FastQCFastQC Report
Thu 26 May 2016
SRR1544975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544975_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences329677
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT18020.5465956072155473No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT14040.4258713832023466Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT10980.3330532612223479No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTTG7860.2384151760662709Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.19746600460450683No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG6080.18442293517594494No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT4920.14923698043842912No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC4370.13255398465771043No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC3660.11101775374078265No Hit
GCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC3560.10798448178065197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC351.0165968E-746.0000048
CAGTAGG700.046.0000041
GTCGGCG351.0165968E-746.0000043
GAAATAG206.304874E-446.033
TTGGCGG253.4118173E-546.01
CCCCTGA206.304874E-446.022
AAATCCG206.304874E-446.023
CCGGGAT253.4118173E-546.04
GGATGCG206.304874E-446.07
GTGGGCG206.304874E-446.04
TAGAAGT206.304874E-446.040
TTCACGG301.8573373E-646.01
GCCGGGA206.304874E-446.03
AGGGTTA253.4118173E-546.05
AGTGGGA501.6370905E-1146.03
TGAAAGG453.092282E-1046.01
TCGATGG253.4118173E-546.01
TATGCCA405.5897544E-946.09
CAGGGTT501.6370905E-1146.04
ATCCGAA206.304874E-446.025
Produced by FastQC (version 0.11.5)