FastQCFastQC Report
Thu 26 May 2016
SRR1544987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544987_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences547861
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT29950.5466715097442599TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT21670.3955382843458468Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT20750.3787457037460232TruSeq Adapter, Index 13 (95% over 22bp)
TGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTTG13980.25517421389732065TruSeq Adapter, Index 13 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA11630.21228012214777103No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.19804293424792052No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC7790.14218935094850702No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT7100.12959491549863925TruSeq Adapter, Index 13 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC6670.12174620934872166TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC6410.1170004800487715No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG5570.10166812384893248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGG1400.046.0000041
CTAGCGG1400.046.0000041
TAATATG253.4147975E-546.014
CGGGTAT206.3085486E-446.05
ATCTCGG206.3085486E-446.01
ATCTCGC206.3085486E-446.040
ACGTTCA253.4147975E-546.025
TAGCGGC206.3085486E-446.02
GGGTACG206.3085486E-446.07
ATAGCGG800.046.01
GAATCGG206.3085486E-446.045
TAAACGG253.4147975E-546.01
GGATCGT253.4147975E-546.07
CACGACG501.6370905E-1146.025
ATCACGC206.3085486E-446.010
TACGCCC253.4147975E-546.034
ACGGAGT206.3085486E-446.029
TTACGGG850.046.02
CGGTCTA501.6370905E-1146.030
ACCGTAC206.3085486E-446.030
Produced by FastQC (version 0.11.5)