FastQCFastQC Report
Thu 26 May 2016
SRR1544982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544982_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1381211
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT61310.44388583641456664Illumina PCR Primer Index 7 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT57060.4131157368425244TruSeq Adapter, Index 23 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT54680.3958844810821808Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.2308119469074602No Hit
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT24980.18085578524932108Illumina PCR Primer Index 7 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC24020.1739053627577539TruSeq Adapter, Index 23 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC18490.13386803319695545Illumina PCR Primer Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC16800.12163239360242571TruSeq Adapter, Index 23 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT16440.119025985168088TruSeq Adapter, Index 23 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC16130.1167815779051861TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA206.3118996E-446.037
CGAACGG206.3118996E-446.01
TTTACGG501.6370905E-1146.01
ATAGCGG1900.046.01
TATATCG253.417516E-546.045
TAAGTCG206.3118996E-446.032
TAACCGG750.046.01
ACCTACG206.3118996E-446.029
GTTCGTG301.8616774E-646.012
ACTACGG950.046.01
ACGCTCG253.417516E-546.018
GCGATCG206.3118996E-446.08
TTAACGA206.3118996E-446.039
TTCGGGT501.6370905E-1146.03
ATCGGTT253.417516E-546.032
CGACTAC206.3118996E-446.045
TTACCGA206.3118996E-446.015
GCGTCTA206.3118996E-446.027
CCGTTTA206.3118996E-446.013
ATACCGG405.6134013E-946.01
Produced by FastQC (version 0.11.5)