##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544976_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	526186
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94572641613422	34.0	33.0	34.0	31.0	34.0
2	33.23756618382093	34.0	33.0	34.0	31.0	34.0
3	33.282683309704176	34.0	34.0	34.0	31.0	34.0
4	36.56954384951329	37.0	37.0	37.0	35.0	37.0
5	36.55887081754361	37.0	37.0	37.0	35.0	37.0
6	36.66948949610974	37.0	37.0	37.0	35.0	37.0
7	36.51257350062525	37.0	37.0	37.0	35.0	37.0
8	36.54966494737602	37.0	37.0	37.0	35.0	37.0
9	38.34160163896417	39.0	39.0	39.0	37.0	39.0
10	38.12080519055999	39.0	39.0	39.0	35.0	39.0
11	37.2281740677251	39.0	35.0	39.0	35.0	39.0
12	37.10905839379991	39.0	35.0	39.0	35.0	39.0
13	37.08931062399988	39.0	35.0	39.0	35.0	39.0
14	38.32031068861581	40.0	35.0	41.0	35.0	41.0
15	38.39364977403428	40.0	35.0	41.0	35.0	41.0
16	38.42239436244978	40.0	35.0	41.0	35.0	41.0
17	38.514082472737776	40.0	36.0	41.0	35.0	41.0
18	38.49594249942036	40.0	36.0	41.0	35.0	41.0
19	38.37860186321947	40.0	35.0	41.0	35.0	41.0
20	38.28409915885257	40.0	35.0	41.0	35.0	41.0
21	38.23564861094746	40.0	35.0	41.0	35.0	41.0
22	38.208441501674315	40.0	35.0	41.0	35.0	41.0
23	38.20880449118753	40.0	35.0	41.0	35.0	41.0
24	38.152145819159	40.0	35.0	41.0	35.0	41.0
25	38.118220553188415	40.0	35.0	41.0	35.0	41.0
26	38.07511412314277	40.0	35.0	41.0	35.0	41.0
27	38.07932556168351	40.0	35.0	41.0	35.0	41.0
28	38.102955988946874	40.0	35.0	41.0	35.0	41.0
29	38.11623456344334	40.0	35.0	41.0	35.0	41.0
30	37.9953248471073	40.0	35.0	41.0	35.0	41.0
31	37.9289851877473	40.0	35.0	41.0	35.0	41.0
32	37.82513597853231	40.0	35.0	41.0	35.0	41.0
33	37.764233559995894	40.0	35.0	41.0	35.0	41.0
34	37.67476519709761	40.0	35.0	41.0	35.0	41.0
35	37.61301897047812	40.0	35.0	41.0	35.0	41.0
36	37.55785406681288	40.0	35.0	41.0	35.0	41.0
37	37.513436313394884	40.0	35.0	41.0	35.0	41.0
38	37.4715290790709	40.0	35.0	41.0	35.0	41.0
39	37.37060469111683	39.0	35.0	41.0	34.0	41.0
40	37.29026047823393	39.0	35.0	41.0	34.0	41.0
41	37.278285625235185	39.0	35.0	41.0	34.0	41.0
42	37.150095213479645	39.0	35.0	41.0	34.0	41.0
43	37.06529250113078	38.0	35.0	41.0	34.0	41.0
44	36.973969280824655	38.0	35.0	41.0	34.0	41.0
45	36.95015830903901	38.0	35.0	41.0	34.0	41.0
46	36.94394567700395	38.0	35.0	41.0	34.0	41.0
47	36.880954643415066	38.0	35.0	41.0	34.0	41.0
48	36.892002067709896	38.0	35.0	41.0	34.0	41.0
49	36.73901624140513	37.0	35.0	41.0	34.0	41.0
50	36.51318925246966	37.0	35.0	40.0	34.0	41.0
51	36.410130638215385	37.0	35.0	40.0	34.0	41.0
52	36.15865682477299	36.0	35.0	39.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	1.0
14	7.0
15	15.0
16	11.0
17	33.0
18	63.0
19	89.0
20	169.0
21	244.0
22	382.0
23	624.0
24	1077.0
25	1823.0
26	2555.0
27	3001.0
28	2942.0
29	3146.0
30	3693.0
31	4473.0
32	6101.0
33	8811.0
34	27666.0
35	131640.0
36	17326.0
37	24296.0
38	48382.0
39	235922.0
40	1690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.83265993393971	22.87556111337056	24.797124971017855	20.49465398167188
2	26.20157130748443	25.179879358249746	26.76886120117221	21.84968813309362
3	26.447301904649688	25.001805445222793	26.06986882965339	22.48102382047413
4	26.164512168700803	27.4294641058485	22.80277316386221	23.60325056158849
5	22.655866936786612	36.62526178955731	21.422082685590265	19.296788588065816
6	88.18668683697399	2.6251173539394816	6.702762901331469	2.4854329077550523
7	87.761932092454	2.3187618066615228	5.142098041377004	4.777208059507474
8	78.1413796642252	4.352453314987477	11.032600639317655	6.47356638146967
9	52.55137917010335	19.7561318621172	13.6162877765657	14.076201191213752
10	45.72698627481537	19.193403093202786	24.946311760480132	10.133298871501713
11	44.69180099812613	16.33167739164478	22.401394183805724	16.575127426423357
12	19.676502225448797	37.81077413690216	25.9393066330157	16.57341700463334
13	13.409706833705194	40.3214832777763	27.35572592201237	18.913083966506143
14	12.144754896557492	22.65738731171107	49.13908769902658	16.05877009270486
15	13.007377619320925	16.95256050141965	50.91165481407715	19.128407065182273
16	13.252728122755073	20.10904889145663	28.931974624942512	37.706248360845784
17	18.252481061829847	22.234533035846642	36.085908785106405	23.427077117217106
18	25.211237091066657	27.280467363251777	26.661104628401368	20.8471909172802
19	28.822697677247213	22.731125495547204	25.283454899978334	23.162721927227253
20	18.617941184296047	32.87145610107453	26.650081910199052	21.860520804430372
21	19.42982139395575	24.943651104362335	24.459791784654094	31.166735717027823
22	16.66615987502518	33.082408121842846	22.674301482745644	27.577130520386326
23	17.324292170449233	22.406145355444654	39.6148890316352	20.654673442470912
24	16.275993660036566	22.681333216771254	36.89455819805164	24.14811492514054
25	14.954217710087308	33.23254514563291	29.366801853337034	22.446435290942745
26	17.810622099409716	31.84045185542755	26.71203718837065	23.636888856792083
27	18.5424925786699	27.830082898442754	37.69940667368573	15.928017849201614
28	16.083476185227276	25.72455367493624	32.3300125811025	25.861957558733984
29	20.637759271436337	29.699003774330752	26.5326329472848	23.130604006948115
30	28.28486504772077	26.571402507858437	21.25123055345448	23.89250189096631
31	32.158590308370044	26.8790883831953	21.15088580844036	19.8114354999943
32	26.486641605819994	29.581934905147612	21.173121291710537	22.758302197321857
33	20.965590114522243	30.31646604052559	23.95559745033125	24.762346394620913
34	19.928314322311884	28.084745698289197	27.1489549322863	24.837985047112618
35	29.793076972781492	26.433428483464024	22.298388782673808	21.47510576108068
36	24.21748203106886	31.006146115632117	24.066014679219897	20.710357174079128
37	22.520553568509992	32.05235411052366	24.18859490750419	21.23849741346216
38	20.73715378212267	32.785935011573855	23.923859623783226	22.55305158252025
39	26.888400679607592	26.502225448795674	24.077607538018874	22.53176633357786
40	16.447415932769022	23.559729829375925	29.154329457644256	30.8385247802108
41	22.50782042851767	26.182756667794276	22.834130896679124	28.475292007008928
42	21.60832861383617	25.27642316595272	26.254214289243727	26.861033930967377
43	23.026268277757296	29.378394712136014	26.854192243807322	20.741144766299367
44	17.132534883102174	33.51647516277514	24.779260565655488	24.571729388467194
45	23.48979258285093	30.54623270098406	24.26803449730704	21.69594021885797
46	23.794627755204434	25.056728989368775	26.29317389668292	24.855469358743868
47	22.54716012968798	21.36335820413314	31.181939466272386	24.907542199906494
48	20.854032604440256	21.290000114028118	28.43519211837639	29.420775163155234
49	20.976992926455665	27.446378276883078	28.847023676038514	22.729605120622747
50	19.210507311102916	29.10643764752388	25.836111185018222	25.846943856354976
51	19.940287274841975	22.243845332258935	31.624558616154747	26.191308776744343
52	23.880528938436218	20.01155484942587	31.925592851197106	24.182323360940806
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	132.5
2	182.0
3	979.0
4	1776.0
5	1226.5
6	677.0
7	796.5
8	916.0
9	975.0
10	1034.0
11	946.0
12	858.0
13	839.5
14	765.0
15	709.0
16	645.0
17	581.0
18	669.0
19	757.0
20	668.5
21	580.0
22	710.0
23	840.0
24	1140.5
25	1441.0
26	1777.5
27	2114.0
28	3075.0
29	4036.0
30	4119.0
31	4202.0
32	5446.0
33	6690.0
34	7237.0
35	7784.0
36	8320.5
37	8857.0
38	10710.0
39	14296.0
40	16029.0
41	23535.0
42	31041.0
43	41415.0
44	51789.0
45	57363.0
46	62937.0
47	63780.0
48	64623.0
49	63512.0
50	62401.0
51	54877.5
52	47354.0
53	40894.5
54	34435.0
55	28604.5
56	22774.0
57	20155.0
58	17536.0
59	16540.0
60	15544.0
61	15638.0
62	15732.0
63	13424.5
64	8454.0
65	5791.0
66	4710.0
67	3629.0
68	2861.0
69	2093.0
70	1710.5
71	1328.0
72	1120.0
73	912.0
74	830.5
75	749.0
76	656.5
77	564.0
78	389.5
79	215.0
80	136.0
81	57.0
82	42.0
83	27.0
84	15.0
85	3.0
86	3.0
87	3.0
88	2.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	526186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.981353097880167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.72757383679462	17.668704957733574
2	11.585860055595884	5.788609219829326
3	4.242909820262121	3.1798088514729392
4	1.8603332994908164	1.8589457213729812
5	1.0980845324451962	1.3715818718167052
6	0.7527608733080469	1.1282991104626172
7	0.5481780213001283	0.9585960097417046
8	0.4445318368574924	0.8884005423829022
9	0.37143109110636646	0.8350966114613134
>10	7.539495059535646	48.37844857025922
>50	0.6964095863846101	11.26353348439244
>100	0.1255820565611592	4.901652485274091
>500	0.003805516865489673	0.5626062724564017
>1k	0.0030444134923917386	1.2157162913437758
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	2190	0.41620263556993153	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT	1654	0.3143375156313547	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	1547	0.29400250101675074	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1003	0.19061700615371752	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	695	0.13208257156214723	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	615	0.11687882231758352	No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	564	0.10718643217417416	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	546	0.1037655885941473	No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	539	0.102435260535248	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3261204212958916	0.0
2	0.0	0.0	0.0	1.262671374761016	0.0
3	1.9004686555704637E-4	0.0	0.0	1.9409486379341145	0.0
4	1.9004686555704637E-4	0.0	0.0	2.381857366026462	0.0
5	1.9004686555704637E-4	0.0	0.0	4.8990281003295415	0.0
6	1.9004686555704637E-4	0.0	0.0	5.401702059727929	0.0
7	1.9004686555704637E-4	0.0	0.0	8.149399641951705	0.0
8	1.9004686555704637E-4	0.0	0.0	10.888355068359857	0.0
9	1.9004686555704637E-4	0.0	0.0	13.713971865462023	0.0
10	1.9004686555704637E-4	0.0	0.0	14.729392268133322	0.0
11	1.9004686555704637E-4	0.0	0.0	15.767618294671466	0.0
12	1.9004686555704637E-4	0.0	0.0	16.629860923703784	0.0
13	1.9004686555704637E-4	0.0	0.0	16.929184736956135	0.0
14	1.9004686555704637E-4	0.0	0.0	17.157621069355702	0.0
15	1.9004686555704637E-4	0.0	0.0	18.031836650918116	0.0
16	1.9004686555704637E-4	0.0	0.0	18.919355513069522	0.0
17	1.9004686555704637E-4	0.0	0.0	20.319240724762725	0.0
18	1.9004686555704637E-4	0.0	0.0	20.828376277590053	0.0
19	1.9004686555704637E-4	0.0	0.0	21.547513616857916	0.0
20	1.9004686555704637E-4	0.0	0.0	22.31929393788508	0.0
21	1.9004686555704637E-4	0.0	0.0	22.932005032441	0.0
22	1.9004686555704637E-4	0.0	0.0	23.680219542139092	0.0
23	1.9004686555704637E-4	0.0	0.0	24.163888814981775	0.0
24	1.9004686555704637E-4	0.0	0.0	24.55652563922263	0.0
25	1.9004686555704637E-4	0.0	0.0	24.978999821355945	0.0
26	1.9004686555704637E-4	0.0	0.0	25.34217938143546	0.0
27	1.9004686555704637E-4	0.0	0.0	25.74013751791192	0.0
28	1.9004686555704637E-4	0.0	0.0	26.110348812017044	0.0
29	1.9004686555704637E-4	0.0	0.0	26.684100299133767	0.0
30	1.9004686555704637E-4	0.0	0.0	27.202358101507834	0.0
31	1.9004686555704637E-4	0.0	0.0	27.622171627523347	0.0
32	1.9004686555704637E-4	0.0	0.0	28.04027473174885	0.0
33	1.9004686555704637E-4	0.0	0.0	28.431011087334138	0.0
34	1.9004686555704637E-4	0.0	0.0	29.001151684005276	0.0
35	1.9004686555704637E-4	0.0	0.0	29.42419600673526	0.0
36	1.9004686555704637E-4	0.0	0.0	29.85294173543196	0.0
37	1.9004686555704637E-4	0.0	0.0	30.2801670892042	0.0
38	1.9004686555704637E-4	0.0	0.0	30.65569969554492	0.0
39	1.9004686555704637E-4	0.0	0.0	31.095088048712814	0.0
40	1.9004686555704637E-4	0.0	0.0	31.53656691740183	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTG	35	1.0181611E-7	46.000004	5
CGAATAT	35	1.0181611E-7	46.000004	14
CTACCGG	35	1.0181611E-7	46.000004	1
CACACGG	35	1.0181611E-7	46.000004	1
CGGGTCA	25	3.414612E-5	46.0	5
CGGGTAC	45	3.092282E-10	46.0	5
GTACCGG	20	6.3083187E-4	46.0	1
ACACGTA	20	6.3083187E-4	46.0	28
CGTATTC	20	6.3083187E-4	46.0	31
GTCGAAT	20	6.3083187E-4	46.0	29
CATGCGG	60	0.0	46.0	1
GACACCG	20	6.3083187E-4	46.0	8
CGTTGAT	80	0.0	46.0	24
CTCCCGA	20	6.3083187E-4	46.0	38
GAATCCG	30	1.8594656E-6	46.0	29
ATTCGCA	20	6.3083187E-4	46.0	10
ATTCGAA	20	6.3083187E-4	46.0	41
AGGGTAC	45	3.092282E-10	46.0	5
TGACGTA	25	3.414612E-5	46.0	13
TGCGGGC	85	0.0	46.0	3
>>END_MODULE