FastQCFastQC Report
Thu 26 May 2016
SRR1544981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544981_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences810593
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT48990.60437235456018Illumina PCR Primer Index 7 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT41050.5064193744579585TruSeq Adapter, Index 23 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT35740.44091177693367695Illumina PCR Primer Index 7 (95% over 24bp)
TGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTTG24950.3077993518325473Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.2477198791502024No Hit
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT15010.1851730769942499Illumina PCR Primer Index 7 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC14720.1815954492575189TruSeq Adapter, Index 23 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA14620.18036178452071508No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC11880.14655937073229106Illumina PCR Primer Index 7 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC10480.12928806441703788TruSeq Adapter, Index 23 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC10410.12842449910127524TruSeq Adapter, Index 23 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT10170.12546370373294613TruSeq Adapter, Index 23 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG206.310348E-446.019
TACCCCG206.310348E-446.024
GCGACGG800.046.01
CGACGGT253.4162586E-546.027
CTATCGA206.310348E-446.013
GCGCGAC253.4162586E-546.08
GATCCGG405.6079443E-946.01
CGCGAAG206.310348E-446.031
AGGCGAT206.310348E-446.05
TTATCGG206.310348E-446.01
ACGGGTG551.8189894E-1246.04
TACGCGG1300.046.01
GAAACGG206.310348E-446.01
CTACCGT206.310348E-446.029
GCGATAG551.8189894E-1246.08
TTACGGC206.310348E-446.02
ACCGTTC206.310348E-446.031
CGGTCTA253.4162586E-546.030
TCGAGCG206.310348E-446.031
CGCAATA253.4162586E-546.036
Produced by FastQC (version 0.11.5)