FastQCFastQC Report
Thu 26 May 2016
SRR1544986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544986_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences874578
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT36290.4149429782134927Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT31110.3557144131226717Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTT27280.3119218640304238Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15210.1739124469172561No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC13060.14932916217878794No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT12850.14692800413456547Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA12340.14109662031288234No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA12110.13846678055016248No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11670.13343578274322015No Hit
GCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC9530.10896683886400069No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC9490.10850947542700594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC750.046.0000045
AAGTACG351.01919795E-746.00000419
CGTTTTA351.01919795E-746.00000446
AGTACGT351.01919795E-746.00000420
GTACGTC351.01919795E-746.00000421
TACGTCA351.01919795E-746.00000422
ACAACGA453.092282E-1046.012
CTGTCGA206.3106226E-446.011
ATCTCGC253.416482E-546.013
CCGGGTA501.6370905E-1146.04
GTACCGA206.3106226E-446.07
CTGTACG253.416482E-546.034
TTAGCTA206.3106226E-446.033
CGACGGT206.3106226E-446.027
GGGTACG253.416482E-546.06
TAGCGAG253.416482E-546.026
CGACATA206.3106226E-446.041
CGATTGA253.416482E-546.09
GCGTTTC301.8608898E-646.012
CGACAAA206.3106226E-446.018
Produced by FastQC (version 0.11.5)