FastQCFastQC Report
Thu 26 May 2016
SRR1544978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544978_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences451068
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT22640.5019198879104703Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT17200.381317229331276Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT12560.27845025583725735Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.1908803107292027No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT6100.13523459877446414Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC5410.11993757038850018No Hit
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC5230.11594704124433568No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG4740.10508393412966559No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC4620.10242358136688925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG351.0177246E-746.0000041
CTCAAGG351.0177246E-746.0000041
AACGGCT351.0177246E-746.0000043
GACGGGT850.046.0000043
TTTGCGG1150.046.0000041
CGGGTTC301.8588707E-646.05
CGGAACC206.307357E-446.016
CTGTCGG253.4138313E-546.01
TCGTTCA206.307357E-446.015
GTACCGG253.4138313E-546.01
ACCGGGT301.8588707E-646.03
GTCGAGG206.307357E-446.01
CCGATTC301.8588707E-646.011
AACCCGC206.307357E-446.019
GCGAACA301.8588707E-646.033
CGTGACG206.307357E-446.018
ATTACGG253.4138313E-546.01
TGAACGT253.4138313E-546.043
TTCGTTC206.307357E-446.014
ATTCGTT206.307357E-446.013
Produced by FastQC (version 0.11.5)