##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1544987_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 547861 Sequences flagged as poor quality 0 Sequence length 52 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.926209385227274 34.0 33.0 34.0 31.0 34.0 2 33.266113119933706 34.0 33.0 34.0 31.0 34.0 3 33.386316967259944 34.0 34.0 34.0 31.0 34.0 4 36.61233962629207 37.0 37.0 37.0 35.0 37.0 5 36.60854669341311 37.0 37.0 37.0 35.0 37.0 6 36.420765486136084 37.0 37.0 37.0 35.0 37.0 7 36.258342900845285 37.0 37.0 37.0 35.0 37.0 8 36.3905370157759 37.0 37.0 37.0 35.0 37.0 9 38.2757652032176 39.0 39.0 39.0 37.0 39.0 10 38.14746076103245 39.0 39.0 39.0 35.0 39.0 11 37.41625156745963 39.0 35.0 39.0 35.0 39.0 12 37.36656743225015 39.0 35.0 39.0 35.0 39.0 13 37.35593517333776 39.0 35.0 39.0 35.0 39.0 14 38.52849536652545 41.0 35.0 41.0 35.0 41.0 15 38.57050054667151 41.0 35.0 41.0 35.0 41.0 16 38.60393786015066 41.0 35.0 41.0 35.0 41.0 17 38.680566786100854 41.0 36.0 41.0 35.0 41.0 18 38.68882983092427 41.0 36.0 41.0 35.0 41.0 19 38.62948266074789 41.0 35.0 41.0 35.0 41.0 20 38.55927689687713 41.0 35.0 41.0 35.0 41.0 21 38.51749257567157 40.0 35.0 41.0 35.0 41.0 22 38.47233878666304 40.0 35.0 41.0 35.0 41.0 23 38.44859188735829 40.0 35.0 41.0 35.0 41.0 24 38.41383672135815 40.0 35.0 41.0 35.0 41.0 25 38.355623050372266 40.0 35.0 41.0 35.0 41.0 26 38.30387817347831 40.0 35.0 41.0 35.0 41.0 27 38.358897603589234 40.0 35.0 41.0 35.0 41.0 28 38.36524227860716 40.0 35.0 41.0 35.0 41.0 29 38.39003506363841 40.0 36.0 41.0 35.0 41.0 30 38.24805927050839 40.0 35.0 41.0 35.0 41.0 31 38.22425761278865 40.0 35.0 41.0 35.0 41.0 32 38.1328274872641 40.0 35.0 41.0 35.0 41.0 33 38.03448684976664 40.0 35.0 41.0 35.0 41.0 34 37.91944124513335 40.0 35.0 41.0 35.0 41.0 35 37.89323204243412 40.0 35.0 41.0 35.0 41.0 36 37.865987905691405 40.0 35.0 41.0 35.0 41.0 37 37.81512828983994 40.0 35.0 41.0 35.0 41.0 38 37.794444211214156 40.0 35.0 41.0 35.0 41.0 39 37.739578104665235 40.0 35.0 41.0 35.0 41.0 40 37.687292944743284 40.0 35.0 41.0 35.0 41.0 41 37.64581709594222 40.0 35.0 41.0 35.0 41.0 42 37.55529961066767 39.0 35.0 41.0 35.0 41.0 43 37.49173421725584 39.0 35.0 41.0 35.0 41.0 44 37.33582423278897 39.0 35.0 41.0 35.0 41.0 45 37.334886038611984 39.0 35.0 41.0 35.0 41.0 46 37.28138341659655 39.0 35.0 41.0 35.0 41.0 47 37.188931133992014 39.0 35.0 41.0 35.0 41.0 48 37.167077050565744 38.0 35.0 41.0 35.0 41.0 49 37.09428121366551 38.0 35.0 41.0 35.0 41.0 50 37.00874126831441 38.0 35.0 41.0 35.0 41.0 51 36.93105185439372 37.0 35.0 41.0 34.0 41.0 52 36.45538010553772 37.0 35.0 40.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 1.0 13 1.0 14 9.0 15 4.0 16 11.0 17 15.0 18 38.0 19 74.0 20 123.0 21 216.0 22 341.0 23 547.0 24 882.0 25 1566.0 26 2190.0 27 2580.0 28 2532.0 29 2702.0 30 3151.0 31 3765.0 32 5089.0 33 7614.0 34 26856.0 35 120483.0 36 18968.0 37 31960.0 38 57955.0 39 257007.0 40 1179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.65080011170717 22.007406988268922 23.34497253865488 21.996820361369032 2 26.7237492721694 24.67833994389088 26.192957702774972 22.40495308116475 3 27.024920554666238 25.005065153387445 25.475987522382503 22.494026769563813 4 25.938331073027648 26.719733655069444 23.227972058606106 24.113963213296806 5 24.04588025064752 35.60373890457616 20.500455407484743 19.849925437291578 6 85.6281063992509 2.549734330423228 8.370371316812111 3.451787953513756 7 85.73086969139982 2.4020691379747783 6.360554958283214 5.506506212342182 8 74.82372353571435 4.746276884100164 12.94105621681412 7.488943363371366 9 52.47790954274898 16.243353697379444 15.166255674340754 16.11248108553082 10 41.54119384296382 16.650208720825173 25.17810174478563 16.630495691425377 11 37.46296962185664 15.289279580039464 27.195036697264452 20.05271410083945 12 20.15146177588841 30.044482085784534 31.01352350322436 18.7905326351027 13 14.607354785246624 33.65908505989658 32.667045108157 19.066515046699802 14 12.98668822931364 20.38947835308591 47.07453167865572 19.54930173894473 15 15.684817864385309 16.233862238779544 46.8379753258582 21.243344570976944 16 16.852814856323047 20.236702375237513 28.121914135154718 34.788568633284726 17 19.319498923997145 21.68378475562232 35.1145637305813 23.882152589799237 18 23.929062298648745 25.8667800774284 27.893571544607116 22.31058607931574 19 25.579663454781414 21.98605120641915 25.960964551227413 26.473320787572028 20 20.201291933537888 30.465756825180108 27.271698478263644 22.06125276301836 21 21.145327008127975 22.743542613911195 26.763540387068986 29.347589990891848 22 18.44299922790635 28.234716470053534 24.69714033304068 28.625143968999435 23 19.500749277645244 21.445220594274826 36.46746163716709 22.586568490912843 24 20.129010825738643 20.509216753884655 33.86333394784444 25.498438472532264 25 16.343561596828394 27.989581298906106 30.325210226681587 25.341646877583912 26 18.789072410702715 26.29991914007385 28.06952858480527 26.841479864418165 27 20.435840477785426 24.06267283124734 35.555368971326665 19.946117719640565 28 17.988139327311124 22.69882324166166 32.57067029775801 26.742367133269205 29 23.36359039975468 24.56279968824209 28.38019132590201 23.69341858610122 30 27.1234857016652 22.69626784896169 25.498255944482267 24.681990504890837 31 30.57636882347895 21.367463644975643 25.18686309118554 22.869304440359873 32 28.51854758780056 22.3239106269656 25.3047762114843 23.852765573749547 33 20.078633083939174 20.774612538581867 32.972962119953785 26.173792257525175 34 20.943268456780096 20.45555350718522 32.66594993985701 25.93522809617768 35 29.007175177645422 18.48406803915592 29.778538716937327 22.730218066261333 36 23.8000149673001 22.92753088830926 27.652999574709643 25.619454569680993 37 20.77443001053187 24.98297925933768 27.52468235556099 26.71790837456946 38 19.79498449424215 23.67808622990138 24.652603488841148 31.874325787015316 39 25.76931009872942 19.68382491179332 25.109653726036345 29.43721126344091 40 16.987885613321627 18.248971910758385 30.450972053130265 34.31217042278972 41 22.456608519314205 22.602448431262676 24.24574846539542 30.695194584027703 42 21.922531445019814 18.95517293620097 29.356168809241762 29.766126809537457 43 24.466059821743105 22.526516762463473 29.170172726293714 23.837250689499708 44 19.10101284814944 26.582655089520884 28.03046758210568 26.285864480223996 45 22.69991840996165 23.520564522753034 28.646317222799212 25.133199844486104 46 23.85440832619953 19.244479895447935 28.477296248500988 28.42381552985155 47 21.99116199181909 17.010884147621386 32.620682983457485 28.37727087710204 48 21.31343534217621 16.44924533777728 29.88038936883626 32.35692995121025 49 20.9237379554303 21.114662295728294 31.69325796141722 26.26834178742418 50 19.77052573554241 23.2476850880059 28.65343581674914 28.32835335970255 51 19.911984974290924 18.570586334855008 31.157173078572846 30.360255612281218 52 22.13243870251761 17.318808967968153 34.60293760643667 25.94581472307757 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55.0 1 188.0 2 321.0 3 1246.0 4 2171.0 5 1478.0 6 785.0 7 842.5 8 900.0 9 898.0 10 896.0 11 905.5 12 915.0 13 896.5 14 789.5 15 701.0 16 692.0 17 683.0 18 740.5 19 798.0 20 796.0 21 794.0 22 785.5 23 777.0 24 1034.5 25 1292.0 26 1408.5 27 1525.0 28 1815.5 29 2106.0 30 2410.0 31 2714.0 32 3288.0 33 3862.0 34 4422.0 35 4982.0 36 5944.5 37 6907.0 38 8178.0 39 10802.0 40 12155.0 41 17194.5 42 22234.0 43 30169.5 44 38105.0 45 41717.5 46 45330.0 47 46089.0 48 46848.0 49 55546.0 50 64244.0 51 65374.0 52 66504.0 53 60011.0 54 53518.0 55 44870.5 56 36223.0 57 31492.0 58 26761.0 59 25007.5 60 23254.0 61 22558.0 62 21862.0 63 19767.0 64 14382.0 65 11092.0 66 9087.0 67 7082.0 68 5498.0 69 3914.0 70 3323.0 71 2732.0 72 2340.5 73 1949.0 74 1672.5 75 1396.0 76 1077.0 77 758.0 78 577.5 79 397.0 80 250.5 81 104.0 82 144.0 83 184.0 84 106.0 85 28.0 86 14.5 87 1.0 88 2.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 547861.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.299966938317922 #Duplication Level Percentage of deduplicated Percentage of total 1 70.73918135387949 20.01916493558465 2 11.561980493582013 6.544073314196955 3 4.186334120526675 3.5541935161097147 4 2.008266553855047 2.2733550830971 5 1.1666472612294398 1.650803946073615 6 0.7529130802337247 1.278444916682491 7 0.6062125097643475 1.2009055788748033 8 0.5173163090811176 1.1712027554918625 9 0.43647675952369397 1.1117050077478214 >10 7.622869512768318 48.44764754593225 >50 0.31057162941534666 5.897347844972512 >100 0.08281910117742579 4.048693085359929 >500 0.004529169595640472 0.8097019955434672 >1k 0.003882145367691834 1.9927604743327803 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT 2995 0.5466715097442599 TruSeq Adapter, Index 13 (95% over 22bp) CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT 2167 0.3955382843458468 Illumina Single End Adapter 1 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT 2075 0.3787457037460232 TruSeq Adapter, Index 13 (95% over 22bp) TGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTTG 1398 0.25517421389732065 TruSeq Adapter, Index 13 (95% over 24bp) CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA 1163 0.21228012214777103 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1085 0.19804293424792052 No Hit ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC 779 0.14218935094850702 No Hit TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT 710 0.12959491549863925 TruSeq Adapter, Index 13 (95% over 22bp) GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC 667 0.12174620934872166 TruSeq Adapter, Index 13 (95% over 21bp) CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC 641 0.1170004800487715 No Hit ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG 557 0.10166812384893248 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.404664686845751 0.0 2 0.0 0.0 0.0 1.5870813947333355 0.0 3 0.0 0.0 0.0 2.493515691023818 0.0 4 0.0 0.0 0.0 3.0478533788679973 0.0 5 0.0 0.0 0.0 6.131482255535619 0.0 6 0.0 0.0 0.0 6.679431461629866 0.0 7 0.0 0.0 0.0 9.389790476051408 0.0 8 0.0 0.0 0.0 12.229196821821594 0.0 9 0.0 0.0 0.0 14.857418213743998 0.0 10 0.0 0.0 0.0 16.09696620128098 0.0 11 0.0 0.0 0.0 17.344545423017884 0.0 12 0.0 0.0 0.0 18.426024119256528 0.0 13 0.0 0.0 0.0 18.83616464760222 0.0 14 0.0 0.0 0.0 19.135693177649074 0.0 15 0.0 0.0 0.0 20.162048402788297 0.0 16 0.0 0.0 0.0 21.22198878912717 0.0 17 0.0 0.0 0.0 22.723099472311407 0.0 18 0.0 0.0 0.0 23.389874438954408 0.0 19 0.0 0.0 0.0 24.299776768194853 0.0 20 0.0 0.0 0.0 25.236693248835014 0.0 21 0.0 0.0 0.0 25.987978702627125 0.0 22 0.0 0.0 0.0 26.856629692568006 0.0 23 0.0 0.0 0.0 27.449845855061778 0.0 24 0.0 0.0 0.0 27.96366231580638 0.0 25 0.0 0.0 0.0 28.49646169375079 0.0 26 0.0 0.0 0.0 28.969026815195825 0.0 27 0.0 0.0 0.0 29.468241031940583 0.0 28 0.0 0.0 0.0 29.893531388436116 0.0 29 0.0 0.0 0.0 30.471232666680052 0.0 30 0.0 0.0 0.0 31.056600123023905 0.0 31 0.0 0.0 0.0 31.529347772518943 0.0 32 0.0 0.0 0.0 32.03695827956361 0.0 33 0.0 0.0 0.0 32.51244384980862 0.0 34 0.0 0.0 0.0 33.16917977370172 0.0 35 1.8252804999808344E-4 0.0 0.0 33.642292479296756 0.0 36 1.8252804999808344E-4 0.0 0.0 34.10299327749192 0.0 37 1.8252804999808344E-4 0.0 0.0 34.56825727693703 0.0 38 1.8252804999808344E-4 0.0 0.0 35.01709375188232 0.0 39 1.8252804999808344E-4 0.0 0.0 35.54587751272677 0.0 40 3.650560999961669E-4 0.0 0.0 36.10258806522092 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTAGG 140 0.0 46.000004 1 CTAGCGG 140 0.0 46.000004 1 TAATATG 25 3.4147975E-5 46.0 14 CGGGTAT 20 6.3085486E-4 46.0 5 ATCTCGG 20 6.3085486E-4 46.0 1 ATCTCGC 20 6.3085486E-4 46.0 40 ACGTTCA 25 3.4147975E-5 46.0 25 TAGCGGC 20 6.3085486E-4 46.0 2 GGGTACG 20 6.3085486E-4 46.0 7 ATAGCGG 80 0.0 46.0 1 GAATCGG 20 6.3085486E-4 46.0 45 TAAACGG 25 3.4147975E-5 46.0 1 GGATCGT 25 3.4147975E-5 46.0 7 CACGACG 50 1.6370905E-11 46.0 25 ATCACGC 20 6.3085486E-4 46.0 10 TACGCCC 25 3.4147975E-5 46.0 34 ACGGAGT 20 6.3085486E-4 46.0 29 TTACGGG 85 0.0 46.0 2 CGGTCTA 50 1.6370905E-11 46.0 30 ACCGTAC 20 6.3085486E-4 46.0 30 >>END_MODULE