FastQCFastQC Report
Thu 26 May 2016
SRR1544979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544979_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences756191
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT39700.5249996363352645No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT31410.4153712487982533Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT26830.35480454012279966No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA21790.28815471223540084No Hit
TGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTTG19530.25826808306367044Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16500.21819884129803185No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10830.14321778492470816No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT10590.1400439835967368No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC9600.1269520531188549No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC9530.12602636106486323No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC8360.11055407959100282No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC8350.110421837869004No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG206.3100783E-446.030
TGATAGT206.3100783E-446.031
TCGTTCA206.3100783E-446.015
TAAACGG301.8605515E-646.01
TCCACGT206.3100783E-446.023
TTGCGTT206.3100783E-446.022
GTATCGG206.3100783E-446.01
CGAGCTA206.3100783E-446.024
CGTAAGG206.3100783E-446.01
CGGTCTA253.4160388E-546.030
ATAACGG206.3100783E-446.01
TATCGGG206.3100783E-446.02
AATACGG253.4160388E-546.01
CGCCCGT206.3100783E-446.046
TACGGGA1250.044.163
CGTTTTT14050.043.8718831
CGAATAT800.043.12514
ATCAACG4650.043.0322614
CAAACGG750.042.9333341
CAACGCA4450.042.8988816
Produced by FastQC (version 0.11.5)