FastQCFastQC Report
Thu 26 May 2016
SRR1544977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544977_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences269947
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT14970.5545533012035696Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT11800.43712284263207224Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT8830.32710124580010147Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTTG7040.26079193323133804Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.2011505962281485No Hit
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC4420.16373584444353892No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT4310.15966097048679928Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA4150.15373388109517794No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG3610.133729954398456No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC3520.13039596661566902No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC3330.12335754796311868No Hit
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC3260.12076444635428435No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT2990.11076248300592338Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCCC750.046.0000047
TAGTAGG750.046.0000041
GTGTTGC206.302833E-446.033
CGTGGGA551.8189894E-1246.03
ACTATGA206.302833E-446.025
AGATCGC206.302833E-446.019
GCGACCG206.302833E-446.045
GGATGTC206.302833E-446.07
CGACGGT206.302833E-446.027
GGCACGA253.410162E-546.07
TTTCGTG253.410162E-546.015
GACCGGG206.302833E-446.01
CGACCGG206.302833E-446.046
GCACGAC206.302833E-446.08
ATATGTC206.302833E-446.045
CACGAGT206.302833E-446.038
CACGACG206.302833E-446.025
TACGGGT206.302833E-446.03
TACGGGA501.6370905E-1146.03
ACGGGTG453.074092E-1046.04
Produced by FastQC (version 0.11.5)