FastQCFastQC Report
Thu 26 May 2016
SRR1544976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544976_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences526186
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT21900.41620263556993153No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT16540.3143375156313547Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT15470.29400250101675074No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.19061700615371752No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT6950.13208257156214723No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA6150.11687882231758352No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT5640.10718643217417416No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG5460.1037655885941473No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC5390.102435260535248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTG351.0181611E-746.0000045
CGAATAT351.0181611E-746.00000414
CTACCGG351.0181611E-746.0000041
CACACGG351.0181611E-746.0000041
CGGGTCA253.414612E-546.05
CGGGTAC453.092282E-1046.05
GTACCGG206.3083187E-446.01
ACACGTA206.3083187E-446.028
CGTATTC206.3083187E-446.031
GTCGAAT206.3083187E-446.029
CATGCGG600.046.01
GACACCG206.3083187E-446.08
CGTTGAT800.046.024
CTCCCGA206.3083187E-446.038
GAATCCG301.8594656E-646.029
ATTCGCA206.3083187E-446.010
ATTCGAA206.3083187E-446.041
AGGGTAC453.092282E-1046.05
TGACGTA253.414612E-546.013
TGCGGGC850.046.03
Produced by FastQC (version 0.11.5)