##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544978_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	451068
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.935965308999975	34.0	33.0	34.0	31.0	34.0
2	33.240821782968425	34.0	33.0	34.0	31.0	34.0
3	33.28380199881171	34.0	34.0	34.0	31.0	34.0
4	36.57126863355414	37.0	37.0	37.0	35.0	37.0
5	36.564737467521525	37.0	37.0	37.0	35.0	37.0
6	36.67665407433026	37.0	37.0	37.0	35.0	37.0
7	36.52864756533383	37.0	37.0	37.0	35.0	37.0
8	36.55850115725345	37.0	37.0	37.0	35.0	37.0
9	38.34024581659528	39.0	39.0	39.0	37.0	39.0
10	38.150895208704675	39.0	39.0	39.0	35.0	39.0
11	37.34516081832451	39.0	35.0	39.0	35.0	39.0
12	37.23349694502824	39.0	35.0	39.0	35.0	39.0
13	37.20271001268102	39.0	35.0	39.0	35.0	39.0
14	38.475761969370474	40.0	35.0	41.0	35.0	41.0
15	38.55606693447551	41.0	35.0	41.0	35.0	41.0
16	38.561274575008646	41.0	35.0	41.0	35.0	41.0
17	38.649208101660946	41.0	36.0	41.0	35.0	41.0
18	38.630743036526646	40.0	36.0	41.0	35.0	41.0
19	38.52549061338867	40.0	35.0	41.0	35.0	41.0
20	38.43819557139943	40.0	35.0	41.0	35.0	41.0
21	38.38919409046973	40.0	35.0	41.0	35.0	41.0
22	38.36761197868171	40.0	35.0	41.0	35.0	41.0
23	38.37569945108055	40.0	35.0	41.0	35.0	41.0
24	38.31684358012539	40.0	35.0	41.0	35.0	41.0
25	38.28998288506389	40.0	35.0	41.0	35.0	41.0
26	38.234581038779076	40.0	35.0	41.0	35.0	41.0
27	38.24224285473587	40.0	35.0	41.0	35.0	41.0
28	38.268560394441636	40.0	35.0	41.0	35.0	41.0
29	38.25840893169101	40.0	36.0	41.0	35.0	41.0
30	38.162164019615666	40.0	35.0	41.0	35.0	41.0
31	38.02867416886146	40.0	35.0	41.0	35.0	41.0
32	37.94738709019482	40.0	35.0	41.0	35.0	41.0
33	37.885210212207475	40.0	35.0	41.0	35.0	41.0
34	37.652582315748404	40.0	35.0	41.0	35.0	41.0
35	37.680850780813536	40.0	35.0	41.0	35.0	41.0
36	37.47627187031667	39.0	35.0	41.0	34.0	41.0
37	37.3806410563374	39.0	35.0	41.0	34.0	41.0
38	37.26584461766297	39.0	35.0	41.0	34.0	41.0
39	37.279576471840166	39.0	35.0	41.0	34.0	41.0
40	37.27124956769268	39.0	35.0	41.0	34.0	41.0
41	37.28721611819061	39.0	35.0	41.0	34.0	41.0
42	37.2410257433469	39.0	35.0	41.0	34.0	41.0
43	37.198930094797234	39.0	35.0	41.0	34.0	41.0
44	37.09968341802123	39.0	35.0	41.0	34.0	41.0
45	37.039413126180534	38.0	35.0	41.0	34.0	41.0
46	37.02085494869953	39.0	35.0	41.0	34.0	41.0
47	37.02529108693146	38.0	35.0	41.0	34.0	41.0
48	37.04808809314782	38.0	35.0	41.0	34.0	41.0
49	36.968457084076014	38.0	35.0	41.0	34.0	41.0
50	36.90339372334105	38.0	35.0	41.0	34.0	41.0
51	36.82920535262976	38.0	35.0	41.0	34.0	41.0
52	36.64035134392154	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	2.0
15	4.0
16	7.0
17	21.0
18	31.0
19	61.0
20	103.0
21	178.0
22	302.0
23	455.0
24	920.0
25	1586.0
26	2179.0
27	2498.0
28	2470.0
29	2636.0
30	2981.0
31	3653.0
32	4878.0
33	7355.0
34	22509.0
35	105375.0
36	14454.0
37	21810.0
38	43964.0
39	209331.0
40	1300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.751168338254985	22.804100490391694	25.365355112754617	22.079376058598704
2	25.59835767556111	24.53443826651414	27.905992001206027	21.961212056718722
3	25.458245763388227	25.196644408381886	27.403185329041296	21.94192449918859
4	25.610772655120734	27.29987496342015	24.406741333900875	22.68261104755824
5	22.779713923399576	35.870201388704146	21.08484751744748	20.265237170448803
6	87.88076298917237	2.2207294687275536	7.286706217244407	2.611801324855676
7	87.70096748162139	1.7888655369035267	5.4282281163815655	5.081938865093512
8	78.10795711511346	3.7681679924091265	11.525091560474252	6.598783332003157
9	49.67499357081416	17.922131474633538	16.500172922929583	15.902702031622725
10	43.14848315553309	17.019163407734535	25.89099647946651	13.941356957265866
11	40.618044286005656	16.43831972119503	24.46216534979205	18.481470643007263
12	19.276916119077388	34.75107965982956	28.962817136218927	17.00918708487412
13	12.977200776823006	38.73052400081584	29.738975054758924	18.553300167602224
14	10.658259951936293	22.369797901868456	49.70647441184035	17.265467734354907
15	12.98872897212837	17.290297693474155	49.36417568969645	20.35679764470102
16	13.240354004274298	20.05750795888868	29.311101652079063	37.39103638475795
17	17.239307598854275	21.84105279026666	37.05738380909309	23.862255801785984
18	23.75118607394007	26.365426055494957	28.05585854017576	21.827529330389208
19	24.794931141202657	22.80454388251882	26.967995956263803	25.432529020014723
20	17.923461651014925	32.33060203783021	28.022825826704622	21.72311048445024
21	19.746911773834544	23.488032846488778	26.372963721656156	30.39209165802052
22	15.7257885728981	31.417879344134363	23.69598375411246	29.160348328855072
23	16.201991717435067	22.475103532061684	39.2488493974301	22.074055353073152
24	16.658907304441904	22.452490533578086	36.810857786409144	24.077744375570866
25	14.06949728200626	30.86275240096837	30.51202922840902	24.55572108861635
26	17.271896920198284	29.64453253168037	28.14365018134738	24.93992036677397
27	17.55965841070526	26.3381574396765	37.75550471325831	18.346679436359928
28	16.574662800287317	23.834987185967528	32.5272464462121	27.063103567533055
29	19.087365984729576	27.62133425558896	29.61881578830686	23.672483971374607
30	27.95343495880887	23.431722046343346	22.643814236434416	25.971028758413368
31	31.106396374825966	22.37711387196609	26.223540574813555	20.292949178394387
32	25.994750237214788	24.161102095471193	25.653338299325156	24.190809367988862
33	22.21283708886465	23.229091844245215	27.954986831253827	26.60308423563631
34	22.18069115964777	21.105465251358996	30.935025317690457	25.77881827130277
35	37.45399806681032	18.3273918788298	24.520693110573127	19.697916943786748
36	26.0982822988995	26.716814316245003	27.970948947830482	19.213954437025016
37	25.572419236124045	26.38205326026231	26.740535795046423	21.304991708567222
38	22.511461686486296	28.41500616315057	26.589117383631738	22.484414766731405
39	28.94973706846861	23.331737121675665	25.828699885604834	21.88982592425089
40	17.054191385777752	21.4351272978797	30.888025752214745	30.622655564127804
41	22.523654969982353	22.866618780316937	26.379614603563102	28.230111646137612
42	23.262567949843483	21.28636923922779	27.44065196378373	28.010410847145
43	25.142550568872103	26.04973086097883	27.698041093582344	21.109677476566727
44	18.11079482472709	30.459930653471318	27.1256218574583	24.303652664343293
45	24.93304778880346	27.298766483102327	26.26255908200094	21.505626646093273
46	24.865652185479796	21.5173765374622	29.108914842108064	24.50805643494994
47	24.62688552502062	19.517234651981518	30.759220339283655	25.096659483714205
48	22.063413942022045	18.43890499880284	29.75050325006429	29.74717780911082
49	22.46645738558266	24.14824372378444	29.99636418455754	23.38893470607536
50	21.148252591626985	25.965708052887816	27.047584843083527	25.838454512401675
51	20.24151569164738	20.6702758785815	32.038406626051945	27.049801803719177
52	23.748969113304426	18.704053490826215	34.05983133363484	23.48714606223452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	105.5
2	132.0
3	860.0
4	1588.0
5	1121.5
6	655.0
7	716.0
8	777.0
9	822.0
10	867.0
11	881.0
12	895.0
13	827.0
14	708.5
15	658.0
16	657.0
17	656.0
18	643.0
19	630.0
20	611.5
21	593.0
22	694.0
23	795.0
24	1034.0
25	1273.0
26	1484.0
27	1695.0
28	2126.5
29	2558.0
30	3139.5
31	3721.0
32	4193.5
33	4666.0
34	5287.5
35	5909.0
36	6716.5
37	7524.0
38	8357.5
39	10855.0
40	12519.0
41	17895.5
42	23272.0
43	30884.5
44	38497.0
45	41691.0
46	44885.0
47	44263.0
48	43641.0
49	48346.0
50	53051.0
51	54192.5
52	55334.0
53	48302.0
54	41270.0
55	34161.0
56	27052.0
57	22862.0
58	18672.0
59	16467.0
60	14262.0
61	12548.0
62	10834.0
63	9717.0
64	6715.5
65	4831.0
66	4126.0
67	3421.0
68	2585.5
69	1750.0
70	1568.0
71	1386.0
72	1178.0
73	970.0
74	716.0
75	462.0
76	422.5
77	383.0
78	317.0
79	251.0
80	146.0
81	41.0
82	25.0
83	9.0
84	28.0
85	47.0
86	26.5
87	6.0
88	3.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	451068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.559880067365803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0019306526282	18.85802762685229
2	11.595607882703874	6.159559093456328
3	3.8512652213305314	3.0686742715846775
4	1.8909859256437265	2.008974375766963
5	1.065708090011334	1.4152539528761257
6	0.7377385579129405	1.1756548571543461
7	0.555548378777735	1.0328708818369308
8	0.4653140546005752	0.9886948387080785
9	0.38010213217222377	0.9085920339459859
>10	7.892747483914187	50.80742127808475
>50	0.4736650871109067	8.262962082867007
>100	0.08353881606894298	3.5882125293124583
>500	0.0033415526427577192	0.5624609672153773
>1k	0.0025061644820682893	1.162641210338689
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	2264	0.5019198879104703	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT	1720	0.381317229331276	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1256	0.27845025583725735	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	861	0.1908803107292027	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	610	0.13523459877446414	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	541	0.11993757038850018	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	523	0.11594704124433568	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	474	0.10508393412966559	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	462	0.10242358136688925	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38907659155604035	0.0
2	0.0	0.0	0.0	1.432821658818626	0.0
3	0.0	0.0	0.0	2.233366144350741	0.0
4	0.0	0.0	0.0	2.7306304149263525	0.0
5	0.0	0.0	0.0	5.402067980880932	0.0
6	0.0	0.0	0.0	5.941897895660965	0.0
7	2.2169606356469535E-4	0.0	0.0	8.944992772708328	0.0
8	2.2169606356469535E-4	0.0	0.0	11.94808764975569	0.0
9	2.2169606356469535E-4	0.0	0.0	15.086417125577519	0.0
10	2.2169606356469535E-4	0.0	0.0	16.17006748428175	0.0
11	2.2169606356469535E-4	0.0	0.0	17.346386797556022	0.0
12	2.2169606356469535E-4	0.0	0.0	18.280835705481213	0.0
13	4.433921271293907E-4	0.0	0.0	18.587219665327623	0.0
14	4.433921271293907E-4	0.0	0.0	18.840396569918504	0.0
15	4.433921271293907E-4	0.0	0.0	19.7409259801183	0.0
16	4.433921271293907E-4	0.0	0.0	20.753633598481827	0.0
17	4.433921271293907E-4	0.0	0.0	22.371793166440536	0.0
18	4.433921271293907E-4	0.0	0.0	22.924481452907322	0.0
19	4.433921271293907E-4	0.0	0.0	23.70862042973565	0.0
20	4.433921271293907E-4	0.0	0.0	24.52645720822581	0.0
21	4.433921271293907E-4	0.0	0.0	25.200856633589613	0.0
22	4.433921271293907E-4	0.0	0.0	25.984552218290812	0.0
23	4.433921271293907E-4	0.0	0.0	26.51551429052826	0.0
24	4.433921271293907E-4	0.0	0.0	26.953585712132096	0.0
25	6.65088190694086E-4	0.0	0.0	27.421142710190036	0.0
26	6.65088190694086E-4	0.0	0.0	27.829506859276208	0.0
27	6.65088190694086E-4	0.0	0.0	28.255828389511116	0.0
28	6.65088190694086E-4	0.0	0.0	28.641136147986558	0.0
29	6.65088190694086E-4	0.0	0.0	29.24193248024688	0.0
30	6.65088190694086E-4	0.0	0.0	29.773781336738583	0.0
31	6.65088190694086E-4	0.0	0.0	30.194782161447943	0.0
32	6.65088190694086E-4	0.0	0.0	30.622655564127804	0.0
33	8.867842542587814E-4	0.0	0.0	31.025033919497726	0.0
34	8.867842542587814E-4	0.0	0.0	31.610533223372087	0.0
35	8.867842542587814E-4	0.0	0.0	32.04394902764106	0.0
36	8.867842542587814E-4	0.0	0.0	32.447214167265244	0.0
37	8.867842542587814E-4	0.0	0.0	32.83274362180425	0.0
38	8.867842542587814E-4	0.0	0.0	33.209183537737104	0.0
39	8.867842542587814E-4	0.0	0.0	33.65457092943858	0.0
40	8.867842542587814E-4	0.0	0.0	34.11924587867018	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	35	1.0177246E-7	46.000004	1
CTCAAGG	35	1.0177246E-7	46.000004	1
AACGGCT	35	1.0177246E-7	46.000004	3
GACGGGT	85	0.0	46.000004	3
TTTGCGG	115	0.0	46.000004	1
CGGGTTC	30	1.8588707E-6	46.0	5
CGGAACC	20	6.307357E-4	46.0	16
CTGTCGG	25	3.4138313E-5	46.0	1
TCGTTCA	20	6.307357E-4	46.0	15
GTACCGG	25	3.4138313E-5	46.0	1
ACCGGGT	30	1.8588707E-6	46.0	3
GTCGAGG	20	6.307357E-4	46.0	1
CCGATTC	30	1.8588707E-6	46.0	11
AACCCGC	20	6.307357E-4	46.0	19
GCGAACA	30	1.8588707E-6	46.0	33
CGTGACG	20	6.307357E-4	46.0	18
ATTACGG	25	3.4138313E-5	46.0	1
TGAACGT	25	3.4138313E-5	46.0	43
TTCGTTC	20	6.307357E-4	46.0	14
ATTCGTT	20	6.307357E-4	46.0	13
>>END_MODULE