##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544975_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	329677
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.945816662976185	34.0	33.0	34.0	31.0	34.0
2	33.271062282173155	34.0	34.0	34.0	31.0	34.0
3	33.387091001192076	34.0	34.0	34.0	31.0	34.0
4	36.621023001301275	37.0	37.0	37.0	35.0	37.0
5	36.613740115325	37.0	37.0	37.0	35.0	37.0
6	36.44355232545795	37.0	37.0	37.0	35.0	37.0
7	36.277565617255675	37.0	37.0	37.0	35.0	37.0
8	36.407332024982026	37.0	37.0	37.0	35.0	37.0
9	38.28771797850624	39.0	39.0	39.0	37.0	39.0
10	38.12812844086788	39.0	39.0	39.0	35.0	39.0
11	37.32463896480495	39.0	35.0	39.0	35.0	39.0
12	37.2656114924608	39.0	35.0	39.0	35.0	39.0
13	37.264276852798346	39.0	35.0	39.0	35.0	39.0
14	38.43993363200951	40.0	35.0	41.0	35.0	41.0
15	38.48535991288443	41.0	35.0	41.0	35.0	41.0
16	38.53891839588446	41.0	35.0	41.0	35.0	41.0
17	38.6583534793146	41.0	36.0	41.0	35.0	41.0
18	38.646902877665106	40.0	36.0	41.0	35.0	41.0
19	38.56525326304231	40.0	35.0	41.0	35.0	41.0
20	38.47210451441866	40.0	35.0	41.0	35.0	41.0
21	38.43351219526991	40.0	35.0	41.0	35.0	41.0
22	38.39829894108476	40.0	35.0	41.0	35.0	41.0
23	38.36982258392305	40.0	35.0	41.0	35.0	41.0
24	38.340126851433375	40.0	35.0	41.0	35.0	41.0
25	38.29940517536862	40.0	35.0	41.0	35.0	41.0
26	38.24784865186228	40.0	35.0	41.0	35.0	41.0
27	38.335079486891715	40.0	35.0	41.0	35.0	41.0
28	38.34565347294473	40.0	35.0	41.0	35.0	41.0
29	38.37651094859514	40.0	36.0	41.0	35.0	41.0
30	38.23741722959139	40.0	35.0	41.0	35.0	41.0
31	38.19033478222624	40.0	35.0	41.0	35.0	41.0
32	38.09622448639122	40.0	35.0	41.0	35.0	41.0
33	38.050042920798234	40.0	35.0	41.0	35.0	41.0
34	37.955753661917576	40.0	35.0	41.0	35.0	41.0
35	37.91263266773235	40.0	35.0	41.0	35.0	41.0
36	37.88954340157184	40.0	35.0	41.0	35.0	41.0
37	37.82843207139109	40.0	35.0	41.0	35.0	41.0
38	37.803710904916024	40.0	35.0	41.0	35.0	41.0
39	37.73551385143641	40.0	35.0	41.0	35.0	41.0
40	37.676983835693726	40.0	35.0	41.0	35.0	41.0
41	37.66104399154324	40.0	35.0	41.0	35.0	41.0
42	37.5660297806641	40.0	35.0	41.0	35.0	41.0
43	37.49993781792482	39.0	35.0	41.0	35.0	41.0
44	37.36016767927396	39.0	35.0	41.0	35.0	41.0
45	37.36287639113435	39.0	35.0	41.0	35.0	41.0
46	37.28838529833747	39.0	35.0	41.0	35.0	41.0
47	37.19565514124431	39.0	35.0	41.0	35.0	41.0
48	37.14534529251358	38.0	35.0	41.0	35.0	41.0
49	36.99312660573835	38.0	35.0	41.0	35.0	41.0
50	36.72928957737422	37.0	35.0	40.0	34.0	41.0
51	36.61222954588885	37.0	35.0	40.0	34.0	41.0
52	36.11602568574696	36.0	35.0	39.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	4.0
15	4.0
16	10.0
17	15.0
18	23.0
19	49.0
20	91.0
21	122.0
22	192.0
23	318.0
24	558.0
25	1042.0
26	1425.0
27	1643.0
28	1554.0
29	1577.0
30	1828.0
31	2187.0
32	2980.0
33	4441.0
34	15722.0
35	78951.0
36	10230.0
37	14908.0
38	31838.0
39	157483.0
40	480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.01732908270823	22.311838557133193	24.42481580456022	22.24601655559836
2	25.79494474895125	25.388182979097724	26.781668117581752	22.035204154369275
3	26.50230377005372	25.078789239164394	26.119504848685228	22.299402142096657
4	26.053682847150395	27.03767627101678	23.073493146322004	23.835147735510816
5	22.790792199637828	36.03041765121619	21.55382389429654	19.624966254849443
6	86.70850559790341	2.6246902271010715	7.5219078067320435	3.144896368263482
7	86.16130333629583	2.1554430548688566	5.931259990839519	5.751993617995796
8	75.77174021845624	4.403097577325686	12.539849610376216	7.2853125938418515
9	51.13823530303904	19.144495976364745	14.48630022719207	15.230968493404152
10	44.20660221974842	18.857851776132396	26.57297900672476	10.362566997394419
11	42.95386090021445	16.0933277116693	23.192700734355142	17.760110653761103
12	19.19060171015873	35.920309879063446	27.47962399560782	17.40946441517
13	13.386435814448689	38.66966758372589	28.085975060437946	19.85792154138748
14	12.20194311401766	22.121652405233	48.55601088337979	17.120393597369546
15	12.92780509407693	16.87621520457903	50.40479014307944	19.791189558264605
16	13.108588102900717	20.66992844511446	29.585018063134523	36.6364653888503
17	17.57174446503699	22.399803443976985	36.46387221431887	23.564579876667164
18	24.457878468925646	27.926121628139057	27.016443367295867	20.59955653563943
19	27.933704808039383	22.99553805694665	25.27534526218086	23.795411872833107
20	18.116823436272472	33.5306982288725	26.322734070013983	22.029744264841042
21	18.9461199901722	25.257752284812103	24.40388622803532	31.39224149698038
22	16.402721451602627	33.575893981078444	22.64610512713959	27.37527944017933
23	16.768534049994386	22.974608480421747	39.04033341725386	21.21652405233001
24	16.666616112133998	22.8969567182424	35.85388122313658	24.582545946487016
25	15.057465337284675	32.66136248509905	29.710595522283935	22.570576655332342
26	18.440170227222403	30.276907397240326	26.530209872086925	24.752712503450347
27	19.153292465049123	27.470827506923445	36.84272788213919	16.53315214588825
28	16.74305456552929	25.774925154014383	31.640969797711094	25.84105048274523
29	21.613579351911113	28.80061393424473	25.786754914658893	23.799051799185264
30	28.057158976816705	26.49350728136934	20.507648395247468	24.941685346566487
31	32.91373071218192	26.557812646924113	20.138499197699566	20.389957443194398
32	26.26661853875157	29.32264003858322	20.83402845815753	23.576712964507685
33	21.26930298443628	30.308453425625686	23.728073235318206	24.694170354619825
34	20.268929891985184	28.29739411605905	26.60209841754202	24.831577574413743
35	29.39270862086224	26.933331715588288	21.902346842515552	21.77161282103392
36	25.051793118719228	30.5432286753398	22.816271684102926	21.58870652183804
37	23.291888727451415	31.955520099976642	23.86214385595598	20.89044731661596
38	21.117942713625762	33.1105900623944	22.95216226791678	22.819304956063053
39	26.846276810332537	26.831413777727896	23.861840528759966	22.460468883179598
40	16.717878408260205	23.49208467682004	28.535505964929307	31.254530949990446
41	22.62426556902665	26.383096182020584	22.4419659242228	28.550672324729966
42	21.39488044358569	25.023280362294003	26.48956402782117	27.092275166299135
43	23.82240799327827	28.696572706012248	26.562665882060323	20.918353418649165
44	17.414317650306206	31.76806389284057	25.976031084971048	24.841587371882177
45	23.376820342335073	29.888951913539618	24.451205270613357	22.283022473511952
46	24.130588424427547	23.724129981770037	26.67338030860509	25.47190128519733
47	22.329734861697965	20.37206113862963	31.358268851026917	25.939935148645493
48	20.655975394097858	20.494301998622895	28.215192445939515	30.634530161339736
49	20.868304431307006	26.05064957519026	29.259851309008518	23.821194684494216
50	19.54670783827807	27.780524574052784	26.576315605880907	26.09645198178824
51	19.70777457936101	21.34725807381164	31.799003266833903	27.145964079993444
52	23.8114882142218	19.23003424564043	32.65347597800271	24.305001562135057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	90.0
2	146.0
3	654.0
4	1162.0
5	794.5
6	427.0
7	507.5
8	588.0
9	610.0
10	632.0
11	607.5
12	583.0
13	552.0
14	501.0
15	481.0
16	437.5
17	394.0
18	442.0
19	490.0
20	423.0
21	356.0
22	421.0
23	486.0
24	677.5
25	869.0
26	1005.0
27	1141.0
28	1596.5
29	2052.0
30	2168.5
31	2285.0
32	2945.0
33	3605.0
34	3908.5
35	4212.0
36	4532.5
37	4853.0
38	6194.0
39	8288.0
40	9041.0
41	13448.5
42	17856.0
43	24078.0
44	30300.0
45	34200.5
46	38101.0
47	40409.5
48	42718.0
49	42182.5
50	41647.0
51	36988.5
52	32330.0
53	27575.0
54	22820.0
55	18944.0
56	15068.0
57	13018.0
58	10968.0
59	10366.5
60	9765.0
61	9654.0
62	9543.0
63	8333.0
64	5338.0
65	3553.0
66	2878.5
67	2204.0
68	1768.5
69	1333.0
70	1090.0
71	847.0
72	703.5
73	560.0
74	526.5
75	493.0
76	419.0
77	345.0
78	248.0
79	151.0
80	96.5
81	42.0
82	27.5
83	13.0
84	7.5
85	2.0
86	1.0
87	0.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	329677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.9954197593402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.56378933743225	21.46585900745275
2	10.358182994903325	6.213960937523697
3	3.6253134859639187	3.262283993120539
4	1.754510152900251	2.1050907403306875
5	1.1012458538953158	1.6516165822911515
6	0.8595582881643881	1.5469686996666434
7	0.7604562737642585	1.5967143598127862
8	0.6512418089151363	1.562741713859323
9	0.5824771458619853	1.572448184131741
>10	8.43985114472939	49.2827828450271
>50	0.23460885041663293	4.589037148481696
>100	0.06269719278375535	3.2246714208149188
>500	0.0030337351346978398	0.6203041158467226
>1k	0.0030337351346978398	1.3055202516402418
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	1802	0.5465956072155473	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT	1404	0.4258713832023466	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	1098	0.3330532612223479	No Hit
TGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTTG	786	0.2384151760662709	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	651	0.19746600460450683	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	608	0.18442293517594494	No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	492	0.14923698043842912	No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	437	0.13255398465771043	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	366	0.11101775374078265	No Hit
GCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	356	0.10798448178065197	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4337578902986863	0.0
2	0.0	0.0	0.0	1.5860979079523292	0.0
3	0.0	0.0	0.0	2.4396606375331005	0.0
4	0.0	0.0	0.0	2.9814030096124386	0.0
5	0.0	0.0	0.0	5.887580874613637	0.0
6	0.0	0.0	0.0	6.462992565450426	0.0
7	0.0	0.0	0.0	9.764405766856651	0.0
8	0.0	0.0	0.0	12.969967574322746	0.0
9	0.0	0.0	0.0	16.368445478453154	0.0
10	0.0	0.0	0.0	17.589944096797776	0.0
11	0.0	0.0	0.0	18.76139372780024	0.0
12	0.0	0.0	0.0	19.803019318909115	0.0
13	3.033271960130673E-4	0.0	0.0	20.162158718988586	0.0
14	3.033271960130673E-4	0.0	0.0	20.437276485772436	0.0
15	3.033271960130673E-4	0.0	0.0	21.429763071127194	0.0
16	3.033271960130673E-4	0.0	0.0	22.39919678958496	0.0
17	3.033271960130673E-4	0.0	0.0	23.843034242607157	0.0
18	3.033271960130673E-4	0.0	0.0	24.411166080739633	0.0
19	3.033271960130673E-4	0.0	0.0	25.152801074991583	0.0
20	3.033271960130673E-4	0.0	0.0	25.985130900851438	0.0
21	3.033271960130673E-4	0.0	0.0	26.64881080572803	0.0
22	6.066543920261346E-4	0.0	0.0	27.455964474318804	0.0
23	6.066543920261346E-4	0.0	0.0	28.028949547587487	0.0
24	6.066543920261346E-4	0.0	0.0	28.47211058096258	0.0
25	6.066543920261346E-4	0.0	0.0	28.915878268729696	0.0
26	6.066543920261346E-4	0.0	0.0	29.318393457839036	0.0
27	6.066543920261346E-4	0.0	0.0	29.749421403373606	0.0
28	6.066543920261346E-4	0.0	0.0	30.148903320522816	0.0
29	6.066543920261346E-4	0.0	0.0	30.701565471658622	0.0
30	6.066543920261346E-4	0.0	0.0	31.244521152522015	0.0
31	6.066543920261346E-4	0.0	0.0	31.688288840289133	0.0
32	9.09981588039202E-4	0.0	0.0	32.111733605923376	0.0
33	9.09981588039202E-4	0.0	0.0	32.49574583607592	0.0
34	9.09981588039202E-4	0.0	0.0	33.06327101981636	0.0
35	9.09981588039202E-4	0.0	0.0	33.489445730214726	0.0
36	9.09981588039202E-4	0.0	0.0	33.94352654264629	0.0
37	9.09981588039202E-4	0.0	0.0	34.375161142572885	0.0
38	9.09981588039202E-4	0.0	0.0	34.75826339113738	0.0
39	9.09981588039202E-4	0.0	0.0	35.204154369276594	0.0
40	9.09981588039202E-4	0.0	0.0	35.640945531535415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAC	35	1.0165968E-7	46.000004	8
CAGTAGG	70	0.0	46.000004	1
GTCGGCG	35	1.0165968E-7	46.000004	3
GAAATAG	20	6.304874E-4	46.0	33
TTGGCGG	25	3.4118173E-5	46.0	1
CCCCTGA	20	6.304874E-4	46.0	22
AAATCCG	20	6.304874E-4	46.0	23
CCGGGAT	25	3.4118173E-5	46.0	4
GGATGCG	20	6.304874E-4	46.0	7
GTGGGCG	20	6.304874E-4	46.0	4
TAGAAGT	20	6.304874E-4	46.0	40
TTCACGG	30	1.8573373E-6	46.0	1
GCCGGGA	20	6.304874E-4	46.0	3
AGGGTTA	25	3.4118173E-5	46.0	5
AGTGGGA	50	1.6370905E-11	46.0	3
TGAAAGG	45	3.092282E-10	46.0	1
TCGATGG	25	3.4118173E-5	46.0	1
TATGCCA	40	5.5897544E-9	46.0	9
CAGGGTT	50	1.6370905E-11	46.0	4
ATCCGAA	20	6.304874E-4	46.0	25
>>END_MODULE