FastQCFastQC Report
Thu 26 May 2016
SRR1544974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544974_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1342290
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT52070.38791915308912384TruSeq Adapter, Index 27 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT50470.37599922520468754TruSeq Adapter, Index 27 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT39730.2959867092804088TruSeq Adapter, Index 27 (100% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.15816254311661415No Hit
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC18770.13983565399429335TruSeq Adapter, Index 27 (100% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17810.13268369726363155No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT17090.12731972971563524TruSeq Adapter, Index 27 (96% over 26bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC15200.11323931490214484TruSeq Adapter, Index 27 (96% over 25bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGAAGG14840.11055733112814668No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG14650.10914183969186987No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14320.10668335456570488No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT13790.10273487845398536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA405.6134013E-946.07
GATCGTA253.4174634E-546.08
TTAATCG2000.046.019
CTATCGG253.4174634E-546.01
ATATGCG206.3118356E-446.018
TACGCGG1050.046.01
CGCCGAA206.3118356E-446.042
TATCGGT206.3118356E-446.038
AATACGG501.6370905E-1146.01
ATACCGG600.046.01
TCGAACA253.4174634E-546.033
TAGTAGG2650.045.1320721
TAATAGG1450.044.413791
CCCGCAC6950.044.34532532
CATGCGG2100.043.8095251
ACGTAGG1050.043.8095251
ATCAACG8150.043.74233214
CGCACTT7000.043.3714334
CGAATAT2400.043.12514
GGCGATA3150.043.0793657
Produced by FastQC (version 0.11.5)