FastQCFastQC Report
Thu 26 May 2016
SRR1544984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544984_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2445629
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT84840.3469046204473369Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT66180.27060523080156473Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT64400.26332693961349Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49540.20256547497596733No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC39800.16273931982324383No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT38010.15542013935883162No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31080.1270838708569452No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA28840.11792467295734553No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26540.10852013939972088No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA24620.10066939834292118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG253.4182944E-546.025
TATAGCG206.312857E-446.01
TGTCGAT253.4182944E-546.018
TACGTCG301.8622704E-646.03
CGAATAT2650.043.3962314
ACGTAGG1600.043.1251
CATGCGG4450.042.898881
TCGTTGA5750.042.423
ATCAACG21100.042.2938414
TTATGAC20800.041.46634726
CGCACTT18850.041.2413834
CTAACGG950.041.1578941
CACTTAC18950.041.0237536
CAACGCA21700.041.01843616
TTAATCG6000.041.01666619
TAATCGT6000.041.01666620
CATACGA12300.040.9512217
CTTATGA21250.040.80470325
AACGCAA21600.040.7824117
TCAACGC22000.040.77272815
Produced by FastQC (version 0.11.5)