FastQCFastQC Report
Thu 26 May 2016
SRR1544985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544985_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences474369
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT27350.5765553819916563Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT18120.38198111596668416Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTT17610.371229991841794Illumina Single End Adapter 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTTG11190.2358923116814126Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.1933094278926321No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC7260.15304541401314167No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT7100.14967251232690162Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC6120.1290134894986814No Hit
GCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC5850.12332171790315133No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA5650.11910559079535131No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC5400.11383543191060125No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA5190.1094084984474112No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4890.10308430778571112No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT4870.10266269507493112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG750.046.0000041
GTGACGG351.0178701E-746.0000041
AGCGTGC206.307689E-446.046
AAGTAGG405.5988494E-946.01
ACCGGGA551.8189894E-1246.03
TTTACGG301.8590745E-646.01
CACGGGT253.4141E-546.03
CAACGCA1150.046.016
AACTTCG206.307689E-446.010
CGCATGT206.307689E-446.038
TGTATCG206.307689E-446.011
TCGATCA206.307689E-446.016
ACGGGTG501.6370905E-1146.04
ACGGGTC600.046.04
CGTTCAC206.307689E-446.020
TCTACGG253.4141E-546.01
CCTCATG206.307689E-446.019
CAAGGAT206.307689E-446.03
TCGTCGG206.307689E-446.01
GCGATCT206.307689E-446.08
Produced by FastQC (version 0.11.5)