##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1544973_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 650647 Sequences flagged as poor quality 0 Sequence length 52 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.89928640261155 34.0 33.0 34.0 31.0 34.0 2 33.264052550768696 34.0 33.0 34.0 31.0 34.0 3 33.3945288305333 34.0 34.0 34.0 31.0 34.0 4 36.61198468601254 37.0 37.0 37.0 35.0 37.0 5 36.61918982182351 37.0 37.0 37.0 35.0 37.0 6 36.40934638905582 37.0 37.0 37.0 35.0 37.0 7 36.216496810098256 37.0 37.0 37.0 35.0 37.0 8 36.357995964017356 37.0 37.0 37.0 35.0 37.0 9 38.258147659176174 39.0 39.0 39.0 37.0 39.0 10 38.14441471335455 39.0 39.0 39.0 35.0 39.0 11 37.534203646524155 39.0 35.0 39.0 35.0 39.0 12 37.49707445050849 39.0 35.0 39.0 35.0 39.0 13 37.4844931276099 39.0 35.0 39.0 35.0 39.0 14 38.708084414436705 41.0 35.0 41.0 35.0 41.0 15 38.74946322660367 41.0 35.0 41.0 35.0 41.0 16 38.77333331284091 41.0 35.0 41.0 35.0 41.0 17 38.86307014402587 41.0 36.0 41.0 35.0 41.0 18 38.8705949616305 41.0 36.0 41.0 35.0 41.0 19 38.82865670632463 41.0 36.0 41.0 35.0 41.0 20 38.74682738873767 41.0 35.0 41.0 35.0 41.0 21 38.680485731894564 41.0 35.0 41.0 35.0 41.0 22 38.66312147754466 41.0 35.0 41.0 35.0 41.0 23 38.62404959985983 41.0 35.0 41.0 35.0 41.0 24 38.58767350037731 40.0 35.0 41.0 35.0 41.0 25 38.529087200893876 40.0 35.0 41.0 35.0 41.0 26 38.47489652607328 40.0 35.0 41.0 35.0 41.0 27 38.55415301999395 41.0 35.0 41.0 35.0 41.0 28 38.57534577120927 40.0 36.0 41.0 35.0 41.0 29 38.58051447251736 40.0 36.0 41.0 35.0 41.0 30 38.5165904092388 40.0 35.0 41.0 35.0 41.0 31 38.469315927069516 40.0 35.0 41.0 35.0 41.0 32 38.38626474878083 40.0 35.0 41.0 35.0 41.0 33 38.32198411734781 40.0 35.0 41.0 35.0 41.0 34 38.21630161977232 40.0 35.0 41.0 35.0 41.0 35 38.15268494283382 40.0 35.0 41.0 35.0 41.0 36 38.09560944721178 40.0 35.0 41.0 35.0 41.0 37 38.05899512331572 40.0 35.0 41.0 35.0 41.0 38 38.0110367065398 40.0 35.0 41.0 35.0 41.0 39 37.945260640562395 40.0 35.0 41.0 35.0 41.0 40 37.896436931239215 40.0 35.0 41.0 35.0 41.0 41 37.84750717362871 40.0 35.0 41.0 35.0 41.0 42 37.77633186658818 40.0 35.0 41.0 35.0 41.0 43 37.71207428913066 40.0 35.0 41.0 35.0 41.0 44 37.58187004627701 40.0 35.0 41.0 35.0 41.0 45 37.587545935046194 40.0 35.0 41.0 35.0 41.0 46 37.52051112200625 39.0 35.0 41.0 35.0 41.0 47 37.44776660769972 39.0 35.0 41.0 35.0 41.0 48 37.40652611938578 39.0 35.0 41.0 35.0 41.0 49 37.34028743696659 39.0 35.0 41.0 35.0 41.0 50 37.26766280333268 39.0 35.0 41.0 35.0 41.0 51 37.193822456723844 39.0 35.0 41.0 34.0 41.0 52 36.75994510079966 38.0 35.0 40.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 3.0 13 3.0 14 2.0 15 5.0 16 17.0 17 31.0 18 49.0 19 75.0 20 162.0 21 208.0 22 369.0 23 566.0 24 989.0 25 1655.0 26 2457.0 27 2975.0 28 2984.0 29 2965.0 30 3385.0 31 4264.0 32 5718.0 33 8355.0 34 30429.0 35 126209.0 36 24017.0 37 37893.0 38 65622.0 39 325948.0 40 3291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.6912334952747 21.961831838154943 22.961913295535062 21.385021371035293 2 27.89454189445275 23.950006685652898 25.753903422285816 22.401547997608535 3 28.214069994943497 24.09862798107115 24.546336185366258 23.1409658386191 4 26.164110493093794 25.97522158712789 22.633163604842565 25.22750431493575 5 23.87116208942791 35.599180508017405 20.797606075183626 19.732051327371064 6 85.68132950739802 3.204348901939147 7.700335204803833 3.413986385858999 7 85.40637242621575 3.086619933696767 6.357056898748477 5.149950741339006 8 73.50452703232321 6.234102362725103 12.29099650040652 7.970374104545168 9 50.01544616358794 19.750955587284658 15.100200262200547 15.133397986926859 10 40.216738108375196 20.22156407391412 25.186314545367917 14.37538327234276 11 38.455568073010404 17.451705763647567 24.178394736316314 19.914331427025715 12 21.05826969155318 30.922604730368384 28.31535379399275 19.703771784085685 13 15.389604501365564 32.950278722563844 30.52330987463248 21.136806901438106 14 14.788664206551324 24.001493897612683 41.526664996534215 19.68317689930177 15 16.718743035778232 17.554372801227085 44.71733520634076 21.009548956653916 16 16.98248051554837 20.877526523598817 28.6444108710253 33.49558208982751 17 19.586503895353395 23.754662666545762 31.917921699477596 24.740911738623247 18 24.02531633896721 27.56994191935105 27.06859479871574 21.336146942966003 19 27.640948163904543 22.605806220577364 25.04799069234162 24.70525492317647 20 21.03598418189894 30.03487298027963 26.980989691799085 21.948153146022346 21 20.854318854924404 23.44620047429712 25.685356268452786 30.014124402325688 22 19.933543073279367 28.71388018387851 23.594821769715377 27.757754973126747 23 19.562988840338924 22.156407391411932 36.004930476894536 22.275673291354607 24 19.898039951002616 21.916799739336383 32.02181828241735 26.16334202724365 25 17.352727362148755 28.8784855689798 29.184334977337944 24.584452091533503 26 18.48037415065312 27.91482939289661 26.967772079176573 26.6370243772737 27 20.043587383020288 27.310507848341725 32.704215957347074 19.941688811290913 28 18.44702273275678 25.05552165767305 28.93919437114134 27.558261238428827 29 19.53870531947431 25.782951431421342 27.049690538802146 27.62865271030221 30 24.903980192024246 25.954934088684034 23.47832234683323 25.662763372458492 31 26.08649544222904 23.159870098532693 24.167636214414266 26.585998244824 32 22.47916304847329 25.848424721853785 24.15503337447187 27.517378855201052 33 16.96542057367513 24.92841740605889 28.189325394568794 29.91683662569719 34 18.397533532007373 23.40531809106935 29.1099474830438 29.087200893879476 35 24.724005489920035 21.43635488982505 27.545658398486427 26.293981221768487 36 20.16439021466325 25.601286104446803 27.662618900878666 26.57170478001128 37 19.221482616534004 26.36206729609143 30.9484251829333 23.468024904441272 38 19.250069546159438 24.057591904673348 28.702506889296348 27.989831659870866 39 23.667057559629107 20.214801574432833 29.43024404938469 26.68789681655337 40 15.782136857620186 20.257067196190867 32.26572934325371 31.695066602935235 41 20.24876776500929 23.663522616718435 25.577617356262305 30.51009226200997 42 20.524339618871675 21.21088700939219 28.65916541534811 29.605607956388024 43 21.766026739537722 25.562248039259384 27.29990301960971 25.371822201593186 44 17.95781737255378 27.100562978081815 27.745613212694444 27.19600643666996 45 20.55308024166714 25.063513702514573 28.201467155001097 26.181938900817187 46 21.9747420644374 21.34260205610723 29.060458282294395 27.62219759716098 47 21.30110490019934 18.84939145189327 33.01867218322685 26.830831464680543 48 19.861153590195606 18.521410227050918 28.97070147099733 32.64673471175614 49 19.113282624833435 23.033534312768676 32.01213561270551 25.841047449692383 50 19.285418975266158 23.868395612367383 29.076903451487517 27.76928196087894 51 19.79137689100234 20.181142770196438 31.123635396766602 28.903844942034624 52 21.770944920978657 18.502659660307355 33.55644458515908 26.169950833554907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 90.0 1 236.5 2 383.0 3 1577.5 4 2772.0 5 1890.5 6 1009.0 7 1008.5 8 1008.0 9 1011.5 10 1015.0 11 965.0 12 915.0 13 873.0 14 860.0 15 889.0 16 806.0 17 723.0 18 801.0 19 879.0 20 989.5 21 1100.0 22 1152.0 23 1204.0 24 1405.5 25 1607.0 26 2061.5 27 2516.0 28 3410.0 29 4304.0 30 4611.0 31 4918.0 32 5535.5 33 6153.0 34 7281.5 35 8410.0 36 9637.5 37 10865.0 38 11762.5 39 14929.0 40 17198.0 41 23046.5 42 28895.0 43 37962.5 44 47030.0 45 55130.0 46 63230.0 47 69086.5 48 74943.0 49 76247.5 50 77552.0 51 68264.0 52 58976.0 53 52150.5 54 45325.0 55 39251.0 56 33177.0 57 30235.0 58 27293.0 59 26236.0 60 25179.0 61 25622.5 62 26066.0 63 23825.5 64 17503.5 65 13422.0 66 11108.0 67 8794.0 68 7181.0 69 5568.0 70 4942.0 71 4316.0 72 3628.0 73 2940.0 74 2572.5 75 2205.0 76 1759.0 77 1313.0 78 997.5 79 682.0 80 527.5 81 373.0 82 299.0 83 225.0 84 163.0 85 101.0 86 53.0 87 5.0 88 3.0 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 650647.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.34753521810283 #Duplication Level Percentage of deduplicated Percentage of total 1 70.8304685326986 18.66208264180001 2 11.200824432671999 5.902282324232242 3 4.111291363861926 3.249671820037023 4 1.9060157595820173 2.0087526940738485 5 1.1341529768250143 1.4941067749806611 6 0.8084199925297247 1.2779924534517209 7 0.5924649872268386 1.092699448151638 8 0.5190471951393345 1.0940491403032886 9 0.4269848022754296 1.01249974039919 >10 7.967914634309675 49.52917932666533 >50 0.3916142773435904 6.82460073970399 >100 0.09907606717225556 4.573932895097989 >500 0.008207484854506378 1.4244498567673123 >1k 0.003517493509074162 1.8537001443357748 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT 3038 0.46691985054876145 TruSeq Adapter, Index 27 (96% over 26bp) GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT 2220 0.3411988374648619 TruSeq Adapter, Index 27 (96% over 26bp) CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT 2149 0.3302866223927875 TruSeq Adapter, Index 27 (100% over 26bp) CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA 1855 0.2851008304041977 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1390 0.21363350634061173 No Hit TGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTTG 1349 0.20733208636941383 TruSeq Adapter, Index 27 (96% over 28bp) GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 840 0.12910226282454232 No Hit ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC 839 0.12894856965451312 TruSeq Adapter, Index 27 (100% over 24bp) TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT 828 0.12725794478419175 TruSeq Adapter, Index 27 (96% over 26bp) GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC 726 0.11158124144121158 TruSeq Adapter, Index 27 (96% over 25bp) GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGAAGG 698 0.10727783268039352 No Hit GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 676 0.10389658293975075 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.3376638945541899 0.0 2 0.0 0.0 0.0 1.3775518829718727 0.0 3 0.0 0.0 0.0 2.1469398921381333 0.0 4 0.0 0.0 0.0 2.638758036231628 0.0 5 1.5369317002921707E-4 0.0 0.0 5.400931687996717 0.0 6 1.5369317002921707E-4 0.0 0.0 5.892288752580124 0.0 7 1.5369317002921707E-4 0.0 0.0 8.21167238149104 0.0 8 1.5369317002921707E-4 0.0 0.0 10.616048333428111 0.0 9 1.5369317002921707E-4 0.0 0.0 12.914375998045022 0.0 10 1.5369317002921707E-4 0.0 0.0 14.001601482831704 0.0 11 1.5369317002921707E-4 0.0 0.0 15.041182084909329 0.0 12 3.0738634005843414E-4 0.0 0.0 15.984704455718692 0.0 13 4.610795100876512E-4 0.0 0.0 16.357256699869513 0.0 14 4.610795100876512E-4 0.0 0.0 16.62345327036012 0.0 15 4.610795100876512E-4 0.0 0.0 17.573738140650768 0.0 16 6.147726801168683E-4 0.0 0.0 18.508961080278553 0.0 17 6.147726801168683E-4 0.0 0.0 19.79153058417237 0.0 18 6.147726801168683E-4 0.0 0.0 20.39646690140737 0.0 19 6.147726801168683E-4 0.0 0.0 21.07963304218724 0.0 20 6.147726801168683E-4 0.0 0.0 21.879452299019285 0.0 21 6.147726801168683E-4 0.0 0.0 22.580293154352514 0.0 22 6.147726801168683E-4 0.0 0.0 23.377038547783975 0.0 23 6.147726801168683E-4 0.0 0.0 23.950160378822925 0.0 24 6.147726801168683E-4 0.0 0.0 24.42215210398265 0.0 25 6.147726801168683E-4 0.0 0.0 24.90136740813375 0.0 26 6.147726801168683E-4 0.0 0.0 25.334782147616142 0.0 27 7.684658501460853E-4 0.0 0.0 25.768043193928506 0.0 28 7.684658501460853E-4 0.0 0.0 26.195463899779757 0.0 29 7.684658501460853E-4 0.0 0.0 26.739691414853215 0.0 30 7.684658501460853E-4 0.0 0.0 27.27285302168457 0.0 31 7.684658501460853E-4 0.0 0.0 27.760214063847215 0.0 32 7.684658501460853E-4 0.0 0.0 28.235279652407527 0.0 33 7.684658501460853E-4 0.0 0.0 28.631500644742847 0.0 34 7.684658501460853E-4 0.0 0.0 29.189560545118937 0.0 35 7.684658501460853E-4 0.0 0.0 29.65202329373685 0.0 36 7.684658501460853E-4 0.0 0.0 30.108492008723623 0.0 37 7.684658501460853E-4 0.0 0.0 30.5623479398199 0.0 38 7.684658501460853E-4 0.0 0.0 30.97916381693914 0.0 39 7.684658501460853E-4 0.0 0.0 31.473902131263188 0.0 40 7.684658501460853E-4 0.0 0.0 31.965259195846595 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCGTA 20 6.309424E-4 46.000004 26 CGACACG 20 6.309424E-4 46.000004 46 GTTACGA 20 6.309424E-4 46.000004 20 CCGTAGG 40 5.6043064E-9 46.000004 1 GTCGTAT 20 6.309424E-4 46.000004 27 GGTTACG 20 6.309424E-4 46.000004 19 AATACGG 20 6.309424E-4 46.000004 1 ATACCGG 40 5.6043064E-9 46.000004 1 TACGTAA 20 6.309424E-4 46.000004 40 GATGTTA 30 1.8601477E-6 46.0 8 GATAACG 25 3.41551E-5 46.0 8 CTATCGG 25 3.41551E-5 46.0 1 CGTTGAT 120 0.0 46.0 24 CACGACC 90 0.0 46.0 26 AGACACG 85 0.0 46.0 23 GCGATAT 25 3.41551E-5 46.0 8 GCGATAC 45 3.092282E-10 46.0 8 ATTGGGT 25 3.41551E-5 46.0 3 TGTCCAC 25 3.41551E-5 46.0 17 ATAGCGG 130 0.0 44.230766 1 >>END_MODULE