##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544982_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1381211
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90301988617235	34.0	33.0	34.0	31.0	34.0
2	33.20105110660138	34.0	33.0	34.0	31.0	34.0
3	33.25668344662763	34.0	34.0	34.0	31.0	34.0
4	36.55333471859115	37.0	37.0	37.0	35.0	37.0
5	36.54254635968002	37.0	37.0	37.0	35.0	37.0
6	36.66074119015849	37.0	37.0	37.0	35.0	37.0
7	36.5084024091902	37.0	37.0	37.0	35.0	37.0
8	36.52794395642664	37.0	37.0	37.0	35.0	37.0
9	38.30538563622792	39.0	39.0	39.0	37.0	39.0
10	38.19255059509373	39.0	39.0	39.0	35.0	39.0
11	37.74789804019806	39.0	37.0	39.0	35.0	39.0
12	37.70201439171857	39.0	37.0	39.0	35.0	39.0
13	37.68778412566943	39.0	37.0	39.0	35.0	39.0
14	39.09147407601011	41.0	38.0	41.0	35.0	41.0
15	39.13739537261142	41.0	38.0	41.0	35.0	41.0
16	39.14205722369718	41.0	38.0	41.0	35.0	41.0
17	39.172491386182124	41.0	38.0	41.0	35.0	41.0
18	39.15772391039457	41.0	38.0	41.0	35.0	41.0
19	39.10445181800608	41.0	38.0	41.0	35.0	41.0
20	39.030062025280714	41.0	38.0	41.0	35.0	41.0
21	38.98781938458353	41.0	38.0	41.0	35.0	41.0
22	38.96072070089219	41.0	37.0	41.0	35.0	41.0
23	38.95483094183293	41.0	37.0	41.0	35.0	41.0
24	38.9138067970788	41.0	37.0	41.0	35.0	41.0
25	38.8800972479947	41.0	37.0	41.0	35.0	41.0
26	38.83430771981978	41.0	37.0	41.0	35.0	41.0
27	38.81400597012332	41.0	37.0	41.0	35.0	41.0
28	38.80759492937719	41.0	37.0	41.0	35.0	41.0
29	38.79329009108673	41.0	37.0	41.0	35.0	41.0
30	38.72373446200472	41.0	37.0	41.0	35.0	41.0
31	38.6842068300933	41.0	37.0	41.0	35.0	41.0
32	38.55426288959471	40.0	36.0	41.0	35.0	41.0
33	38.50396934284479	40.0	36.0	41.0	35.0	41.0
34	38.39281398714606	40.0	36.0	41.0	35.0	41.0
35	38.337116486908954	40.0	36.0	41.0	35.0	41.0
36	38.31113566283501	40.0	36.0	41.0	35.0	41.0
37	38.28924979601234	40.0	36.0	41.0	35.0	41.0
38	38.24259291303067	40.0	36.0	41.0	35.0	41.0
39	38.20246291116998	40.0	36.0	41.0	35.0	41.0
40	38.122663372938675	40.0	36.0	41.0	35.0	41.0
41	38.09837671434705	40.0	35.0	41.0	35.0	41.0
42	37.9795288337553	40.0	35.0	41.0	35.0	41.0
43	37.87647289226628	40.0	35.0	41.0	35.0	41.0
44	37.804398459033415	40.0	35.0	41.0	35.0	41.0
45	37.755307480175006	40.0	35.0	41.0	35.0	41.0
46	37.8015053456713	40.0	35.0	41.0	35.0	41.0
47	37.74434536070159	40.0	35.0	41.0	35.0	41.0
48	37.767233246766786	40.0	35.0	41.0	35.0	41.0
49	37.71761809021214	40.0	35.0	41.0	35.0	41.0
50	37.644611141961654	40.0	35.0	41.0	35.0	41.0
51	37.585581058940306	40.0	35.0	41.0	34.0	41.0
52	37.37189176744176	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	4.0
14	9.0
15	14.0
16	22.0
17	48.0
18	110.0
19	179.0
20	286.0
21	477.0
22	785.0
23	1286.0
24	2379.0
25	4004.0
26	5773.0
27	6790.0
28	7133.0
29	7725.0
30	9035.0
31	10937.0
32	14263.0
33	19798.0
34	50461.0
35	194551.0
36	47275.0
37	72102.0
38	139979.0
39	780312.0
40	5469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.831776607629102	23.350306361591386	26.11787771745229	18.700039313327217
2	26.619176939656576	23.674731811432142	27.297060333287238	22.409030915624044
3	25.683114310557908	25.0737215385629	26.647268230559995	22.595895920319197
4	26.307493931050363	27.211700457062676	23.549262205412496	22.931543406474464
5	23.087638311597576	35.891330144344344	21.17757533063377	19.843456213424307
6	86.78674004189078	2.2812589821540663	8.150601175345404	2.781399800609755
7	87.3413258365304	2.0270617595718536	5.633679430586637	4.9979329733111015
8	74.91056761059679	4.55773954884518	12.620157238828824	7.911535601729207
9	41.316786501121115	17.811760838858074	17.1260582199244	23.74539444009641
10	33.10457272639734	20.183447713636802	29.909839988242204	16.802139571723654
11	32.18661015587047	17.758691467125587	28.69843926814947	21.356259108854474
12	20.922147304068677	26.89364622783919	32.04072368378184	20.14348278431029
13	15.914729900065957	29.539295589160524	32.91611491654787	21.629859594225646
14	15.387873395158309	24.173497025436376	39.795078376873626	20.64355120253169
15	17.594198134825163	19.72471982919337	40.665473993473846	22.015608042507626
16	17.73957780527378	23.650694933648804	28.486306581688098	30.12342067938932
17	19.472115411765472	23.08191869308889	33.46121628049588	23.984749614649754
18	22.016983646959083	27.89385546451628	29.05877523419666	21.030385654327976
19	23.580394306155974	23.665030180037665	27.07211280535704	25.68246270844932
20	19.81123810916652	30.270031153820813	27.927159572288375	21.991571164724288
21	19.548135657766988	23.521170914509078	29.085852921820056	27.84484050590388
22	18.14357111259612	27.79488434424574	25.950488375780385	28.111056167377757
23	18.168621593659477	22.652006101891743	35.71713518064944	23.46223712379933
24	19.880018331739322	22.67604297967508	32.421548916132295	25.0223897724533
25	16.97322132534421	28.5753588698613	30.27328916436374	24.178130640430755
26	17.882061466350905	27.875321004538772	28.288726342318444	25.953891186791882
27	20.281912032267336	27.78757192058274	31.92104609650517	20.00946995064476
28	18.889365925988137	25.571183548349964	29.194453273250794	26.344997252411105
29	19.492677078303025	28.84389133883237	27.958653674203287	23.704777908661313
30	23.44449906639898	27.29879793891013	24.741404463184843	24.515298531506048
31	25.93296751908289	26.313213549559045	25.515073366777415	22.238745564580647
32	20.54269767616968	28.385887456731812	28.275911500849617	22.795503366248894
33	18.550098428118513	27.31284358436184	26.969087271966412	27.167970715553235
34	18.980879822127108	25.06351310552841	32.71665227108675	23.238954801257737
35	23.482798790336886	23.8187358774293	30.1511499691213	22.547315363112517
36	20.223847044369037	25.065395511619876	28.741083006144606	25.96967443786648
37	19.025985168088003	25.555038296103927	31.884701178893017	23.534275356915053
38	17.826240885715507	23.972948376460945	30.364513459565558	27.836297278257994
39	22.628765626685567	22.380143222143467	30.06875850250251	24.92233264866845
40	16.160311494768	22.163376920687718	32.135567990698014	29.54074359384627
41	20.54110487101536	25.86056728479573	26.37960456440037	27.21872327978853
42	20.78292165353447	21.89549605382523	30.759384337367717	26.56219795527258
43	23.01994409253908	23.978667994969634	28.73796979607026	24.263418116421025
44	17.917754781854477	26.966770464469224	29.878780287733008	25.236694465943295
45	20.683805732795353	25.03469781228212	29.219069352908427	25.0624271020141
46	22.43002698356732	22.04702974418825	30.057536466188004	25.46540680605642
47	19.314138100550892	20.431490916304604	34.16559815987564	26.088772823268858
48	20.298491685919096	19.37509909782068	29.6397871143511	30.686622101909123
49	19.66969565113513	22.106108335366574	33.1363564292494	25.087839584248893
50	18.818630897089584	22.473467124139614	31.90287363769909	26.80502834107171
51	18.80878446522653	20.243539908095144	31.908303655270633	29.0393719714077
52	19.246805882663836	19.540750833869698	36.73913688784697	24.473306395619495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	170.0
1	387.0
2	604.0
3	3409.5
4	6215.0
5	4092.0
6	1969.0
7	2127.5
8	2286.0
9	2288.5
10	2291.0
11	2227.5
12	2164.0
13	2106.0
14	1960.5
15	1873.0
16	1680.5
17	1488.0
18	1549.5
19	1611.0
20	1786.0
21	1961.0
22	2130.0
23	2299.0
24	3049.0
25	3799.0
26	4697.0
27	5595.0
28	6708.5
29	7822.0
30	9156.0
31	10490.0
32	12641.0
33	14792.0
34	17135.0
35	19478.0
36	22090.0
37	24702.0
38	29167.0
39	39949.0
40	46266.0
41	62011.0
42	77756.0
43	104967.0
44	132178.0
45	159116.0
46	186054.0
47	188287.0
48	190520.0
49	170027.5
50	149535.0
51	128590.5
52	107646.0
53	93837.0
54	80028.0
55	69792.5
56	59557.0
57	54618.0
58	49679.0
59	48037.0
60	46395.0
61	41629.0
62	36863.0
63	32030.5
64	22137.5
65	17077.0
66	13936.0
67	10795.0
68	8458.5
69	6122.0
70	5277.0
71	4432.0
72	3572.0
73	2712.0
74	2195.0
75	1678.0
76	1252.0
77	826.0
78	603.5
79	381.0
80	264.0
81	147.0
82	94.0
83	41.0
84	34.5
85	28.0
86	16.5
87	5.0
88	4.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1381211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.915951615293107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.0352160829903	16.702462004445046
2	13.678596067068357	6.816304755444276
3	5.8282972629573795	4.356527178101739
4	2.93170018135697	2.9218439947694517
5	1.6312202403880711	2.0321702291697985
6	1.0764892756712596	1.6093052824204215
7	0.7024227411299181	1.2251071722032219
8	0.527236421850676	1.0509277741321366
9	0.3994029682688163	0.8956354529151248
>10	4.6915640928265905	29.69539411720758
>50	1.301838043845081	20.900550156043167
>100	0.18318219999624338	7.640532703233512
>500	0.008750742034396285	1.4819707436677392
>1k	0.0032086054126119713	1.413581038906259
>5k	8.750742034396284E-4	1.2576873973405045
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	6131	0.44388583641456664	Illumina PCR Primer Index 7 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT	5706	0.4131157368425244	TruSeq Adapter, Index 23 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	5468	0.3958844810821808	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3188	0.2308119469074602	No Hit
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	2498	0.18085578524932108	Illumina PCR Primer Index 7 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2402	0.1739053627577539	TruSeq Adapter, Index 23 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1849	0.13386803319695545	Illumina PCR Primer Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1680	0.12163239360242571	TruSeq Adapter, Index 23 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	1644	0.119025985168088	TruSeq Adapter, Index 23 (96% over 26bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1613	0.1167815779051861	TruSeq Adapter, Index 23 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42071776144267603	0.0
2	0.0	0.0	0.0	1.581221116831534	0.0
3	0.0	0.0	0.0	2.5693395143826687	0.0
4	0.0	0.0	0.0	3.1342785425253634	0.0
5	0.0	0.0	0.0	6.412995552453608	0.0
6	0.0	0.0	0.0	6.967870948030388	0.0
7	0.0	0.0	0.0	9.531925245310093	0.0
8	0.0	0.0	0.0	12.306881425068292	0.0
9	0.0	0.0	0.0	14.799114689935136	0.0
10	0.0	0.0	0.0	16.1839863713799	0.0
11	0.0	0.0	0.0	17.479009362074297	0.0
12	0.0	0.0	0.0	18.44721769519646	0.0
13	0.0	0.0	0.0	18.849980198535924	0.0
14	7.240023428715815E-5	0.0	0.0	19.160142802222108	0.0
15	7.240023428715815E-5	0.0	0.0	20.33201299439405	0.0
16	7.240023428715815E-5	0.0	0.0	21.61733435369397	0.0
17	7.240023428715815E-5	0.0	0.0	23.242574812972094	0.0
18	7.240023428715815E-5	0.0	0.0	24.036443381930784	0.0
19	7.240023428715815E-5	0.0	0.0	24.948686333948977	0.0
20	7.240023428715815E-5	0.0	0.0	26.09680924927473	0.0
21	7.240023428715815E-5	0.0	0.0	27.081380035345795	0.0
22	7.240023428715815E-5	0.0	0.0	28.136903051018272	0.0
23	7.240023428715815E-5	0.0	0.0	28.880236256444526	0.0
24	7.240023428715815E-5	0.0	0.0	29.579622519658475	0.0
25	7.240023428715815E-5	0.0	0.0	30.24874548494039	0.0
26	7.240023428715815E-5	0.0	0.0	30.893107570096095	0.0
27	7.240023428715815E-5	0.0	0.0	31.530664033228813	0.0
28	7.240023428715815E-5	0.0	0.0	32.151713242944055	0.0
29	7.240023428715815E-5	0.0	0.0	32.96093066157162	0.0
30	7.240023428715815E-5	0.0	0.0	33.67276976508296	0.0
31	7.240023428715815E-5	0.0	0.0	34.30308620478696	0.0
32	7.240023428715815E-5	0.0	0.0	34.937746658548186	0.0
33	7.240023428715815E-5	0.0	0.0	35.546849829606046	0.0
34	7.240023428715815E-5	0.0	0.0	36.25398291788872	0.0
35	7.240023428715815E-5	0.0	0.0	36.83456039663744	0.0
36	1.448004685743163E-4	0.0	0.0	37.42686671334069	0.0
37	1.448004685743163E-4	0.0	0.0	38.03835909213002	0.0
38	1.448004685743163E-4	0.0	0.0	38.60966934088999	0.0
39	1.448004685743163E-4	0.0	0.0	39.26742546938882	0.0
40	1.448004685743163E-4	0.0	0.0	39.883623863406825	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	20	6.3118996E-4	46.0	37
CGAACGG	20	6.3118996E-4	46.0	1
TTTACGG	50	1.6370905E-11	46.0	1
ATAGCGG	190	0.0	46.0	1
TATATCG	25	3.417516E-5	46.0	45
TAAGTCG	20	6.3118996E-4	46.0	32
TAACCGG	75	0.0	46.0	1
ACCTACG	20	6.3118996E-4	46.0	29
GTTCGTG	30	1.8616774E-6	46.0	12
ACTACGG	95	0.0	46.0	1
ACGCTCG	25	3.417516E-5	46.0	18
GCGATCG	20	6.3118996E-4	46.0	8
TTAACGA	20	6.3118996E-4	46.0	39
TTCGGGT	50	1.6370905E-11	46.0	3
ATCGGTT	25	3.417516E-5	46.0	32
CGACTAC	20	6.3118996E-4	46.0	45
TTACCGA	20	6.3118996E-4	46.0	15
GCGTCTA	20	6.3118996E-4	46.0	27
CCGTTTA	20	6.3118996E-4	46.0	13
ATACCGG	40	5.6134013E-9	46.0	1
>>END_MODULE