FastQCFastQC Report
Thu 26 May 2016
SRR1544973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544973_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences650647
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT30380.46691985054876145TruSeq Adapter, Index 27 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT22200.3411988374648619TruSeq Adapter, Index 27 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT21490.3302866223927875TruSeq Adapter, Index 27 (100% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA18550.2851008304041977No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.21363350634061173No Hit
TGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTTG13490.20733208636941383TruSeq Adapter, Index 27 (96% over 28bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8400.12910226282454232No Hit
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC8390.12894856965451312TruSeq Adapter, Index 27 (100% over 24bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT8280.12725794478419175TruSeq Adapter, Index 27 (96% over 26bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC7260.11158124144121158TruSeq Adapter, Index 27 (96% over 25bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGAAGG6980.10727783268039352No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6760.10389658293975075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTA206.309424E-446.00000426
CGACACG206.309424E-446.00000446
GTTACGA206.309424E-446.00000420
CCGTAGG405.6043064E-946.0000041
GTCGTAT206.309424E-446.00000427
GGTTACG206.309424E-446.00000419
AATACGG206.309424E-446.0000041
ATACCGG405.6043064E-946.0000041
TACGTAA206.309424E-446.00000440
GATGTTA301.8601477E-646.08
GATAACG253.41551E-546.08
CTATCGG253.41551E-546.01
CGTTGAT1200.046.024
CACGACC900.046.026
AGACACG850.046.023
GCGATAT253.41551E-546.08
GCGATAC453.092282E-1046.08
ATTGGGT253.41551E-546.03
TGTCCAC253.41551E-546.017
ATAGCGG1300.044.2307661
Produced by FastQC (version 0.11.5)