##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544986_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	874578
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90836037494655	34.0	33.0	34.0	31.0	34.0
2	33.20284983157591	34.0	33.0	34.0	31.0	34.0
3	33.251163418242854	34.0	33.0	34.0	31.0	34.0
4	36.55295010850948	37.0	37.0	37.0	35.0	37.0
5	36.54160978208919	37.0	37.0	37.0	35.0	37.0
6	36.64976480085252	37.0	37.0	37.0	35.0	37.0
7	36.47503710360883	37.0	37.0	37.0	35.0	37.0
8	36.516935024663326	37.0	37.0	37.0	35.0	37.0
9	38.312013336717825	39.0	39.0	39.0	37.0	39.0
10	38.11215580542844	39.0	39.0	39.0	35.0	39.0
11	37.30167120599878	39.0	35.0	39.0	35.0	39.0
12	37.185608373409806	39.0	35.0	39.0	35.0	39.0
13	37.16729096775816	39.0	35.0	39.0	35.0	39.0
14	38.42249633537546	40.0	35.0	41.0	35.0	41.0
15	38.50403623233148	41.0	35.0	41.0	35.0	41.0
16	38.516558843236396	41.0	35.0	41.0	35.0	41.0
17	38.609293853721454	41.0	36.0	41.0	35.0	41.0
18	38.593686326434	40.0	36.0	41.0	35.0	41.0
19	38.490176976781946	40.0	35.0	41.0	35.0	41.0
20	38.407413632631965	40.0	35.0	41.0	35.0	41.0
21	38.353958137524614	40.0	35.0	41.0	35.0	41.0
22	38.3239333712945	40.0	35.0	41.0	35.0	41.0
23	38.32666611783054	40.0	35.0	41.0	35.0	41.0
24	38.27849431382907	40.0	35.0	41.0	35.0	41.0
25	38.237501972379825	40.0	35.0	41.0	35.0	41.0
26	38.1857147104089	40.0	35.0	41.0	35.0	41.0
27	38.194151922412864	40.0	35.0	41.0	35.0	41.0
28	38.2285422226491	40.0	35.0	41.0	35.0	41.0
29	38.235460988042234	40.0	35.0	41.0	35.0	41.0
30	38.14728589102401	40.0	35.0	41.0	35.0	41.0
31	38.07996999695853	40.0	35.0	41.0	35.0	41.0
32	38.0183539947266	40.0	35.0	41.0	35.0	41.0
33	37.915870282582	40.0	35.0	41.0	35.0	41.0
34	37.8472177438719	40.0	35.0	41.0	35.0	41.0
35	37.802956397256736	40.0	35.0	41.0	35.0	41.0
36	37.75120686776937	40.0	35.0	41.0	35.0	41.0
37	37.71774730212743	40.0	35.0	41.0	35.0	41.0
38	37.6659760478768	40.0	35.0	41.0	35.0	41.0
39	37.573792160333326	40.0	35.0	41.0	35.0	41.0
40	37.50932335366314	40.0	35.0	41.0	34.0	41.0
41	37.47995947759948	40.0	35.0	41.0	34.0	41.0
42	37.38539844359222	39.0	35.0	41.0	34.0	41.0
43	37.307869623978654	39.0	35.0	41.0	34.0	41.0
44	37.26075775974241	39.0	35.0	41.0	34.0	41.0
45	37.22096371049809	39.0	35.0	41.0	34.0	41.0
46	37.240435958828144	39.0	35.0	41.0	34.0	41.0
47	37.210924583056055	39.0	35.0	41.0	35.0	41.0
48	37.23774780522721	39.0	35.0	41.0	35.0	41.0
49	37.176192403650674	39.0	35.0	41.0	34.0	41.0
50	37.105365101797666	39.0	35.0	41.0	34.0	41.0
51	37.04085970605252	38.0	35.0	41.0	34.0	41.0
52	36.852318489602986	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	4.0
14	11.0
15	11.0
16	18.0
17	39.0
18	83.0
19	127.0
20	248.0
21	413.0
22	628.0
23	974.0
24	1797.0
25	2939.0
26	3964.0
27	4627.0
28	4612.0
29	5001.0
30	5976.0
31	7336.0
32	9649.0
33	14312.0
34	41947.0
35	203019.0
36	27731.0
37	41020.0
38	74830.0
39	419334.0
40	3923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.45816839664386	22.699290400627504	24.85827450496125	19.98426669776738
2	26.834427575356344	24.62719162841965	26.308459622812375	22.229921173411636
3	26.342647539727732	25.170082028132423	25.64436791229599	22.842902519843854
4	25.958233570933636	26.98146992035016	23.537408898920393	23.52288760979581
5	22.84335988328085	35.594881188413154	21.03014253731514	20.531616390990855
6	85.94888048864709	3.2481951295367595	7.802277212552797	3.0006471692633476
7	86.13365531719298	2.708048910445952	5.725046822581863	5.433248949779208
8	75.92347394972204	4.707070152690783	12.084228050556954	7.2852278470302245
9	50.27098783641939	18.901573101541544	15.664812057929653	15.16262700410941
10	43.90437445259314	18.679980516317585	25.807989681880862	11.607655349208418
11	42.2672420298704	16.80250360745411	23.60338357470689	17.3268707879686
12	20.339638088312306	35.85443493890768	27.634927930956415	16.1709990418236
13	13.491992709626816	38.678882844068795	28.821671709098563	19.007452737205828
14	12.123332624420007	22.93860581903501	46.96104864288834	17.977012913656644
15	13.124729869720026	17.598315987824986	48.62733798471949	20.6496161577355
16	13.314993059509844	20.948045800374583	29.175442327614004	36.56151881250157
17	17.75393389726245	22.471409068144865	35.56606729188248	24.2085897427102
18	23.41803704186476	27.729030458118086	27.94925095303106	20.90368154698609
19	26.130430904962164	22.950497268396873	25.929076651825223	24.98999517481574
20	18.088495251424117	32.65609242400335	27.20614970877383	22.049262615798707
21	18.63584494464759	25.70862747519375	25.073235320348786	30.582292259809872
22	16.231714038084654	31.63125530255735	23.113204311107758	29.023826348250243
23	16.394764103373284	21.947956614504367	39.75460164788046	21.902677634241886
24	17.521478930409867	22.667160619178624	35.75815993542028	24.05320051499123
25	13.994177763447057	31.934029897847875	30.237097205738085	23.834695132966985
26	16.13349523999003	31.250271559540714	27.52367427490744	25.092558925561814
27	17.758964895069393	28.30027739092454	36.45483879082255	17.485918923183526
28	15.988511030462691	24.26667489920853	32.51613921228295	27.228674858045824
29	18.146351726203953	31.030165405486986	27.050760481054866	23.7727223872542
30	25.791638939008298	24.529087171184276	25.1589909647853	24.520282925022123
31	29.502571525924502	25.65305781759889	23.295806663327916	21.548563993148694
32	23.42809903747865	27.529162636151376	24.64560050675869	24.397137819611288
33	19.12305134590625	24.91304377654137	28.27900999110428	27.68489488644809
34	18.569870268861095	22.1589154998182	33.480032655749405	25.791181575571304
35	30.427017372950154	21.039632828632783	23.974076640391136	24.559273158025928
36	19.58293028180448	26.12116929536302	30.256192129232613	24.039708293599883
37	20.744404730052665	26.459732579598388	28.986665568994418	23.80919712135453
38	20.510577672889095	26.494034837372997	28.700013034857953	24.295374454879955
39	26.55577890136729	20.842966550725038	28.555943552204603	24.04531099570307
40	15.356777783113685	19.47247701177025	34.22004669680692	30.95069850830915
41	21.26214014073073	23.927082547239927	26.48500190949235	28.325775402536994
42	21.582637569204806	20.828102239022705	29.5638582264818	28.025401965290687
43	22.206138274687905	26.266610868327355	28.171186560832766	23.356064296151974
44	15.339740995085629	30.527522988229748	27.159612979059617	26.973123037625
45	21.680742026440182	27.67883482090791	26.817733809906034	23.822689342745875
46	22.649666467713573	22.089853620831988	29.5159494064566	25.744530504997837
47	21.706240038052638	18.643277100498754	33.8158517593628	25.834631102085805
48	20.742918298882433	17.62392834029669	31.123124524056173	30.510028836764704
49	20.644470819069312	23.767577048588002	31.377189913306758	24.21076221903592
50	18.87127277384064	24.9149875711486	28.875297572086193	27.338442082924562
51	19.273752598396026	20.218665459227193	32.579484048306725	27.928097894070053
52	22.01633244833508	19.049873195987093	34.64722414696002	24.2865702087178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	125.0
1	232.5
2	340.0
3	1650.0
4	2960.0
5	2030.0
6	1100.0
7	1221.0
8	1342.0
9	1453.0
10	1564.0
11	1471.5
12	1379.0
13	1338.0
14	1299.5
15	1302.0
16	1247.5
17	1193.0
18	1120.5
19	1048.0
20	1116.0
21	1184.0
22	1236.0
23	1288.0
24	1803.0
25	2318.0
26	2724.0
27	3130.0
28	4489.5
29	5849.0
30	6164.5
31	6480.0
32	7560.0
33	8640.0
34	10848.0
35	13056.0
36	14907.0
37	16758.0
38	17175.0
39	22376.5
40	27161.0
41	37995.0
42	48829.0
43	65404.0
44	81979.0
45	93094.0
46	104209.0
47	108769.5
48	113330.0
49	111502.5
50	109675.0
51	97427.5
52	85180.0
53	71864.5
54	58549.0
55	47772.5
56	36996.0
57	33378.5
58	29761.0
59	27197.0
60	24633.0
61	23323.0
62	22013.0
63	19970.0
64	13805.0
65	9683.0
66	7532.5
67	5382.0
68	4516.0
69	3650.0
70	2809.0
71	1968.0
72	1770.0
73	1572.0
74	1376.5
75	1181.0
76	878.5
77	576.0
78	390.0
79	204.0
80	156.5
81	109.0
82	76.0
83	43.0
84	26.0
85	9.0
86	5.0
87	1.0
88	3.0
89	5.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	874578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.48674861081887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.68187710575198	15.219453559853818
2	12.92319513529318	5.812012805117902
3	5.453426455633153	3.67889489326235
4	2.7695585143749772	2.4911346430281243
5	1.5989973592924862	1.797812582388668
6	1.0446403048358681	1.4094338354144016
7	0.7308549821660925	1.1504186578453404
8	0.49662540738401384	0.8933992551671863
9	0.34357745286111335	0.6953345829749994
>10	4.9549415096353275	29.605947004821832
>50	1.7404265953668772	25.419156780295705
>100	0.25168932614404066	9.001288556542333
>500	0.005604418193490784	0.8560544260883617
>1k	0.00458543306740155	1.9696584171990248
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	3629	0.4149429782134927	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	3111	0.3557144131226717	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTT	2728	0.3119218640304238	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1521	0.1739124469172561	No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1306	0.14932916217878794	No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	1285	0.14692800413456547	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1234	0.14109662031288234	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1211	0.13846678055016248	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1167	0.13343578274322015	No Hit
GCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	953	0.10896683886400069	No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	949	0.10850947542700594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31638115754112267	0.0
2	0.0	0.0	0.0	1.3508229111640129	0.0
3	0.0	0.0	0.0	2.1915712492196238	0.0
4	0.0	0.0	0.0	2.7252000393332554	0.0
5	0.0	0.0	0.0	5.692002314258991	0.0
6	0.0	0.0	0.0	6.2433539375561695	0.0
7	0.0	0.0	0.0	9.050079009533741	0.0
8	0.0	0.0	0.0	11.918090782068608	0.0
9	0.0	0.0	0.0	14.695658934937764	0.0
10	0.0	0.0	0.0	15.857247724045196	0.0
11	1.1434085924868908E-4	0.0	0.0	17.02501091955206	0.0
12	1.1434085924868908E-4	0.0	0.0	18.016231828378945	0.0
13	2.2868171849737816E-4	0.0	0.0	18.381779555397003	0.0
14	2.2868171849737816E-4	0.0	0.0	18.663515432585772	0.0
15	2.2868171849737816E-4	0.0	0.0	19.684464964817316	0.0
16	2.2868171849737816E-4	0.0	0.0	20.79894531991429	0.0
17	2.2868171849737816E-4	0.0	0.0	22.432418835141064	0.0
18	2.2868171849737816E-4	0.0	0.0	23.041169569781083	0.0
19	2.2868171849737816E-4	0.0	0.0	23.853790056461516	0.0
20	2.2868171849737816E-4	0.0	0.0	24.731127469476707	0.0
21	2.2868171849737816E-4	0.0	0.0	25.476058167481916	0.0
22	2.2868171849737816E-4	0.0	0.0	26.36025603205203	0.0
23	2.2868171849737816E-4	0.0	0.0	27.01634388242101	0.0
24	2.2868171849737816E-4	0.0	0.0	27.55534669291933	0.0
25	2.2868171849737816E-4	0.0	0.0	28.086688665847987	0.0
26	2.2868171849737816E-4	0.0	0.0	28.54256567167251	0.0
27	2.2868171849737816E-4	0.0	0.0	29.01765194185081	0.0
28	2.2868171849737816E-4	0.0	0.0	29.468269268149896	0.0
29	2.2868171849737816E-4	0.0	0.0	30.15248496989405	0.0
30	2.2868171849737816E-4	0.0	0.0	30.76500895288928	0.0
31	2.2868171849737816E-4	0.0	0.0	31.25930448742136	0.0
32	3.4302257774606725E-4	0.0	0.0	31.742165936028577	0.0
33	3.4302257774606725E-4	0.0	0.0	32.19975805474183	0.0
34	3.4302257774606725E-4	0.0	0.0	32.90569851974324	0.0
35	3.4302257774606725E-4	0.0	0.0	33.386273151165476	0.0
36	3.4302257774606725E-4	0.0	0.0	33.89177408990393	0.0
37	3.4302257774606725E-4	0.0	0.0	34.3564553418906	0.0
38	3.4302257774606725E-4	0.0	0.0	34.7756289318963	0.0
39	3.4302257774606725E-4	0.0	0.0	35.29119186624864	0.0
40	3.4302257774606725E-4	0.0	0.0	35.81178579840792	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	75	0.0	46.000004	5
AAGTACG	35	1.01919795E-7	46.000004	19
CGTTTTA	35	1.01919795E-7	46.000004	46
AGTACGT	35	1.01919795E-7	46.000004	20
GTACGTC	35	1.01919795E-7	46.000004	21
TACGTCA	35	1.01919795E-7	46.000004	22
ACAACGA	45	3.092282E-10	46.0	12
CTGTCGA	20	6.3106226E-4	46.0	11
ATCTCGC	25	3.416482E-5	46.0	13
CCGGGTA	50	1.6370905E-11	46.0	4
GTACCGA	20	6.3106226E-4	46.0	7
CTGTACG	25	3.416482E-5	46.0	34
TTAGCTA	20	6.3106226E-4	46.0	33
CGACGGT	20	6.3106226E-4	46.0	27
GGGTACG	25	3.416482E-5	46.0	6
TAGCGAG	25	3.416482E-5	46.0	26
CGACATA	20	6.3106226E-4	46.0	41
CGATTGA	25	3.416482E-5	46.0	9
GCGTTTC	30	1.8608898E-6	46.0	12
CGACAAA	20	6.3106226E-4	46.0	18
>>END_MODULE