##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544977_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	269947
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94215531196865	34.0	33.0	34.0	31.0	34.0
2	33.285467147254835	34.0	34.0	34.0	31.0	34.0
3	33.39964141109181	34.0	34.0	34.0	31.0	34.0
4	36.62406694647468	37.0	37.0	37.0	35.0	37.0
5	36.62650816641785	37.0	37.0	37.0	35.0	37.0
6	36.452311009198105	37.0	37.0	37.0	35.0	37.0
7	36.2993883984634	37.0	37.0	37.0	35.0	37.0
8	36.42813218891116	37.0	37.0	37.0	35.0	37.0
9	38.28610430936443	39.0	39.0	39.0	37.0	39.0
10	38.17025564277432	39.0	39.0	39.0	35.0	39.0
11	37.44073095829922	39.0	35.0	39.0	35.0	39.0
12	37.38562754911149	39.0	35.0	39.0	35.0	39.0
13	37.372280484687735	39.0	35.0	39.0	35.0	39.0
14	38.59622814848841	41.0	35.0	41.0	35.0	41.0
15	38.64839023956554	41.0	35.0	41.0	35.0	41.0
16	38.684867770340105	41.0	35.0	41.0	35.0	41.0
17	38.78678036799816	41.0	36.0	41.0	35.0	41.0
18	38.7835167644019	41.0	36.0	41.0	35.0	41.0
19	38.70704990238825	41.0	35.0	41.0	35.0	41.0
20	38.63156841898595	41.0	35.0	41.0	35.0	41.0
21	38.58489999888867	41.0	35.0	41.0	35.0	41.0
22	38.5654406235298	40.0	35.0	41.0	35.0	41.0
23	38.54894479286675	40.0	35.0	41.0	35.0	41.0
24	38.508740604637204	40.0	35.0	41.0	35.0	41.0
25	38.47532293376107	40.0	35.0	41.0	35.0	41.0
26	38.40593523913954	40.0	35.0	41.0	35.0	41.0
27	38.51006308645771	40.0	35.0	41.0	35.0	41.0
28	38.53112277595232	40.0	36.0	41.0	35.0	41.0
29	38.50162809736726	40.0	36.0	41.0	35.0	41.0
30	38.428002533830714	40.0	35.0	41.0	35.0	41.0
31	38.293887318621806	40.0	35.0	41.0	35.0	41.0
32	38.244359077893066	40.0	35.0	41.0	35.0	41.0
33	38.20296206292346	40.0	35.0	41.0	35.0	41.0
34	37.958143635602546	40.0	35.0	41.0	35.0	41.0
35	37.97212045327416	40.0	35.0	41.0	35.0	41.0
36	37.81001085398245	40.0	35.0	41.0	35.0	41.0
37	37.722289930986456	40.0	35.0	41.0	35.0	41.0
38	37.651253764627874	40.0	35.0	41.0	35.0	41.0
39	37.694673398852366	40.0	35.0	41.0	35.0	41.0
40	37.718166899428404	40.0	35.0	41.0	35.0	41.0
41	37.718870741293664	40.0	35.0	41.0	35.0	41.0
42	37.68621988760757	40.0	35.0	41.0	35.0	41.0
43	37.64282247996829	40.0	35.0	41.0	35.0	41.0
44	37.48696225555387	39.0	35.0	41.0	35.0	41.0
45	37.48061286104309	39.0	35.0	41.0	35.0	41.0
46	37.39436259710239	39.0	35.0	41.0	35.0	41.0
47	37.36045223692058	39.0	35.0	41.0	35.0	41.0
48	37.33626230334102	39.0	35.0	41.0	35.0	41.0
49	37.265689190841165	39.0	35.0	41.0	35.0	41.0
50	37.193675054732964	39.0	35.0	41.0	35.0	41.0
51	37.12159053443824	39.0	35.0	41.0	34.0	41.0
52	36.66569363615821	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	2.0
16	4.0
17	6.0
18	19.0
19	38.0
20	59.0
21	102.0
22	157.0
23	240.0
24	473.0
25	814.0
26	1169.0
27	1315.0
28	1265.0
29	1218.0
30	1357.0
31	1703.0
32	2227.0
33	3513.0
34	12203.0
35	60359.0
36	7752.0
37	12243.0
38	25740.0
39	135511.0
40	457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.715833107980455	22.434403790373665	24.85006316054633	22.99969994109955
2	25.629475415544533	24.523332357833205	27.705068031872926	22.14212419474934
3	25.58724490362923	25.260514100916104	27.140142324234017	22.01209867122065
4	25.75579650820346	27.023082308749498	24.290694099212068	22.930427083834974
5	22.80077200339326	35.75331453952072	20.879654154334	20.566259302752023
6	86.49512682119082	2.3041560009927875	8.016017959080857	3.1846992187355294
7	86.2606363471348	1.7896105531826616	6.018218391017497	5.931534708665034
8	76.02973917102246	3.808154934116697	12.843632268556421	7.3184736263044226
9	48.72660188851886	17.397489136756477	17.140031191307923	16.735877783416743
10	42.017136697203526	16.503609967882586	27.43575590764113	14.043497427272762
11	39.39180653980226	16.35765539161391	24.956380326508537	19.294157742075296
12	18.77738963574331	33.35321377900106	30.165551015569726	17.703845569685903
13	13.03848533230597	37.2280484687735	30.329657303100237	19.40380889582029
14	10.68209685604952	22.19583844236091	48.89144906222332	18.230615639366246
15	12.824739671120627	17.25449810518361	49.098897190930074	20.82186503276569
16	13.055525714306881	20.72592027323882	29.819557172333827	36.39899684012047
17	16.707724108806545	21.880961818431025	37.30695284629946	24.104361226462974
18	23.121205273627787	27.017155219357875	28.091810614676216	21.769828892338126
19	24.16918876668383	22.995995510229786	26.864532667523626	25.97028305556276
20	17.541221054503293	33.13761590238084	27.477615976469455	21.843547066646416
21	19.250075014725113	23.755033395444293	26.181806058226243	30.81308553160435
22	15.649738652402137	32.14038311224055	23.435341011383716	28.774537223973596
23	15.867559187544222	22.987104876142354	38.716488792244405	22.42884714406902
24	16.731802909460004	22.87449017770155	36.086713317799415	24.306993595039025
25	14.191674662063294	30.683060008075657	30.563406890982304	24.56185843887874
26	17.90610749517498	28.277773044338332	27.91140483131874	25.904714629167948
27	18.52215434881662	25.628364086283607	37.2765765131674	18.572905051732377
28	17.66680126098827	23.592038437174704	31.941455174534262	26.799705127302765
29	19.908352380282054	26.801927785824624	29.148314298732714	24.141405535160608
30	28.092551500850167	22.79743801561047	21.94319625704305	27.16681422649631
31	31.40023782446184	21.872441627430568	25.744312772507193	20.983007775600395
32	25.87248608060101	23.298277069202474	25.780986638117852	25.048250212078667
33	22.56554064316329	22.159905462924204	28.324819316384325	26.949734577528183
34	23.368661255727975	20.23100830903844	31.005345493745068	25.394984941488513
35	38.00153363438008	17.78386127647279	24.343667460649684	19.870937628497444
36	27.00641236983556	26.04511255913198	27.411306663900692	19.53716840713177
37	26.644119030772707	25.545014391713927	27.03604781679367	20.774818760719697
38	22.80484687735	28.088847069980403	26.343319244147928	22.762986808521674
39	29.41725597987753	22.982659559098636	25.85507525551312	21.745009205510712
40	17.30895323896913	21.080063864388197	30.760482613253714	30.85050028338896
41	22.80966263748069	23.005256587404194	26.013254453652014	28.1718263214631
42	23.262714532852748	21.09265892934539	27.447610086424373	28.197016451377493
43	25.848777723034523	25.692080297243532	27.377596342985843	21.0815456367361
44	18.509559283859424	29.01532523050821	28.062545610805085	24.41256987482728
45	24.91266804224533	26.700055936906132	26.459638373458493	21.927637647390043
46	25.179016621781315	20.623307538146378	29.26981963126095	24.92785620881136
47	24.49740134174486	18.715896083305243	31.022756318832954	25.76394625611694
48	21.913190366997966	17.84831837360667	29.349835338047843	30.888655921347524
49	22.102116341355895	23.085272294191082	30.38522376614669	24.427387598306336
50	21.324556301792576	24.90155474963604	27.860654128402984	25.9132348201684
51	20.332139271782978	19.705349568619024	32.151125961762865	27.811385197835133
52	23.614265022393287	18.279884569934097	34.387490877838985	23.718359529833634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	58.5
2	86.0
3	535.0
4	984.0
5	690.0
6	396.0
7	442.5
8	489.0
9	522.0
10	555.0
11	547.0
12	539.0
13	505.5
14	431.5
15	391.0
16	393.0
17	395.0
18	375.0
19	355.0
20	368.5
21	382.0
22	431.0
23	480.0
24	601.0
25	722.0
26	808.5
27	895.0
28	1103.5
29	1312.0
30	1617.0
31	1922.0
32	2215.0
33	2508.0
34	2846.5
35	3185.0
36	3631.0
37	4077.0
38	4514.0
39	5937.0
40	6923.0
41	9911.5
42	12900.0
43	17097.5
44	21295.0
45	23292.5
46	25290.0
47	25538.0
48	25786.0
49	29743.0
50	33700.0
51	34465.5
52	35231.0
53	30801.0
54	26371.0
55	21813.5
56	17256.0
57	14334.0
58	11412.0
59	9870.0
60	8328.0
61	7349.5
62	6371.0
63	5975.5
64	4301.5
65	3023.0
66	2547.0
67	2071.0
68	1585.5
69	1100.0
70	960.0
71	820.0
72	711.5
73	603.0
74	439.5
75	276.0
76	264.0
77	252.0
78	204.0
79	156.0
80	87.0
81	18.0
82	16.0
83	14.0
84	28.0
85	42.0
86	21.5
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	269947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.02406398292999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03983897834536	23.07008412762505
2	9.958587821580602	6.37828907155849
3	3.29909309642791	3.1695110521695
4	1.7154821395521005	2.1974683919436035
5	1.2041921154913937	1.92815626771181
6	0.8895521006848048	1.7092244033087975
7	0.7715620951323339	1.7295987730924958
8	0.7530538589672404	1.929267596972739
9	0.7333888580418286	2.1137482542869526
>10	8.421247455117527	48.09499642522421
>50	0.1700444197667962	3.5143935661444656
>100	0.038173237090505276	2.3845421508666518
>500	0.0034702942809550254	0.789043775259588
>1k	0.0023135295206366834	0.9916761438356418
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1497	0.5545533012035696	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT	1180	0.43712284263207224	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	883	0.32710124580010147	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTTG	704	0.26079193323133804	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	543	0.2011505962281485	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	442	0.16373584444353892	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	431	0.15966097048679928	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	415	0.15373388109517794	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	361	0.133729954398456	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	352	0.13039596661566902	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	333	0.12335754796311868	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	326	0.12076444635428435	No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	299	0.11076248300592338	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4445317043715989	0.0
2	0.0	0.0	0.0	1.619577176260525	0.0
3	0.0	0.0	0.0	2.586433633268753	0.0
4	0.0	0.0	0.0	3.125428324819316	0.0
5	0.0	0.0	0.0	6.141946382067591	0.0
6	0.0	0.0	0.0	6.7798493778408355	0.0
7	0.0	0.0	0.0	10.232008505373276	0.0
8	0.0	0.0	0.0	13.61822876342393	0.0
9	0.0	0.0	0.0	17.26487051161895	0.0
10	0.0	0.0	0.0	18.517338588685927	0.0
11	0.0	0.0	0.0	19.776104198231504	0.0
12	0.0	0.0	0.0	20.84038718711451	0.0
13	0.0	0.0	0.0	21.188233245785284	0.0
14	0.0	0.0	0.0	21.464213345582653	0.0
15	0.0	0.0	0.0	22.455889489418293	0.0
16	0.0	0.0	0.0	23.483869055777614	0.0
17	0.0	0.0	0.0	25.10529844747302	0.0
18	0.0	0.0	0.0	25.690598524895627	0.0
19	0.0	0.0	0.0	26.44889552393618	0.0
20	0.0	0.0	0.0	27.274613164806425	0.0
21	0.0	0.0	0.0	27.993643196627485	0.0
22	0.0	0.0	0.0	28.790466276713577	0.0
23	0.0	0.0	0.0	29.345019577917146	0.0
24	0.0	0.0	0.0	29.82029805850778	0.0
25	0.0	0.0	0.0	30.291501665141677	0.0
26	0.0	0.0	0.0	30.733810710991417	0.0
27	0.0	0.0	0.0	31.16982222436256	0.0
28	0.0	0.0	0.0	31.531004234164485	0.0
29	0.0	0.0	0.0	32.09630038489037	0.0
30	0.0	0.0	0.0	32.639740393484644	0.0
31	0.0	0.0	0.0	33.08204943933439	0.0
32	0.0	0.0	0.0	33.513615635661814	0.0
33	0.0	0.0	0.0	33.9051739785958	0.0
34	0.0	0.0	0.0	34.471951901669584	0.0
35	0.0	0.0	0.0	34.907222528866775	0.0
36	0.0	0.0	0.0	35.3232301155412	0.0
37	0.0	0.0	0.0	35.72367909256261	0.0
38	0.0	0.0	0.0	36.116719207844504	0.0
39	0.0	0.0	0.0	36.554582936650526	0.0
40	0.0	0.0	0.0	37.003189514978864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCCC	75	0.0	46.000004	7
TAGTAGG	75	0.0	46.000004	1
GTGTTGC	20	6.302833E-4	46.0	33
CGTGGGA	55	1.8189894E-12	46.0	3
ACTATGA	20	6.302833E-4	46.0	25
AGATCGC	20	6.302833E-4	46.0	19
GCGACCG	20	6.302833E-4	46.0	45
GGATGTC	20	6.302833E-4	46.0	7
CGACGGT	20	6.302833E-4	46.0	27
GGCACGA	25	3.410162E-5	46.0	7
TTTCGTG	25	3.410162E-5	46.0	15
GACCGGG	20	6.302833E-4	46.0	1
CGACCGG	20	6.302833E-4	46.0	46
GCACGAC	20	6.302833E-4	46.0	8
ATATGTC	20	6.302833E-4	46.0	45
CACGAGT	20	6.302833E-4	46.0	38
CACGACG	20	6.302833E-4	46.0	25
TACGGGT	20	6.302833E-4	46.0	3
TACGGGA	50	1.6370905E-11	46.0	3
ACGGGTG	45	3.074092E-10	46.0	4
>>END_MODULE