FastQCFastQC Report
Thu 26 May 2016
SRR1544983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544983_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1347763
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA59330.440210927292113No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT59090.43843019878123973Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT42700.31682128089285727Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT40940.30376260514645376Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30430.2257815357744648No Hit
TGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTTG27380.20315144428211784Illumina Single End Adapter 2 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21780.16160111236174313No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16410.12175731193095522No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT15770.11700870256862667No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14300.10610174043952832No Hit
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC13480.10001758469404488Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG453.110472E-1046.01
AATACGG750.046.01
CTTACGA206.311845E-446.027
GGCGATA1600.043.1257
ATTGCGG3000.042.933331
GCGATAG700.042.7142838
TCATCGA1350.042.59259415
TTAATCG3300.042.51515219
GCGGGAT5300.042.094344
CGGGATA1150.042.0000045
CGTTTTT20750.041.8987961
TAATCGT3350.041.88059620
CATACGA5500.041.8181817
ACGGGAT3200.041.68754
CATGCGG3100.041.548391
ATCGAGG1950.041.282051
CGTTGAT3400.041.26470624
TACGGGC2300.041.0000043
CGCACTT9350.040.83422534
CCGCACT9350.040.83422533
Produced by FastQC (version 0.11.5)