##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544974_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1342290
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.889325704579484	34.0	33.0	34.0	31.0	34.0
2	33.199246809556804	34.0	33.0	34.0	31.0	34.0
3	33.26678586594551	34.0	34.0	34.0	31.0	34.0
4	36.55174142696437	37.0	37.0	37.0	35.0	37.0
5	36.54454030053118	37.0	37.0	37.0	35.0	37.0
6	36.63427277264972	37.0	37.0	37.0	35.0	37.0
7	36.44854241631838	37.0	37.0	37.0	35.0	37.0
8	36.48007658553666	37.0	37.0	37.0	35.0	37.0
9	38.28873492315371	39.0	39.0	39.0	37.0	39.0
10	38.11063332066841	39.0	39.0	39.0	35.0	39.0
11	37.46764112077122	39.0	35.0	39.0	35.0	39.0
12	37.37876986344233	39.0	35.0	39.0	35.0	39.0
13	37.36218700876859	39.0	35.0	39.0	35.0	39.0
14	38.66651990255459	41.0	35.0	41.0	35.0	41.0
15	38.73644443451117	41.0	35.0	41.0	35.0	41.0
16	38.736950286450764	41.0	35.0	41.0	35.0	41.0
17	38.7972047769111	41.0	36.0	41.0	35.0	41.0
18	38.79234889628918	41.0	36.0	41.0	35.0	41.0
19	38.72051047091165	41.0	36.0	41.0	35.0	41.0
20	38.6313047106065	41.0	35.0	41.0	35.0	41.0
21	38.56954905422822	41.0	35.0	41.0	35.0	41.0
22	38.547754211087025	40.0	35.0	41.0	35.0	41.0
23	38.53205343107674	40.0	35.0	41.0	35.0	41.0
24	38.47343718570502	40.0	35.0	41.0	35.0	41.0
25	38.41868374196336	40.0	35.0	41.0	35.0	41.0
26	38.38239650150117	40.0	35.0	41.0	35.0	41.0
27	38.34615172578206	40.0	35.0	41.0	35.0	41.0
28	38.39018244939618	40.0	36.0	41.0	35.0	41.0
29	38.386873924412754	40.0	36.0	41.0	35.0	41.0
30	38.32668722854227	40.0	35.0	41.0	35.0	41.0
31	38.28893979691423	40.0	35.0	41.0	35.0	41.0
32	38.19829098033957	40.0	35.0	41.0	35.0	41.0
33	38.138436552458856	40.0	35.0	41.0	35.0	41.0
34	38.04602805653026	40.0	35.0	41.0	35.0	41.0
35	37.9761318344024	40.0	35.0	41.0	35.0	41.0
36	37.91475538072994	40.0	35.0	41.0	35.0	41.0
37	37.876639921328476	40.0	35.0	41.0	35.0	41.0
38	37.82449917677998	40.0	35.0	41.0	35.0	41.0
39	37.71455125196493	40.0	35.0	41.0	35.0	41.0
40	37.649285176824684	40.0	35.0	41.0	34.0	41.0
41	37.61727570048201	40.0	35.0	41.0	35.0	41.0
42	37.52040617154266	40.0	35.0	41.0	35.0	41.0
43	37.434491801324604	39.0	35.0	41.0	34.0	41.0
44	37.35670086195979	39.0	35.0	41.0	34.0	41.0
45	37.31563596540241	39.0	35.0	41.0	34.0	41.0
46	37.329798329720106	39.0	35.0	41.0	34.0	41.0
47	37.28469108761892	39.0	35.0	41.0	34.0	41.0
48	37.311299346638954	39.0	35.0	41.0	34.0	41.0
49	37.225045258476186	39.0	35.0	41.0	34.0	41.0
50	37.14565928376133	39.0	35.0	41.0	34.0	41.0
51	37.08045802322896	38.0	35.0	41.0	34.0	41.0
52	36.8865394214365	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	5.0
14	11.0
15	18.0
16	20.0
17	61.0
18	102.0
19	215.0
20	355.0
21	560.0
22	893.0
23	1472.0
24	2377.0
25	3735.0
26	5448.0
27	6500.0
28	6983.0
29	7766.0
30	9267.0
31	11777.0
32	15056.0
33	21576.0
34	65660.0
35	258679.0
36	51241.0
37	78310.0
38	131391.0
39	656467.0
40	6339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.78339256047501	22.330271401858017	23.559066967644846	19.327269070022126
2	27.989331664543432	23.584694812596386	25.868403996155827	22.557569526704363
3	28.052879780077333	24.371261053870626	24.400390377638214	23.17546878841383
4	26.334175178240173	26.181674600868664	22.147747506127587	25.33640271476358
5	23.715665020226627	36.11455050696944	20.568059063242668	19.601725409561272
6	86.2672000834395	3.247435353016114	7.457702880897571	3.0276616826468197
7	85.96659440210387	3.230449455780793	5.802173896847924	5.000782245267416
8	73.9241147591057	6.238443257418293	12.04739661325049	7.79004537022551
9	49.45056582407676	20.19794530243092	15.077665780122029	15.273823093370284
10	40.301797674124074	20.274083841792756	25.070215825194257	14.353902658888915
11	38.624887319431714	17.418143620231096	24.202519574756572	19.754449485580615
12	21.070186025374547	31.237363013953768	28.395279708557762	19.297171252113927
13	15.007785202899523	33.42481877984638	30.673997422315598	20.8933985949385
14	14.495228303868762	24.343100224243646	41.993086441827025	19.168585030060566
15	16.577192707984118	17.65512668648355	45.16043477936959	20.60724582616275
16	16.686111049028156	20.972964113567112	28.601717959606344	33.73920687779839
17	19.261783966207005	23.633790015570405	32.20935863338027	24.89506738484232
18	23.90571337043411	27.795036840027116	27.30296731704773	20.99628247249104
19	27.28791840809363	22.6979266775436	25.172727205000413	24.841427709362357
20	20.824039514560937	30.103703372594598	27.15910868739244	21.913148425452025
21	20.54146272415052	23.797614524432127	25.617340515089882	30.04358223632747
22	19.57095709570957	28.5480037845771	23.53172563305992	28.349313486653404
23	19.09348948438862	22.066170499668477	36.5678802643244	22.272459751618502
24	19.835355996096222	21.61909870445284	32.72087253872114	25.8246727607298
25	16.84390109439838	28.90075915040714	29.443413867346102	24.81192588784838
26	18.114043910034344	28.49905758070164	27.009066595147104	26.377831914116918
27	19.70103330874848	27.835341096186365	32.700608661317595	19.76301693374755
28	17.930104522867637	25.39004239024354	28.563201692629757	28.116651394259062
29	19.205089809206655	26.46738037234875	26.727458298877295	27.600071519567305
30	24.777060098786404	26.219967369197416	23.48523791431062	25.51773461770556
31	25.81014534862064	23.214655551333916	24.539779034336842	26.435420065708605
32	22.598246280610002	25.77408756677022	23.907724858264608	27.71994129435517
33	16.88450334875474	24.919577736554693	28.219460772262327	29.976458142428235
34	17.623687876688347	23.451713117135643	29.193095381772938	29.73150362440307
35	24.683786663090686	21.481870534683264	27.470963800669008	26.363379001557043
36	19.6919443637366	25.807910362142312	28.035446885546346	26.464698388574746
37	18.56610717505159	26.567358767479455	31.29718615202378	23.569347905445174
38	18.899269159421586	24.18262819509942	29.106228907315113	27.811873738163882
39	23.4458276527427	20.127692227462024	29.77091388597099	26.655566233824285
40	15.591265672842681	20.31103561823451	32.60852721841033	31.48917149051248
41	19.81479412049557	23.496710844899386	25.87190547497188	30.81658955963316
42	20.27855381474942	21.110639280632352	28.974066706896423	29.636740197721807
43	21.417204925910198	25.363669549799223	27.47960574838522	25.739519775905357
44	17.439301492225972	27.454126902532238	27.7385661816746	27.368005423567187
45	20.462567701465407	25.205209008485497	28.418598067481692	25.913625222567404
46	21.768693799402513	21.483435025218096	29.097810458246727	27.650060717132664
47	20.713929180728456	19.090956499713176	33.34488076347138	26.850233556086984
48	19.78402580664387	18.52587741844162	29.071661116450247	32.618435658464264
49	19.14683116167147	23.16272936548734	32.23714696526086	25.45329250758033
50	19.052663731384424	23.566889420319008	29.363252352323265	28.017194495973296
51	19.190264398900386	20.1605465286935	31.655901481796032	28.993287590610077
52	21.27200530436791	18.180423008440798	34.574868322046655	25.97270336514464
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	156.0
1	394.0
2	632.0
3	2531.5
4	4431.0
5	3055.0
6	1679.0
7	1756.5
8	1834.0
9	1818.5
10	1803.0
11	1736.0
12	1669.0
13	1601.0
14	1559.5
15	1586.0
16	1437.0
17	1288.0
18	1424.5
19	1561.0
20	1940.0
21	2319.0
22	2457.0
23	2595.0
24	2977.5
25	3360.0
26	4278.0
27	5196.0
28	7411.5
29	9627.0
30	10141.0
31	10655.0
32	12010.0
33	13365.0
34	16161.0
35	18957.0
36	21448.5
37	23940.0
38	25481.0
39	32705.5
40	38389.0
41	50484.0
42	62579.0
43	82370.5
44	102162.0
45	118299.0
46	134436.0
47	145419.0
48	156402.0
49	159472.0
50	162542.0
51	141829.5
52	121117.0
53	106236.0
54	91355.0
55	79310.5
56	67266.0
57	60934.0
58	54602.0
59	52448.0
60	50294.0
61	50823.0
62	51352.0
63	45194.5
64	32303.5
65	25570.0
66	21116.0
67	16662.0
68	13535.5
69	10409.0
70	9194.0
71	7979.0
72	6810.5
73	5642.0
74	4958.0
75	4274.0
76	3385.0
77	2496.0
78	1885.0
79	1274.0
80	979.0
81	684.0
82	519.0
83	354.0
84	271.5
85	189.0
86	99.5
87	10.0
88	6.5
89	2.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1342290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.703032466068404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.55975752009996	13.986918533383383
2	12.711951419328804	5.263518858828971
3	5.413576593007051	3.3623235588771894
4	2.8242438728152592	2.338816503639563
5	1.6869881287644466	1.746288499984116
6	1.0429643732390466	1.2955515168072398
7	0.7161018329025525	1.0377835646914844
8	0.51408658101337	0.8514520941671924
9	0.4242875021074615	0.7905634137970059
>10	4.672838420435163	24.897650965568374
>50	2.090351505551917	29.293988771243434
>100	0.3255109356292684	10.841664692640805
>500	0.009393212348899506	1.2662857327398473
>1k	0.007225547960691928	2.260241497794769
>5k	7.225547960691928E-4	0.7669517958366446
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	5207	0.38791915308912384	TruSeq Adapter, Index 27 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	5047	0.37599922520468754	TruSeq Adapter, Index 27 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT	3973	0.2959867092804088	TruSeq Adapter, Index 27 (100% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2123	0.15816254311661415	No Hit
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1877	0.13983565399429335	TruSeq Adapter, Index 27 (100% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1781	0.13268369726363155	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	1709	0.12731972971563524	TruSeq Adapter, Index 27 (96% over 26bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1520	0.11323931490214484	TruSeq Adapter, Index 27 (96% over 25bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGAAGG	1484	0.11055733112814668	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	1465	0.10914183969186987	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1432	0.10668335456570488	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1379	0.10273487845398536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30433065879951426	0.0
2	0.0	0.0	0.0	1.3093295785560497	0.0
3	0.0	0.0	0.0	2.100589291434787	0.0
4	0.0	0.0	0.0	2.6066647296783856	0.0
5	0.0	0.0	0.0	5.4880092975437496	0.0
6	0.0	0.0	0.0	6.006898658263117	0.0
7	0.0	0.0	0.0	8.381348292842828	0.0
8	0.0	0.0	0.0	10.880212174716343	0.0
9	0.0	0.0	0.0	13.19647766131015	0.0
10	0.0	0.0	0.0	14.311512415349886	0.0
11	0.0	0.0	0.0	15.392575374918982	0.0
12	0.0	0.0	0.0	16.31428379858302	0.0
13	0.0	0.0	0.0	16.695274493589313	0.0
14	0.0	0.0	0.0	16.96570785746746	0.0
15	0.0	0.0	0.0	17.923250564334086	0.0
16	7.449954927772686E-5	0.0	0.0	18.926088997161568	0.0
17	7.449954927772686E-5	0.0	0.0	20.34605040639504	0.0
18	7.449954927772686E-5	0.0	0.0	20.968941137906114	0.0
19	7.449954927772686E-5	0.0	0.0	21.669907397060246	0.0
20	7.449954927772686E-5	0.0	0.0	22.51056031111012	0.0
21	7.449954927772686E-5	0.0	0.0	23.250266335888668	0.0
22	1.4899909855545373E-4	0.0	0.0	24.10231768097803	0.0
23	2.234986478331806E-4	0.0	0.0	24.723643921954274	0.0
24	2.234986478331806E-4	0.0	0.0	25.22197140707299	0.0
25	2.234986478331806E-4	0.0	0.0	25.712252940869707	0.0
26	2.234986478331806E-4	0.0	0.0	26.15850524104329	0.0
27	2.234986478331806E-4	0.0	0.0	26.614218983975146	0.0
28	2.234986478331806E-4	0.0	0.0	27.048923854010685	0.0
29	2.234986478331806E-4	0.0	0.0	27.62823234919429	0.0
30	2.234986478331806E-4	0.0	0.0	28.195844415141288	0.0
31	2.234986478331806E-4	0.0	0.0	28.717713757831763	0.0
32	2.234986478331806E-4	0.0	0.0	29.216190242049034	0.0
33	2.234986478331806E-4	0.0	0.0	29.66378353410962	0.0
34	2.234986478331806E-4	0.0	0.0	30.267527881456317	0.0
35	2.234986478331806E-4	0.0	0.0	30.747826475649823	0.0
36	2.234986478331806E-4	0.0	0.0	31.25487040803403	0.0
37	2.234986478331806E-4	0.0	0.0	31.73904297878998	0.0
38	2.234986478331806E-4	0.0	0.0	32.176280833500954	0.0
39	2.234986478331806E-4	0.0	0.0	32.69338220503766	0.0
40	2.234986478331806E-4	0.0	0.0	33.19722265680293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	40	5.6134013E-9	46.0	7
GATCGTA	25	3.4174634E-5	46.0	8
TTAATCG	200	0.0	46.0	19
CTATCGG	25	3.4174634E-5	46.0	1
ATATGCG	20	6.3118356E-4	46.0	18
TACGCGG	105	0.0	46.0	1
CGCCGAA	20	6.3118356E-4	46.0	42
TATCGGT	20	6.3118356E-4	46.0	38
AATACGG	50	1.6370905E-11	46.0	1
ATACCGG	60	0.0	46.0	1
TCGAACA	25	3.4174634E-5	46.0	33
TAGTAGG	265	0.0	45.132072	1
TAATAGG	145	0.0	44.41379	1
CCCGCAC	695	0.0	44.345325	32
CATGCGG	210	0.0	43.809525	1
ACGTAGG	105	0.0	43.809525	1
ATCAACG	815	0.0	43.742332	14
CGCACTT	700	0.0	43.37143	34
CGAATAT	240	0.0	43.125	14
GGCGATA	315	0.0	43.079365	7
>>END_MODULE