##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544981_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	810593
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9143195166995	34.0	33.0	34.0	31.0	34.0
2	33.26518240349966	34.0	33.0	34.0	31.0	34.0
3	33.38530063792803	34.0	34.0	34.0	31.0	34.0
4	36.616636215708745	37.0	37.0	37.0	35.0	37.0
5	36.61343238838727	37.0	37.0	37.0	35.0	37.0
6	36.4243300892063	37.0	37.0	37.0	35.0	37.0
7	36.285056742409566	37.0	37.0	37.0	35.0	37.0
8	36.402377025214875	37.0	37.0	37.0	35.0	37.0
9	38.27325797286678	39.0	39.0	39.0	37.0	39.0
10	38.2255496901651	39.0	39.0	39.0	35.0	39.0
11	37.83875014958185	39.0	37.0	39.0	35.0	39.0
12	37.84067466657126	39.0	38.0	39.0	35.0	39.0
13	37.839898691451815	39.0	38.0	39.0	35.0	39.0
14	39.18304993998221	41.0	39.0	41.0	35.0	41.0
15	39.1992331539996	41.0	39.0	41.0	35.0	41.0
16	39.23127019354966	41.0	39.0	41.0	35.0	41.0
17	39.28968791983153	41.0	39.0	41.0	35.0	41.0
18	39.290260340269406	41.0	39.0	41.0	35.0	41.0
19	39.27043904894318	41.0	39.0	41.0	35.0	41.0
20	39.20782316156197	41.0	38.0	41.0	35.0	41.0
21	39.16939450501053	41.0	38.0	41.0	35.0	41.0
22	39.14440539210183	41.0	38.0	41.0	35.0	41.0
23	39.11653690569744	41.0	38.0	41.0	35.0	41.0
24	39.09126281623453	41.0	38.0	41.0	35.0	41.0
25	39.0517682733505	41.0	38.0	41.0	35.0	41.0
26	38.99918701493845	41.0	38.0	41.0	35.0	41.0
27	39.05999805080972	41.0	38.0	41.0	35.0	41.0
28	39.060596378207066	41.0	38.0	41.0	35.0	41.0
29	39.04178422463554	41.0	38.0	41.0	35.0	41.0
30	38.99685168759168	41.0	38.0	41.0	35.0	41.0
31	38.95435810573247	41.0	38.0	41.0	35.0	41.0
32	38.857688136956526	41.0	38.0	41.0	35.0	41.0
33	38.80195733247141	41.0	38.0	41.0	35.0	41.0
34	38.6852637513524	41.0	37.0	41.0	35.0	41.0
35	38.62648702863213	41.0	37.0	41.0	35.0	41.0
36	38.59843472618194	41.0	37.0	41.0	35.0	41.0
37	38.58372574152503	41.0	37.0	41.0	35.0	41.0
38	38.55614839999852	41.0	37.0	41.0	35.0	41.0
39	38.53139738438402	41.0	37.0	41.0	35.0	41.0
40	38.4787297694404	40.0	37.0	41.0	35.0	41.0
41	38.44975591943182	40.0	36.0	41.0	35.0	41.0
42	38.38023521051872	40.0	36.0	41.0	35.0	41.0
43	38.30614994207944	40.0	36.0	41.0	35.0	41.0
44	38.09616169890438	40.0	35.0	41.0	35.0	41.0
45	38.12110269888834	40.0	35.0	41.0	35.0	41.0
46	38.09992561001637	40.0	35.0	41.0	35.0	41.0
47	38.02804983512071	40.0	35.0	41.0	35.0	41.0
48	37.99537869189593	40.0	36.0	41.0	35.0	41.0
49	37.960547401717015	40.0	35.0	41.0	35.0	41.0
50	37.89855574869262	40.0	35.0	41.0	35.0	41.0
51	37.83227711070784	40.0	35.0	41.0	35.0	41.0
52	37.37779378800458	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	6.0
15	8.0
16	8.0
17	25.0
18	60.0
19	76.0
20	148.0
21	233.0
22	352.0
23	634.0
24	1187.0
25	2094.0
26	3202.0
27	3706.0
28	3606.0
29	3495.0
30	3935.0
31	4728.0
32	6226.0
33	9075.0
34	26522.0
35	107756.0
36	24753.0
37	40572.0
38	81503.0
39	483871.0
40	2811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.142015783506643	22.96491580855004	25.404611192053224	20.488457215890097
2	26.56289901343831	24.09310221035711	27.01762783542419	22.32637094078039
3	25.53019826225985	24.911515396752748	26.69823203506569	22.860054305921715
4	26.21920001776477	26.80741136427282	23.987500508886704	22.9858881090757
5	23.067803447599474	35.187695921381014	21.537195608647004	20.20730502237251
6	85.27892542866765	2.2754946070346027	8.968495903616242	3.477084060681501
7	85.8256856400191	1.9140308391510905	6.558285107322664	5.701998413507148
8	72.90243068963093	4.566656756226614	13.872436598885013	8.658475955257448
9	40.83491962057407	17.297830107094438	17.700374910713514	24.166875361617976
10	32.27439664541885	19.607250494391142	31.223314289662014	16.895038570527994
11	31.343473235026703	17.657936843767462	28.964720889521622	22.033869031684212
12	20.48426275578496	25.890798464827604	32.984617434396796	20.640321344990642
13	15.787084270404506	28.52652317500891	33.365696471595484	22.320696082991095
14	15.237980096053136	23.889177429363443	39.331575772304966	21.541266702278456
15	17.353468386724288	19.49251967386839	40.572519131055905	22.581492808351417
16	17.332496086198624	24.10371172709362	28.880584954471605	29.683207232236153
17	18.949213723779998	23.003406148338314	33.720128350479214	24.32725177740247
18	21.331420330548127	28.33888276854106	29.318412569563268	21.011284331347543
19	23.071134342388845	23.559172112268424	27.269542174679522	26.100151370663205
20	19.328318897399804	30.793505495359568	27.77719521387429	22.10098039336634
21	19.158443263141923	23.542641004795257	29.02011243620411	28.27880329585871
22	17.85507646870871	28.07709911139129	25.747569988884685	28.320254431015318
23	17.846317449077404	22.655142593138603	35.59973994347348	23.898800014310513
24	19.946261564064827	22.95936431723442	31.77957371948684	25.31480039921391
25	16.935996239789883	28.683692062477718	30.161622417168665	24.218689280563733
26	18.078123053122837	27.186763270839993	28.233897899439054	26.50121577659812
27	20.70101765004139	27.420049272569585	31.768840836276652	20.11009224111237
28	19.101694685248948	25.232885060690137	28.896622596050054	26.76879765801086
29	19.737895590018663	28.54367111485049	27.894393363870645	23.824039931260202
30	23.081620492651673	27.117307946157936	24.435074075399122	25.365997485791265
31	25.88055904751213	25.974194201035537	25.257311622478852	22.88793512897348
32	19.972661989432428	28.215516294860677	28.71786457568718	23.093957140019715
33	18.415530358638676	27.11200318778968	26.69921896685513	27.773247486716514
34	19.338434948241595	24.69130624123327	33.15116217386531	22.81909663665983
35	23.089886046388262	23.471335183007998	30.58600308662917	22.85277568397457
36	20.190033716057258	24.698954962601455	28.673576011635927	26.437435309705364
37	18.945019263674865	24.8439105691759	32.751948265035594	23.45912190211364
38	17.753669227343437	23.38288142137916	30.350249755425967	28.51319959585143
39	22.581369441877737	21.801693328217738	30.471025533159057	25.145911696745472
40	16.142256348130317	21.508204487332115	32.17928109421128	30.170258070326295
41	20.616634982044012	25.640734622677474	26.240048951816757	27.50258144346176
42	20.589124258413285	21.31562942191704	31.066392135140568	27.028854184529106
43	23.616290789582443	23.469854785323832	28.417097112854417	24.49675731223931
44	17.965612829126332	26.107306626136666	30.532955502946606	25.394125041790396
45	20.606025465307496	24.367962713716995	29.552808869555992	25.473202951419516
46	22.58593400140391	21.124904853607173	30.358515309162552	25.930645835826365
47	19.18792785035153	19.65190915786344	34.67942604981785	26.480736941967177
48	20.207551755319873	18.586022825264962	29.54812094355614	31.658304475859033
49	19.554079544234902	21.01288809550539	33.55901173585264	25.874020624407066
50	19.094786162722848	21.469714147543836	32.29845310778652	27.137046581946795
51	18.691377793788007	19.341025644188882	32.30684202799679	29.660754534026324
52	19.052471462250477	18.955382047464017	37.30565154152577	24.686494948759734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	223.5
2	369.0
3	2145.0
4	3921.0
5	2653.0
6	1385.0
7	1401.5
8	1418.0
9	1447.5
10	1477.0
11	1434.5
12	1392.0
13	1374.5
14	1272.5
15	1188.0
16	1072.5
17	957.0
18	940.5
19	924.0
20	1021.5
21	1119.0
22	1208.0
23	1297.0
24	1659.5
25	2022.0
26	2483.5
27	2945.0
28	3419.5
29	3894.0
30	4659.0
31	5424.0
32	6613.5
33	7803.0
34	9006.0
35	10209.0
36	11608.5
37	13008.0
38	15385.0
39	21174.0
40	24586.0
41	33605.0
42	42624.0
43	58999.5
44	75375.0
45	94174.0
46	112973.0
47	114630.5
48	116288.0
49	103797.0
50	91306.0
51	78202.0
52	65098.0
53	56238.5
54	47379.0
55	41040.5
56	34702.0
57	31810.0
58	28918.0
59	27860.0
60	26802.0
61	24044.0
62	21286.0
63	19225.0
64	13566.0
65	9968.0
66	8150.5
67	6333.0
68	5003.5
69	3674.0
70	3135.5
71	2597.0
72	2131.0
73	1665.0
74	1352.0
75	1039.0
76	765.0
77	491.0
78	365.5
79	240.0
80	160.5
81	81.0
82	55.5
83	30.0
84	24.0
85	18.0
86	10.5
87	3.0
88	2.5
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	810593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.566421347154826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28833558689979	21.48462881339396
2	12.703726600102211	7.766149198755656
3	4.7149369570944755	4.323562489674654
4	2.2083968741176596	2.7001115742408004
5	1.2319521445800334	1.882818416538215
6	0.8480264603876285	1.555268046104673
7	0.6011508444588277	1.2862521003448524
8	0.47282580326445	1.1562074181110489
9	0.4274270525520437	1.175842384513043
>10	6.137857814116067	41.984406609154746
>50	0.25883315792270123	5.377785472769792
>100	0.09640421168056232	5.144894592447325
>500	0.005265776268266008	0.9664784903653997
>1k	0.004860716555322469	3.1955943935858273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	4899	0.60437235456018	Illumina PCR Primer Index 7 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT	4105	0.5064193744579585	TruSeq Adapter, Index 23 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	3574	0.44091177693367695	Illumina PCR Primer Index 7 (95% over 24bp)
TGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTTG	2495	0.3077993518325473	Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2008	0.2477198791502024	No Hit
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	1501	0.1851730769942499	Illumina PCR Primer Index 7 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1472	0.1815954492575189	TruSeq Adapter, Index 23 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1462	0.18036178452071508	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1188	0.14655937073229106	Illumina PCR Primer Index 7 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1048	0.12928806441703788	TruSeq Adapter, Index 23 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1041	0.12842449910127524	TruSeq Adapter, Index 23 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	1017	0.12546370373294613	TruSeq Adapter, Index 23 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.516288692352389	0.0
2	0.0	0.0	0.0	1.9015708253093724	0.0
3	0.0	0.0	0.0	3.000025906959473	0.0
4	0.0	0.0	0.0	3.6484400926235483	0.0
5	0.0	0.0	0.0	7.258636578406179	0.0
6	0.0	0.0	0.0	7.867203393071492	0.0
7	0.0	0.0	0.0	10.76483512687625	0.0
8	0.0	0.0	0.0	13.794962453413735	0.0
9	0.0	0.0	0.0	16.652006617377648	0.0
10	0.0	0.0	0.0	18.255647408748903	0.0
11	0.0	0.0	0.0	19.664986004073562	0.0
12	0.0	0.0	0.0	20.75603909730284	0.0
13	0.0	0.0	0.0	21.20842395628879	0.0
14	0.0	0.0	0.0	21.547805125383515	0.0
15	0.0	0.0	0.0	22.783937191660918	0.0
16	0.0	0.0	0.0	24.173660517670395	0.0
17	0.0	0.0	0.0	25.804195200303976	0.0
18	0.0	0.0	0.0	26.671214777329684	0.0
19	0.0	0.0	0.0	27.610527107932093	0.0
20	0.0	0.0	0.0	28.773256122369673	0.0
21	0.0	0.0	0.0	29.81224856370583	0.0
22	0.0	0.0	0.0	30.89478937025116	0.0
23	0.0	0.0	0.0	31.671381322069152	0.0
24	0.0	0.0	0.0	32.400477181520195	0.0
25	0.0	0.0	0.0	33.077389022604436	0.0
26	0.0	0.0	0.0	33.739373520373356	0.0
27	0.0	0.0	0.0	34.39555979387929	0.0
28	0.0	0.0	0.0	35.028059704438604	0.0
29	0.0	0.0	0.0	35.816124738308865	0.0
30	0.0	0.0	0.0	36.52338473191848	0.0
31	0.0	0.0	0.0	37.140833932688786	0.0
32	0.0	0.0	0.0	37.76136729530109	0.0
33	0.0	0.0	0.0	38.349948741230186	0.0
34	0.0	0.0	0.0	39.04376178920864	0.0
35	0.0	0.0	0.0	39.62173371840122	0.0
36	0.0	0.0	0.0	40.21068526375135	0.0
37	0.0	0.0	0.0	40.81073979173272	0.0
38	0.0	0.0	0.0	41.378348937136145	0.0
39	0.0	0.0	0.0	42.0199779667478	0.0
40	0.0	0.0	0.0	42.60621544967697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	6.310348E-4	46.0	19
TACCCCG	20	6.310348E-4	46.0	24
GCGACGG	80	0.0	46.0	1
CGACGGT	25	3.4162586E-5	46.0	27
CTATCGA	20	6.310348E-4	46.0	13
GCGCGAC	25	3.4162586E-5	46.0	8
GATCCGG	40	5.6079443E-9	46.0	1
CGCGAAG	20	6.310348E-4	46.0	31
AGGCGAT	20	6.310348E-4	46.0	5
TTATCGG	20	6.310348E-4	46.0	1
ACGGGTG	55	1.8189894E-12	46.0	4
TACGCGG	130	0.0	46.0	1
GAAACGG	20	6.310348E-4	46.0	1
CTACCGT	20	6.310348E-4	46.0	29
GCGATAG	55	1.8189894E-12	46.0	8
TTACGGC	20	6.310348E-4	46.0	2
ACCGTTC	20	6.310348E-4	46.0	31
CGGTCTA	25	3.4162586E-5	46.0	30
TCGAGCG	20	6.310348E-4	46.0	31
CGCAATA	25	3.4162586E-5	46.0	36
>>END_MODULE