##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544980_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1303879
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.917331286108606	34.0	33.0	34.0	31.0	34.0
2	33.21846352307231	34.0	33.0	34.0	31.0	34.0
3	33.27188872587104	34.0	34.0	34.0	31.0	34.0
4	36.55485823454477	37.0	37.0	37.0	35.0	37.0
5	36.54712822278754	37.0	37.0	37.0	35.0	37.0
6	36.636021440639816	37.0	37.0	37.0	35.0	37.0
7	36.433287904782574	37.0	37.0	37.0	35.0	37.0
8	36.47423495585097	37.0	37.0	37.0	35.0	37.0
9	38.29479652636479	39.0	39.0	39.0	37.0	39.0
10	38.13588377449135	39.0	39.0	39.0	35.0	39.0
11	37.51440969599173	39.0	35.0	39.0	35.0	39.0
12	37.415097566568676	39.0	35.0	39.0	35.0	39.0
13	37.409072467613946	39.0	35.0	39.0	35.0	39.0
14	38.73182327501248	41.0	35.0	41.0	35.0	41.0
15	38.79472251642982	41.0	35.0	41.0	35.0	41.0
16	38.800991503045914	41.0	35.0	41.0	35.0	41.0
17	38.87012521867443	41.0	36.0	41.0	35.0	41.0
18	38.84145768127257	41.0	36.0	41.0	35.0	41.0
19	38.76639856919239	41.0	36.0	41.0	35.0	41.0
20	38.6799572659733	41.0	35.0	41.0	35.0	41.0
21	38.62049699396953	41.0	35.0	41.0	35.0	41.0
22	38.60464736375078	41.0	35.0	41.0	35.0	41.0
23	38.5853280864252	41.0	35.0	41.0	35.0	41.0
24	38.52087348595997	40.0	35.0	41.0	35.0	41.0
25	38.47655112169151	40.0	35.0	41.0	35.0	41.0
26	38.41329218432078	40.0	35.0	41.0	35.0	41.0
27	38.39056231444789	40.0	35.0	41.0	35.0	41.0
28	38.4258531658229	40.0	36.0	41.0	35.0	41.0
29	38.44853931998291	40.0	36.0	41.0	35.0	41.0
30	38.37096233622905	40.0	35.0	41.0	35.0	41.0
31	38.315322970919844	40.0	35.0	41.0	35.0	41.0
32	38.251723511154026	40.0	35.0	41.0	35.0	41.0
33	38.1771951231671	40.0	35.0	41.0	35.0	41.0
34	38.08038322574411	40.0	35.0	41.0	35.0	41.0
35	38.01829694319795	40.0	35.0	41.0	35.0	41.0
36	37.9603437128752	40.0	35.0	41.0	35.0	41.0
37	37.90069477305793	40.0	35.0	41.0	35.0	41.0
38	37.843804524806366	40.0	35.0	41.0	35.0	41.0
39	37.73637277692178	40.0	35.0	41.0	35.0	41.0
40	37.64556373712591	40.0	35.0	41.0	34.0	41.0
41	37.631321618033574	40.0	35.0	41.0	35.0	41.0
42	37.54213619515308	40.0	35.0	41.0	35.0	41.0
43	37.46041772281017	39.0	35.0	41.0	34.0	41.0
44	37.38937278689203	39.0	35.0	41.0	34.0	41.0
45	37.34461633326405	39.0	35.0	41.0	34.0	41.0
46	37.372081305090425	39.0	35.0	41.0	34.0	41.0
47	37.31242469584984	39.0	35.0	41.0	34.0	41.0
48	37.32084418876291	39.0	35.0	41.0	34.0	41.0
49	37.24472286155387	39.0	35.0	41.0	34.0	41.0
50	37.16772261843315	39.0	35.0	41.0	34.0	41.0
51	37.104870160498024	39.0	35.0	41.0	34.0	41.0
52	36.89816846501861	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	4.0
13	10.0
14	15.0
15	24.0
16	22.0
17	52.0
18	100.0
19	198.0
20	354.0
21	578.0
22	884.0
23	1416.0
24	2415.0
25	4057.0
26	5917.0
27	6687.0
28	6993.0
29	7616.0
30	8787.0
31	10839.0
32	14144.0
33	20202.0
34	60792.0
35	245793.0
36	47210.0
37	75049.0
38	126941.0
39	650310.0
40	6468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.79287571929604	22.349236393867837	23.590839334017957	19.267048552818167
2	28.693766829590782	24.136518802741666	24.72368985158899	22.446024516078563
3	27.690376177544078	24.713719601282023	24.561404854284792	23.034499366889104
4	27.04921238857287	26.719273797645332	22.04759797496547	24.18391583881633
5	24.637408839317146	35.99582476594838	20.009985589153594	19.356780805580886
6	85.65794832189184	3.5582289460908565	7.819974092688048	2.963848639329263
7	85.13412670961033	3.550329440078412	5.938127694364278	5.377416155946986
8	72.54706916822803	6.432345332657402	12.66106747635325	8.359518022761314
9	48.594846607699026	20.586112668430122	15.545077418993634	15.273963304877217
10	39.834831299530094	20.973878711138074	25.45565961258675	13.735630376745082
11	39.27089860332132	17.142541600869407	24.953082302882397	18.633477492926875
12	22.30084233276247	31.249141983266853	27.99592600233611	18.45408968163457
13	14.943181077385248	34.464547707264245	29.946797210477357	20.64547400487315
14	14.253163061909884	24.246881804216496	42.44703687995589	19.052918253917735
15	15.345979189786782	18.87376052532482	45.48558570235428	20.294674582534117
16	16.728622824663944	21.25803084488668	28.90375563990217	33.10959069054721
17	19.414838340060697	23.58247966260673	32.43682887752621	24.565853119806363
18	23.9095038726753	27.253065660233812	27.55531763300122	21.282112834089666
19	27.524563245515882	22.34402118601496	25.454662587556054	24.676752980913104
20	21.524619999248394	29.88666893170302	27.005266592989074	21.583444476059512
21	20.25740118523268	24.12424772544078	25.579904270258208	30.038446819068334
22	18.9289803731788	29.522448018566138	23.74560829647536	27.8029633117797
23	18.462372658812665	21.94889249692648	36.53858985381312	23.050144990447734
24	19.74638750988397	22.48974022896296	32.7143853072256	25.049486953927474
25	17.6904452023539	28.9045992764666	28.927607546405763	24.477347974773732
26	18.226001032304378	28.987045577081922	27.311199888946753	25.475753501666947
27	20.34161145321	26.967609724521985	33.51967475509614	19.171104067171875
28	17.63223427940783	26.781856291879848	28.976308384443648	26.609601044268675
29	19.051767840420773	29.783055022743675	26.414107444018963	24.75106969281659
30	24.436776725447686	26.206496154934623	22.131501466010267	27.225225653607428
31	25.583278816515946	30.665575563376663	21.625166138882516	22.12597948122487
32	22.390267808592668	28.405703289952516	21.61067092882085	27.593357972633964
33	17.8052564693503	30.172431644347363	24.528503028271796	27.493808858030537
34	17.733547361373255	28.348719474736534	25.940750637137345	27.976982526752863
35	24.4245056481468	26.49149192524766	22.968849103329372	26.115153323276164
36	18.756955208267026	31.888541804876063	25.938143033210903	23.416359953646005
37	19.074929498826197	31.770509380088185	24.373273900415608	24.781287220670016
38	18.59766128605492	28.288591195962205	25.478131022893997	27.635616495088883
39	23.44159235634595	23.939414623596207	28.037877747858502	24.581115272199337
40	15.69739216599086	22.498329983073585	31.868141138863344	29.93613671207221
41	19.62850847356235	27.278221368700624	24.356247780660627	28.7370223770764
42	20.696859141070608	22.624875467738953	27.94638152773378	28.731883863456652
43	21.50866759875725	28.11089065779877	27.209196558883146	23.17124518456084
44	17.229359472773165	30.587654222516047	26.088923895545523	26.094062409165268
45	20.213148612716363	29.1316909007661	25.105933909511542	25.549226577005996
46	21.691276567841033	24.987364625091747	27.262575745141998	26.058783061925222
47	20.193361500568688	21.21400835506976	31.54817279824278	27.044457346118772
48	19.664247986201175	21.012916075801513	28.93006176186594	30.392774176131375
49	19.098014462998485	25.450597793200135	30.024258385939184	25.427129357862192
50	18.199388133408085	25.91751228449879	28.09348106687814	27.789618515214986
51	18.53124408016388	22.64619646454924	30.29023398643586	28.53232546885102
52	20.796254867207768	20.47114801296746	33.63494618749132	25.097650932333444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	138.0
1	402.5
2	667.0
3	3012.0
4	5357.0
5	3605.0
6	1853.0
7	1974.0
8	2095.0
9	2122.0
10	2149.0
11	2113.5
12	2078.0
13	2072.0
14	1914.0
15	1762.0
16	1655.0
17	1548.0
18	1546.5
19	1545.0
20	1914.5
21	2284.0
22	2592.5
23	2901.0
24	3565.0
25	4229.0
26	4750.0
27	5271.0
28	8281.5
29	11292.0
30	11741.0
31	12190.0
32	13138.0
33	14086.0
34	15956.0
35	17826.0
36	19977.5
37	22129.0
38	26449.5
39	36526.5
40	42283.0
41	58177.5
42	74072.0
43	98528.0
44	122984.0
45	144333.0
46	165682.0
47	163165.5
48	160649.0
49	141191.0
50	121733.0
51	106845.5
52	91958.0
53	79719.0
54	67480.0
55	60166.5
56	52853.0
57	51245.0
58	49637.0
59	51708.5
60	53780.0
61	51152.0
62	48524.0
63	45005.5
64	32961.0
65	24435.0
66	19468.0
67	14501.0
68	12145.0
69	9789.0
70	8378.5
71	6968.0
72	5818.5
73	4669.0
74	3666.5
75	2664.0
76	2182.5
77	1701.0
78	1247.0
79	793.0
80	646.5
81	500.0
82	371.5
83	243.0
84	236.0
85	229.0
86	123.5
87	18.0
88	11.5
89	3.5
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1303879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.423994387861505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.63738469110675	14.490629500319004
2	12.916659541929787	5.534528830724431
3	5.276073994936738	3.391037389724002
4	2.758838756687304	2.3642138416113445
5	1.6046068414419536	1.7188543982888296
6	1.0074591116052527	1.2950279011818526
7	0.7037240038423808	1.0553605376245816
8	0.5383292443297414	0.9226530167473684
9	0.40384784613254787	0.7786830590184296
>10	4.945789088282866	27.6283427804235
>50	1.9224409808930816	27.22324681361943
>100	0.2679634811015514	9.39521345642195
>500	0.01077601130434894	1.5729616015565182
>1k	0.006106406405797733	2.629246872738748
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	4929	0.37802587510037355	No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	4386	0.33638090651049674	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	4000	0.30677693252211286	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2660	0.20400666012720506	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	2001	0.15346516049418696	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1930	0.14801986994191946	No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1647	0.12631540196597996	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1627	0.12478151730336941	No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1399	0.10729523214960898	No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1305	0.10008597423533931	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31298916540568567	0.0
2	0.0	0.0	0.0	1.2616968292303197	0.0
3	0.0	0.0	0.0	2.0396064358732673	0.0
4	0.0	0.0	0.0	2.5428739936757934	0.0
5	0.0	0.0	0.0	5.282392001098262	0.0
6	0.0	0.0	0.0	5.790567989821142	0.0
7	0.0	0.0	0.0	8.165788389873601	0.0
8	0.0	0.0	0.0	10.744631978887611	0.0
9	0.0	0.0	0.0	13.048143270963026	0.0
10	0.0	0.0	0.0	14.143183531600709	0.0
11	7.669423313052821E-5	0.0	0.0	15.241138173097351	0.0
12	7.669423313052821E-5	0.0	0.0	16.162235912994994	0.0
13	7.669423313052821E-5	0.0	0.0	16.52484624723613	0.0
14	7.669423313052821E-5	0.0	0.0	16.79749424601516	0.0
15	7.669423313052821E-5	0.0	0.0	17.772047866404783	0.0
16	7.669423313052821E-5	0.0	0.0	18.748825619555188	0.0
17	7.669423313052821E-5	0.0	0.0	20.127327765843305	0.0
18	7.669423313052821E-5	0.0	0.0	20.714728897390017	0.0
19	7.669423313052821E-5	0.0	0.0	21.495859661824447	0.0
20	7.669423313052821E-5	0.0	0.0	22.36012697497237	0.0
21	7.669423313052821E-5	0.0	0.0	23.124691785050608	0.0
22	7.669423313052821E-5	0.0	0.0	23.96894190335146	0.0
23	1.5338846626105642E-4	0.0	0.0	24.576436923978374	0.0
24	1.5338846626105642E-4	0.0	0.0	25.08231208570734	0.0
25	1.5338846626105642E-4	0.0	0.0	25.562111208171924	0.0
26	1.5338846626105642E-4	0.0	0.0	26.025190987814053	0.0
27	1.5338846626105642E-4	0.0	0.0	26.492719032977753	0.0
28	1.5338846626105642E-4	0.0	0.0	26.94391120648465	0.0
29	1.5338846626105642E-4	0.0	0.0	27.555854492633134	0.0
30	1.5338846626105642E-4	0.0	0.0	28.13320867963975	0.0
31	1.5338846626105642E-4	0.0	0.0	28.646293099282985	0.0
32	1.5338846626105642E-4	0.0	0.0	29.122410898557305	0.0
33	1.5338846626105642E-4	0.0	0.0	29.566853979548714	0.0
34	1.5338846626105642E-4	0.0	0.0	30.192678921893826	0.0
35	1.5338846626105642E-4	0.0	0.0	30.674088623254153	0.0
36	1.5338846626105642E-4	0.0	0.0	31.202972054922274	0.0
37	2.3008269939158466E-4	0.0	0.0	31.6826177889206	0.0
38	2.3008269939158466E-4	0.0	0.0	32.11962152929835	0.0
39	2.3008269939158466E-4	0.0	0.0	32.65287653225491	0.0
40	2.3008269939158466E-4	0.0	0.0	33.15813814011883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAT	20	6.311767E-4	46.000004	14
ATCTACG	20	6.311767E-4	46.000004	28
TATTCGC	20	6.311767E-4	46.000004	23
CGTCGTT	20	6.311767E-4	46.000004	34
GACGCGT	20	6.311767E-4	46.000004	29
GAACCGA	20	6.311767E-4	46.000004	14
CCTTGCG	20	6.311767E-4	46.000004	22
GCCCGTT	35	1.0197073E-7	46.000004	44
CGAAGTT	20	6.311767E-4	46.000004	42
ACGCGTT	20	6.311767E-4	46.000004	30
TCGATGG	60	0.0	46.0	1
ACGATTA	25	3.4174096E-5	46.0	16
CGGTCTA	50	1.6370905E-11	46.0	30
CGATATC	25	3.4174096E-5	46.0	37
ATTACGG	135	0.0	44.296295	1
ATGACGG	125	0.0	44.16	1
CCATAGG	110	0.0	43.909092	1
CTAACGG	90	0.0	43.444443	1
CGCACTT	660	0.0	43.21212	34
CCGCACT	650	0.0	43.16923	33
>>END_MODULE