##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544979_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	756191
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89873325654497	34.0	33.0	34.0	31.0	34.0
2	33.25348357756175	34.0	33.0	34.0	31.0	34.0
3	33.37693783713374	34.0	34.0	34.0	31.0	34.0
4	36.60224202615477	37.0	37.0	37.0	35.0	37.0
5	36.60461973231631	37.0	37.0	37.0	35.0	37.0
6	36.37862788634089	37.0	37.0	37.0	35.0	37.0
7	36.1592811868959	37.0	37.0	37.0	35.0	37.0
8	36.318882398759044	37.0	37.0	37.0	35.0	37.0
9	38.24174712473436	39.0	39.0	39.0	37.0	39.0
10	38.15698414818478	39.0	39.0	39.0	35.0	39.0
11	37.59811079475953	39.0	35.0	39.0	35.0	39.0
12	37.562635630416125	39.0	35.0	39.0	35.0	39.0
13	37.55425679491028	39.0	35.0	39.0	35.0	39.0
14	38.81619061850776	41.0	36.0	41.0	35.0	41.0
15	38.854187632489676	41.0	36.0	41.0	35.0	41.0
16	38.88385341798567	41.0	36.0	41.0	35.0	41.0
17	38.97883338997687	41.0	36.0	41.0	35.0	41.0
18	38.96491627115372	41.0	36.0	41.0	35.0	41.0
19	38.92130956332461	41.0	36.0	41.0	35.0	41.0
20	38.843879390259865	41.0	35.0	41.0	35.0	41.0
21	38.785380942116475	41.0	35.0	41.0	35.0	41.0
22	38.768662943621386	41.0	35.0	41.0	35.0	41.0
23	38.729026132286684	41.0	35.0	41.0	35.0	41.0
24	38.690295176747675	41.0	35.0	41.0	35.0	41.0
25	38.641348548184254	41.0	35.0	41.0	35.0	41.0
26	38.55912857994872	40.0	35.0	41.0	35.0	41.0
27	38.650180972796555	41.0	36.0	41.0	35.0	41.0
28	38.67209474854898	41.0	36.0	41.0	35.0	41.0
29	38.67962723703403	41.0	36.0	41.0	35.0	41.0
30	38.623719404224595	41.0	35.0	41.0	35.0	41.0
31	38.55750068435091	41.0	35.0	41.0	35.0	41.0
32	38.501813695217216	41.0	35.0	41.0	35.0	41.0
33	38.424104492119056	40.0	35.0	41.0	35.0	41.0
34	38.3255685402233	40.0	35.0	41.0	35.0	41.0
35	38.2569998849497	40.0	35.0	41.0	35.0	41.0
36	38.20835476751244	40.0	35.0	41.0	35.0	41.0
37	38.172615119725045	40.0	35.0	41.0	35.0	41.0
38	38.11623386155085	40.0	35.0	41.0	35.0	41.0
39	38.057990639930914	40.0	35.0	41.0	35.0	41.0
40	37.98915882363054	40.0	35.0	41.0	35.0	41.0
41	37.95915978899511	40.0	35.0	41.0	35.0	41.0
42	37.89487973276593	40.0	35.0	41.0	35.0	41.0
43	37.82345730113159	40.0	35.0	41.0	35.0	41.0
44	37.629301327310166	40.0	35.0	41.0	35.0	41.0
45	37.65610143469044	40.0	35.0	41.0	35.0	41.0
46	37.62161543842759	40.0	35.0	41.0	35.0	41.0
47	37.54526303539714	39.0	35.0	41.0	35.0	41.0
48	37.50217603753549	39.0	35.0	41.0	35.0	41.0
49	37.44010574048091	39.0	35.0	41.0	35.0	41.0
50	37.373125308288515	39.0	35.0	41.0	35.0	41.0
51	37.301056214633604	39.0	35.0	41.0	35.0	41.0
52	36.858900727461716	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	3.0
14	7.0
15	9.0
16	21.0
17	20.0
18	49.0
19	85.0
20	168.0
21	262.0
22	452.0
23	702.0
24	1237.0
25	2146.0
26	3045.0
27	3557.0
28	3458.0
29	3389.0
30	3857.0
31	4768.0
32	6407.0
33	9618.0
34	32409.0
35	138009.0
36	25663.0
37	43161.0
38	76869.0
39	393420.0
40	3397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.90889338804614	21.964821057113877	22.781016965290515	21.345268589549466
2	28.571485246452283	24.44276644392753	24.759882093280666	22.225866216339522
3	27.696574013708176	24.634649182547797	24.70434057004117	22.964436233702862
4	27.08839433423566	26.357097611582258	22.413120494689835	24.141387559492244
5	24.662155460723547	35.34781556511516	20.294740349990942	19.69528862417035
6	84.4577626552022	3.4505832521148756	8.440989115183863	3.6506649774990714
7	83.92654765793299	3.3552369705537357	6.686538189425688	6.031677182087594
8	70.92348361723427	6.303566162517142	13.684770117602563	9.088180102646024
9	48.05082313859858	19.92578594561427	16.105587080512727	15.917803835274421
10	38.92244155246492	20.441793144853616	26.713092327203046	13.922672975478417
11	38.04065375017687	17.018319445748496	25.446745597342467	19.49428120673216
12	21.928851308730202	30.08340485406465	28.813619839432103	19.174123997773048
13	14.97452363225693	33.371727513286984	30.31165406623459	21.342094788221495
14	14.27152663811127	23.863812185016748	41.767622201269255	20.097038975602725
15	15.14299958608341	18.65520748064973	45.23394221830199	20.967850714964868
16	16.518049011427006	21.587667665973278	29.35144692280125	32.54283639979846
17	19.09200188841179	23.65976320797259	32.63090938664967	24.617325516965952
18	23.319769740713657	27.478507414132142	27.89149831193442	21.31022453321978
19	27.02557951628623	22.274134444869087	25.566292114029395	25.13399392481529
20	21.00580408917853	30.189330473385695	26.933274794331062	21.871590643104717
21	19.897750700550525	24.06759667861691	25.661241670424534	30.37341095040803
22	18.754256530426836	29.69289504900217	23.82242052603112	27.730427894539872
23	18.21404909606171	22.186061458017882	36.079376771212566	23.520512674707845
24	19.709702971868218	22.84727006801192	32.101545773488446	25.34148118663142
25	17.849061943345003	28.657706849195506	28.877624832879523	24.61560637457997
26	18.28612083455106	28.35712141509222	27.159408138949022	26.1973496114077
27	20.796200959810417	26.278546028714967	33.183283059438686	19.74196995203593
28	17.87048510230881	26.723539423240954	28.655062014755533	26.750913459694708
29	19.17862021632101	29.660231343668464	25.99978047874148	25.161367961269043
30	24.17603489065593	25.98338250521363	21.621653788526974	28.218928815603466
31	25.452035266222424	30.69608075208512	21.024185688536363	22.827698293156093
32	21.702453480668243	28.120408732714353	21.507529182442003	28.6696086041754
33	17.31361521097183	30.348681748394256	24.404284102825873	27.933418937808042
34	17.955913254720038	28.18454596788377	25.671159799574443	28.18838097782174
35	23.687798453036336	26.527953916404716	23.02778001854029	26.756467612018657
36	18.77713434833263	31.981867015079523	25.409850156904803	23.831148479683044
37	18.960685858466974	32.01863021379519	24.157917774742096	24.86276615299574
38	18.34377822534254	28.30726628589867	25.027142613440255	28.321812875318535
39	23.523686476035817	23.72072664181404	27.814533629731113	24.94105325241903
40	15.628723430985028	22.150356193078206	31.810085018203072	30.410835357733696
41	19.711422114254205	27.11325577797144	24.232898831115417	28.94242327665894
42	20.556711201270577	22.185268007685888	28.02347555048923	29.234545240554304
43	21.91377575242234	27.733072728979845	27.15702778795304	23.19612373064477
44	17.30938347586787	29.83280679087691	26.64300421454368	26.214805518711543
45	20.038984859645247	28.404199468123792	25.310139898517704	26.246675773713257
46	21.75310206019379	23.979788175209702	27.63270126198275	26.63440850261376
47	20.037926925869257	20.60656633046413	31.729946534671793	27.625560208994816
48	19.40686942849095	20.393789399768046	28.753053130756644	31.446288040984356
49	19.01993014992244	24.523037169180803	30.295388334428736	26.161644346468023
50	18.358853781650403	25.18107197784687	28.477196898667135	27.982877341835593
51	18.634577772017916	21.69200638463034	30.598089636084	29.075326207267743
52	20.724393704765063	20.08315359479285	33.939308984105864	25.25314371633622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	263.5
2	442.0
3	1884.0
4	3326.0
5	2271.5
6	1217.0
7	1257.5
8	1298.0
9	1295.5
10	1293.0
11	1292.0
12	1291.0
13	1263.5
14	1165.5
15	1095.0
16	1021.0
17	947.0
18	936.5
19	926.0
20	1089.5
21	1253.0
22	1456.0
23	1659.0
24	1909.5
25	2160.0
26	2469.0
27	2778.0
28	4165.0
29	5552.0
30	5877.0
31	6202.0
32	6731.5
33	7261.0
34	8298.5
35	9336.0
36	10461.0
37	11586.0
38	13912.0
39	19285.5
40	22333.0
41	31093.5
42	39854.0
43	54507.0
44	69160.0
45	83989.0
46	98818.0
47	97765.0
48	96712.0
49	85292.5
50	73873.0
51	64340.0
52	54807.0
53	47527.0
54	40247.0
55	35717.5
56	31188.0
57	29888.0
58	28588.0
59	29606.5
60	30625.0
61	29174.0
62	27723.0
63	26685.5
64	19890.5
65	14133.0
66	11437.5
67	8742.0
68	7345.5
69	5949.0
70	5075.0
71	4201.0
72	3479.5
73	2758.0
74	2179.0
75	1600.0
76	1298.5
77	997.0
78	741.0
79	485.0
80	389.5
81	294.0
82	220.5
83	147.0
84	133.5
85	120.0
86	63.5
87	7.0
88	3.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	756191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.48339329636929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.85120344584705	18.49896893081073
2	12.042603640261456	6.378580170346654
3	4.451617616043858	3.5368182039220613
4	2.111367213073866	2.236646731875774
5	1.2572828195757275	1.6648557697796056
6	0.8470608531613885	1.3459827432138698
7	0.603778907105983	1.1193079982657836
8	0.5023685025930944	1.0643538107104829
9	0.4099818875709987	0.9771940415637553
>10	7.380715433015178	46.76294399130681
>50	0.4121555766884528	7.262245337788616
>100	0.11783036559767396	5.38128528639046
>500	0.008022492976862907	1.4180566458572168
>1k	0.004011246488431454	2.3527603381681965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	3970	0.5249996363352645	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	3141	0.4153712487982533	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	2683	0.35480454012279966	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2179	0.28815471223540084	No Hit
TGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTTG	1953	0.25826808306367044	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1650	0.21819884129803185	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1083	0.14321778492470816	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1059	0.1400439835967368	No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	960	0.1269520531188549	No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	953	0.12602636106486323	No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	836	0.11055407959100282	No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	835	0.110421837869004	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4211898845662009	0.0
2	0.0	0.0	0.0	1.5599233526979295	0.0
3	0.0	0.0	0.0	2.4220071384081536	0.0
4	1.3224172199880719E-4	0.0	0.0	2.988398433729045	0.0
5	1.3224172199880719E-4	0.0	0.0	5.984202403890023	0.0
6	1.3224172199880719E-4	0.0	0.0	6.550329215766916	0.0
7	1.3224172199880719E-4	0.0	0.0	9.21949613259084	0.0
8	1.3224172199880719E-4	0.0	0.0	12.07538836087708	0.0
9	1.3224172199880719E-4	0.0	0.0	14.726305920065169	0.0
10	1.3224172199880719E-4	0.0	0.0	15.987495222767793	0.0
11	1.3224172199880719E-4	0.0	0.0	17.195655594948896	0.0
12	1.3224172199880719E-4	0.0	0.0	18.24049744046147	0.0
13	1.3224172199880719E-4	0.0	0.0	18.643437967391836	0.0
14	1.3224172199880719E-4	0.0	0.0	18.965182077014934	0.0
15	1.3224172199880719E-4	0.0	0.0	20.001560452319588	0.0
16	1.3224172199880719E-4	0.0	0.0	21.022598787872376	0.0
17	1.3224172199880719E-4	0.0	0.0	22.397912696659972	0.0
18	1.3224172199880719E-4	0.0	0.0	23.009133935738458	0.0
19	1.3224172199880719E-4	0.0	0.0	23.801790817399308	0.0
20	1.3224172199880719E-4	0.0	0.0	24.712275073361095	0.0
21	1.3224172199880719E-4	0.0	0.0	25.52225562060379	0.0
22	1.3224172199880719E-4	0.0	0.0	26.39346408513193	0.0
23	1.3224172199880719E-4	0.0	0.0	27.013810003028336	0.0
24	1.3224172199880719E-4	0.0	0.0	27.536297046645622	0.0
25	1.3224172199880719E-4	0.0	0.0	28.040534732627073	0.0
26	1.3224172199880719E-4	0.0	0.0	28.52771323647068	0.0
27	1.3224172199880719E-4	0.0	0.0	28.99545220718046	0.0
28	1.3224172199880719E-4	0.0	0.0	29.45591788318031	0.0
29	1.3224172199880719E-4	0.0	0.0	30.037252493087063	0.0
30	1.3224172199880719E-4	0.0	0.0	30.609330182453903	0.0
31	1.3224172199880719E-4	0.0	0.0	31.123750481029262	0.0
32	1.3224172199880719E-4	0.0	0.0	31.599291713336974	0.0
33	1.3224172199880719E-4	0.0	0.0	32.05473220390087	0.0
34	1.3224172199880719E-4	0.0	0.0	32.69121161188112	0.0
35	1.3224172199880719E-4	0.0	0.0	33.16952992035081	0.0
36	1.3224172199880719E-4	0.0	0.0	33.6696681129503	0.0
37	1.3224172199880719E-4	0.0	0.0	34.12894361345216	0.0
38	1.3224172199880719E-4	0.0	0.0	34.564944570882226	0.0
39	1.3224172199880719E-4	0.0	0.0	35.08412557144954	0.0
40	1.3224172199880719E-4	0.0	0.0	35.58175117133105	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	6.3100783E-4	46.0	30
TGATAGT	20	6.3100783E-4	46.0	31
TCGTTCA	20	6.3100783E-4	46.0	15
TAAACGG	30	1.8605515E-6	46.0	1
TCCACGT	20	6.3100783E-4	46.0	23
TTGCGTT	20	6.3100783E-4	46.0	22
GTATCGG	20	6.3100783E-4	46.0	1
CGAGCTA	20	6.3100783E-4	46.0	24
CGTAAGG	20	6.3100783E-4	46.0	1
CGGTCTA	25	3.4160388E-5	46.0	30
ATAACGG	20	6.3100783E-4	46.0	1
TATCGGG	20	6.3100783E-4	46.0	2
AATACGG	25	3.4160388E-5	46.0	1
CGCCCGT	20	6.3100783E-4	46.0	46
TACGGGA	125	0.0	44.16	3
CGTTTTT	1405	0.0	43.871883	1
CGAATAT	80	0.0	43.125	14
ATCAACG	465	0.0	43.03226	14
CAAACGG	75	0.0	42.933334	1
CAACGCA	445	0.0	42.89888	16
>>END_MODULE