FastQCFastQC Report
Thu 26 May 2016
SRR1544972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544972_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1011232
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT30820.3047767475712794Illumina Single End Adapter 1 (95% over 22bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAGAGG24270.24000427201670832No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT23650.23387313692604667Illumina Single End Adapter 1 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT22080.21834752064808075No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT21250.21013971076864657Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.18502183475206482No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA12890.1274682763203696No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11290.11164599221543622No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG10720.10600930350305371No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10590.10472374291952787No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10190.10076817189329451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACC351.01939804E-746.00000413
AATCCCG206.3110935E-446.09
TCGCAAA206.3110935E-446.019
CATATTA453.092282E-1046.028
CGTATAT551.8189894E-1246.029
GCACGTA206.3110935E-446.09
CGACCGT206.3110935E-446.021
CAATTGG301.861179E-646.01
TACGCGT253.4168617E-546.021
GATATAC206.3110935E-446.08
GTCCGTT206.3110935E-446.08
CGAATAT1600.046.014
TCCCGAA253.4168617E-546.031
ACCGTAG206.3110935E-446.025
CACACGG650.046.01
TTACCCG206.3110935E-446.010
CGTTTAG253.4168617E-546.038
AGTACGG850.046.01
ACGCGTA253.4168617E-546.022
TAACGGG2550.045.098042
Produced by FastQC (version 0.11.5)