FastQCFastQC Report
Thu 26 May 2016
SRR1544980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544980_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1303879
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT49290.37802587510037355No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT43860.33638090651049674No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT40000.30677693252211286Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.20400666012720506No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT20010.15346516049418696No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19300.14801986994191946No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC16470.12631540196597996No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT16270.12478151730336941No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC13990.10729523214960898No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC13050.10008597423533931No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAT206.311767E-446.00000414
ATCTACG206.311767E-446.00000428
TATTCGC206.311767E-446.00000423
CGTCGTT206.311767E-446.00000434
GACGCGT206.311767E-446.00000429
GAACCGA206.311767E-446.00000414
CCTTGCG206.311767E-446.00000422
GCCCGTT351.0197073E-746.00000444
CGAAGTT206.311767E-446.00000442
ACGCGTT206.311767E-446.00000430
TCGATGG600.046.01
ACGATTA253.4174096E-546.016
CGGTCTA501.6370905E-1146.030
CGATATC253.4174096E-546.037
ATTACGG1350.044.2962951
ATGACGG1250.044.161
CCATAGG1100.043.9090921
CTAACGG900.043.4444431
CGCACTT6600.043.2121234
CCGCACT6500.043.1692333
Produced by FastQC (version 0.11.5)