.363272915985927	34.18330866665386
41	22.193476464143618	22.507159743979088	24.675553078209393	30.623810713667904
42	21.803390547215866	18.944874776558326	29.50054779249236	29.751186883733443
43	24.082495627270458	22.40548176908144	29.630768639360333	23.881253964287772
44	18.932765679358795	27.446133738251294	27.564533799757818	26.056566782632096
45	22.716858554212234	23.91796567167048	28.807157821900166	24.558017952217117
46	23.726911026966768	19.690256981951677	28.342879658638783	28.23995233244277
47	21.93851269533127	17.388279162742425	32.32927133988122	28.343936802045093
48	21.47606050704442	16.6121436946201	29.936283083783422	31.975512714552057
49	21.158725277259887	21.633574874584347	31.233590251215716	25.97410959694005
50	19.848636285005863	23.317316008995327	28.391412151382934	28.442635554615876
51	19.794049243661945	18.7661214369462	31.341995502335322	30.09783381705653
52	22.00222961154785	17.242873892402024	34.65825436792434	26.09664212812578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	110.0
1	299.5
2	489.0
3	2093.0
4	3697.0
5	2463.5
6	1230.0
7	1346.5
8	1463.0
9	1533.0
10	1603.0
11	1557.5
12	1512.0
13	1464.0
14	1317.5
15	1219.0
16	1225.5
17	1232.0
18	1322.0
19	1412.0
20	1427.5
21	1443.0
22	1421.5
23	1400.0
24	1926.0
25	2452.0
26	2807.0
27	3162.0
28	3714.0
29	4266.0
30	4933.5
31	5601.0
32	6683.5
33	7766.0
34	9018.5
35	10271.0
36	12173.0
37	14075.0
38	16225.0
39	21560.5
40	24746.0
41	34971.0
42	45196.0
43	60889.5
44	76583.0
45	82879.0
46	89175.0
47	90065.0
48	90955.0
49	104598.0
50	118241.0
51	120605.0
52	122969.0
53	111124.0
54	99279.0
55	83837.0
56	68395.0
57	59735.0
58	51075.0
59	47295.0
60	43515.0
61	42276.0
62	41037.0
63	36192.5
64	25922.5
65	20497.0
66	16652.5
67	12808.0
68	9924.0
69	7040.0
70	6086.5
71	5133.0
72	4263.5
73	3394.0
74	2951.0
75	2508.0
76	1870.0
77	1232.0
78	984.5
79	737.0
80	452.5
81	168.0
82	225.0
83	282.0
84	153.5
85	25.0
86	14.5
87	4.0
88	3.0
89	1.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1040540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.906992840797646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.44563724379918	15.449767294867428
2	13.064618694401368	5.985422538008065
3	5.553635077477146	3.81651236880515
4	2.7850325440192827	2.5518688218895265
5	1.58524068571539	1.8156548519311793
6	0.9967692429663497	1.369979154753448
7	0.7139617717990148	1.1448302036642264
8	0.4854601162176906	0.8896345125353144
9	0.3954340284934536	0.8152384013736582
>10	5.098206778576306	31.112508595916843
>50	1.649756327509104	24.236497251050185
>100	0.2165751986027366	8.024051710557467
>500	0.005887481127064684	0.9535882621351368
>1k	0.0037848092959701543	1.8344460325123153
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	4656	0.4474599727064793	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT	3344	0.32137159551771194	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	3307	0.31781574951467506	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1851	0.17788840409787227	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1380	0.132623445518673	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	1251	0.1202260364810579	TruSeq Adapter, Index 13 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1190	0.11436369577334846	TruSeq Adapter, Index 13 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1059	0.10177407884367731	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33059757433640224	0.0
2	0.0	0.0	0.0	1.338631864224345	0.0
3	0.0	0.0	0.0	2.134084225498299	0.0
4	0.0	0.0	0.0	2.6314221461933225	0.0
5	0.0	0.0	0.0	5.504737924539182	0.0
6	0.0	0.0	0.0	6.016299229246353	0.0
7	0.0	0.0	0.0	8.492321294712362	0.0
8	0.0	0.0	0.0	11.23089934072693	0.0
9	0.0	0.0	0.0	13.82205393353451	0.0
10	0.0	0.0	0.0	15.047187037499759	0.0
11	0.0	0.0	0.0	16.31441366982528	0.0
12	0.0	0.0	0.0	17.341092125242664	0.0
13	0.0	0.0	0.0	17.738481942068542	0.0
14	9.610394602802391E-5	0.0	0.0	18.03477040767294	0.0
15	9.610394602802391E-5	0.0	0.0	19.047129375132144	0.0
16	9.610394602802391E-5	0.0	0.0	20.154823457051148	0.0
17	9.610394602802391E-5	0.0	0.0	21.807426912949047	0.0
18	9.610394602802391E-5	0.0	0.0	22.488227266611567	0.0
19	1.9220789205604783E-4	0.0	0.0	23.4379264612605	0.0
20	1.9220789205604783E-4	0.0	0.0	24.38368539412228	0.0
21	1.9220789205604783E-4	0.0	0.0	25.16616372268245	0.0
22	1.9220789205604783E-4	0.0	0.0	26.072520037672746	0.0
23	2.8831183808407173E-4	0.0	0.0	26.696234647394622	0.0
24	2.8831183808407173E-4	0.0	0.0	27.222692063736137	0.0
25	2.8831183808407173E-4	0.0	0.0	27.78038326253676	0.0
26	2.8831183808407173E-4	0.0	0.0	28.263497799219635	0.0
27	2.8831183808407173E-4	0.0	0.0	28.784765602475638	0.0
28	2.8831183808407173E-4	0.0	0.0	29.232321679128145	0.0
29	2.8831183808407173E-4	0.0	0.0	29.87544928594768	0.0
30	2.8831183808407173E-4	0.0	0.0	30.490130124742922	0.0
31	2.8831183808407173E-4	0.0	0.0	30.997366751878833	0.0
32	2.8831183808407173E-4	0.0	0.0	31.53055144444231	0.0
33	3.8441578411209566E-4	0.0	0.0	32.02029715340112	0.0
34	3.8441578411209566E-4	0.0	0.0	32.71868452918677	0.0
35	3.8441578411209566E-4	0.0	0.0	33.22563284448459	0.0
36	3.8441578411209566E-4	0.0	0.0	33.71537855344341	0.0
37	3.8441578411209566E-4	0.0	0.0	34.20531647029427	0.0
38	3.8441578411209566E-4	0.0	0.0	34.68919983854537	0.0
39	3.8441578411209566E-4	0.0	0.0	35.268130009418186	0.0
40	3.8441578411209566E-4	0.0	0.0	35.84869394737348	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	6.311176E-4	46.000004	45
GTACCGT	20	6.311176E-4	46.000004	17
GTACCGG	20	6.311176E-4	46.000004	1
CTCGTTA	20	6.311176E-4	46.000004	44
TTTACGG	20	6.311176E-4	46.000004	1
CGTTAGG	20	6.311176E-4	46.000004	1
ACTAGTG	20	6.311176E-4	46.000004	32
CTAACGG	145	0.0	46.000004	1
ACGGCTA	20	6.311176E-4	46.000004	14
ACGTGCG	20	6.311176E-4	46.000004	10
CGACTAG	20	6.311176E-4	46.000004	29
GCGTATT	20	6.311176E-4	46.000004	39
TCACGTA	20	6.311176E-4	46.000004	32
ACTATAG	30	1.8612318E-6	46.0	32
TAAACGG	55	1.8189894E-12	46.0	1
GTTAGAC	25	3.416931E-5	46.0	10
CACGATA	30	1.8612318E-6	46.0	18
ACGGAAT	45	3.092282E-10	46.0	4
CGAATAT	55	1.8189894E-12	46.0	14
ACGTAGG	110	0.0	46.0	1
>>END_MODULE