##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544972_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1011232
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95940199677226	34.0	33.0	34.0	31.0	34.0
2	33.26907475238125	34.0	33.0	34.0	31.0	34.0
3	33.330480048099744	34.0	34.0	34.0	31.0	34.0
4	36.59919385462485	37.0	37.0	37.0	35.0	37.0
5	36.60197363216354	37.0	37.0	37.0	35.0	37.0
6	36.67850898705737	37.0	37.0	37.0	35.0	37.0
7	36.52643705895383	37.0	37.0	37.0	35.0	37.0
8	36.54646114838138	37.0	37.0	37.0	35.0	37.0
9	38.34055290971805	39.0	39.0	39.0	37.0	39.0
10	38.1411189519319	39.0	39.0	39.0	35.0	39.0
11	37.466774192272396	39.0	35.0	39.0	35.0	39.0
12	37.38000775291921	39.0	35.0	39.0	35.0	39.0
13	37.37091785070093	39.0	35.0	39.0	35.0	39.0
14	38.671121958165884	41.0	35.0	41.0	35.0	41.0
15	38.725446781747415	41.0	35.0	41.0	35.0	41.0
16	38.71560532103415	41.0	35.0	41.0	35.0	41.0
17	38.77983390557261	41.0	36.0	41.0	35.0	41.0
18	38.78208066991551	41.0	36.0	41.0	35.0	41.0
19	38.70640367393437	41.0	35.0	41.0	35.0	41.0
20	38.616476733331226	41.0	35.0	41.0	35.0	41.0
21	38.56935599348122	41.0	35.0	41.0	35.0	41.0
22	38.535119537356415	41.0	35.0	41.0	35.0	41.0
23	38.51373473149584	40.0	35.0	41.0	35.0	41.0
24	38.45058700674029	40.0	35.0	41.0	35.0	41.0
25	38.408300963577105	40.0	35.0	41.0	35.0	41.0
26	38.35428467453561	40.0	35.0	41.0	35.0	41.0
27	38.33606531438878	40.0	35.0	41.0	35.0	41.0
28	38.361640058858896	40.0	35.0	41.0	35.0	41.0
29	38.3660969985127	40.0	36.0	41.0	35.0	41.0
30	38.28818510490174	40.0	35.0	41.0	35.0	41.0
31	38.22779144647321	40.0	35.0	41.0	35.0	41.0
32	38.14642831714186	40.0	35.0	41.0	35.0	41.0
33	38.07117160058226	40.0	35.0	41.0	35.0	41.0
34	37.95368520774659	40.0	35.0	41.0	35.0	41.0
35	37.90271371950255	40.0	35.0	41.0	35.0	41.0
36	37.83172605297301	40.0	35.0	41.0	35.0	41.0
37	37.78760858042467	40.0	35.0	41.0	35.0	41.0
38	37.720911719565834	40.0	35.0	41.0	35.0	41.0
39	37.60563846871934	40.0	35.0	41.0	35.0	41.0
40	37.543442058795605	40.0	35.0	41.0	34.0	41.0
41	37.51166102338534	39.0	35.0	41.0	34.0	41.0
42	37.42367725704883	39.0	35.0	41.0	35.0	41.0
43	37.339834973576785	39.0	35.0	41.0	34.0	41.0
44	37.26342026359925	39.0	35.0	41.0	34.0	41.0
45	37.22156241099965	39.0	35.0	41.0	34.0	41.0
46	37.225395359324075	39.0	35.0	41.0	34.0	41.0
47	37.182877915255844	39.0	35.0	41.0	34.0	41.0
48	37.17155805987152	38.0	35.0	41.0	34.0	41.0
49	37.111282079681025	38.0	35.0	41.0	34.0	41.0
50	37.027016550109174	38.0	35.0	41.0	34.0	41.0
51	36.94553376475428	38.0	35.0	41.0	34.0	41.0
52	36.74839008259232	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	7.0
14	14.0
15	17.0
16	26.0
17	50.0
18	107.0
19	174.0
20	274.0
21	420.0
22	700.0
23	1119.0
24	1732.0
25	2878.0
26	4056.0
27	4813.0
28	5171.0
29	5654.0
30	6386.0
31	8192.0
32	10458.0
33	15218.0
34	50240.0
35	206806.0
36	37349.0
37	58625.0
38	102872.0
39	483521.0
40	4349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.96240229739565	21.60750450935097	24.124533242618902	19.305559950634475
2	27.586547894053986	24.009426125755514	25.251178760165814	23.152847220024682
3	27.732607354197654	24.611365146672572	25.426311667352298	22.229715831777476
4	26.644726432707827	26.591622891680643	22.009192747064965	24.754457928546564
5	23.93258915857093	36.50369054776748	19.940725768171895	19.622994525489702
6	88.20033385019461	3.1615890319926585	6.342263694186894	2.2958134236258347
7	88.10441125280846	2.972809404765672	4.992820638587387	3.9299587038384862
8	77.7730530679409	5.536118319040536	10.101242840416443	6.589585772602133
9	51.489766937755135	21.45086389671213	13.95950681940445	13.099862346128289
10	41.67619300022151	22.245637005158063	22.540425935888106	13.537744058732319
11	41.32553162874593	17.88105993481219	22.94498196259612	17.848426473845763
12	22.482575709629444	32.904318692446445	25.77450080693649	18.838604790987628
13	15.73160263915699	35.37219945571343	27.775723078383596	21.120474826745987
14	14.978758583589128	24.404587671276225	42.02082212588209	18.595831619252557
15	17.70375146356128	18.171398848137716	44.598667763678364	19.526181924622637
16	16.826900256321004	20.405208695927342	28.149821208189614	34.61806983956204
17	19.67164804911237	23.834194329293375	31.2067853865384	25.287372235055855
18	24.80914369798424	26.944064270118034	26.178463497990567	22.068328533907156
19	28.701920034176133	22.880802822695482	24.698486598525363	23.718790544603017
20	23.35824024556185	28.621918610170567	26.253124901110723	21.76671624315686
21	21.664761399955697	24.362658618398154	24.45630676244423	29.51627321920192
22	20.976986487769373	28.19313471092687	23.89422011961647	26.935658681687286
23	21.143120470871175	21.856408816176703	35.43608192778709	21.564388785165026
24	21.02336555805196	21.733786114363472	31.882891364197334	25.359956963387237
25	18.153895446346635	30.08666656118477	27.433269516787444	24.326168475681147
26	19.059226764975794	29.83370779405715	26.975807727603556	24.131257713363503
27	19.77844846682067	28.199661403120153	31.95468497832347	20.067205151735703
28	18.641419575329895	25.797838675991265	29.338173633745768	26.222568114933072
29	21.0774579918357	26.850119458244993	26.687446599791148	25.384975950128158
30	25.913638017784248	27.00349672478719	22.590463909369955	24.492401348058607
31	29.20872757191228	25.624881332869215	22.463490079427867	22.702901015790637
32	25.620925761842976	28.267993892598337	21.179215056485553	24.93186528907313
33	20.888381696781746	28.874185152368597	24.370569760450618	25.866863390399036
34	20.760616752634412	26.757855764058096	26.494711401537923	25.986816081769565
35	29.275181165153004	25.627749121863232	21.819325337805765	23.277744375178
36	21.598208917439322	32.30752191386348	23.897582354988767	22.19668681370843
37	23.465831777475398	29.37298265877662	25.06951916078605	22.09166640296193
38	22.18046897250087	29.20576089364261	24.060255213442613	24.55351492041391
39	26.872863991645836	24.27326271320528	25.868841175912156	22.985032119236735
40	17.20722841049334	25.07426584601753	29.775857567798486	27.942648175690643
41	22.056659599379767	27.20463751147116	22.855586057403247	27.88311683174583
42	22.918479636720356	24.38836983006867	25.332960191133193	27.360190342077782
43	22.867254991930636	28.331777475396347	25.789630866111835	23.011336666561185
44	18.92513290718648	30.868089617417173	23.853477738046262	26.353299737350085
45	22.999469953482485	28.91997088699725	23.93051248378216	24.15004667573811
46	23.5379220594285	25.641000284801112	25.638132495807092	25.182945159963293
47	23.357943577734883	22.198862377772855	29.741740767697223	24.70145327679504
48	21.433657162748016	22.86339831018006	27.132349450966743	28.57059507610519
49	20.924080725293503	26.650857567798486	28.05548083921395	24.36958086769406
50	19.84776984905541	27.31252571121167	26.16610233853359	26.67360210119933
51	20.89283171418626	23.804527546596628	28.672154362203727	26.630486377013384
52	23.70455048890858	21.448589443372047	30.15094459036106	24.695915477358312
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97.0
1	318.0
2	539.0
3	2115.5
4	3692.0
5	2468.5
6	1245.0
7	1302.0
8	1359.0
9	1403.0
10	1447.0
11	1368.5
12	1290.0
13	1281.5
14	1248.0
15	1223.0
16	1235.0
17	1247.0
18	1346.0
19	1445.0
20	1471.0
21	1497.0
22	1854.0
23	2211.0
24	2541.0
25	2871.0
26	3246.5
27	3622.0
28	6419.0
29	9216.0
30	9282.0
31	9348.0
32	10681.5
33	12015.0
34	14251.5
35	16488.0
36	18189.0
37	19890.0
38	22129.5
39	27408.5
40	30448.0
41	39978.5
42	49509.0
43	64568.0
44	79627.0
45	86606.5
46	93586.0
47	97252.0
48	100918.0
49	101292.5
50	101667.0
51	92486.0
52	83305.0
53	74120.5
54	64936.0
55	57865.0
56	50794.0
57	47210.0
58	43626.0
59	43468.5
60	43311.0
61	45064.0
62	46817.0
63	42521.5
64	30559.0
65	22892.0
66	18812.0
67	14732.0
68	12069.5
69	9407.0
70	8071.5
71	6736.0
72	6079.0
73	5422.0
74	4798.0
75	4174.0
76	3479.5
77	2785.0
78	1868.5
79	952.0
80	725.0
81	498.0
82	430.0
83	362.0
84	224.0
85	86.0
86	55.0
87	24.0
88	15.5
89	3.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1011232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.665534145113448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.87550155354823	15.13890064815327
2	11.962656154571553	5.18354670766243
3	4.5944125204122255	2.9862120401318344
4	2.1808253669517272	1.8899498580888883
5	1.3062893917333653	1.415072870999935
6	0.8240563486857067	1.0712172575968613
7	0.5781258544242688	0.8767783807441315
8	0.4546819332816027	0.7880741560543006
9	0.35123037531183604	0.6848634320207634
>10	5.77543862112733	33.23103917196929
>50	1.8574107024128046	25.967141545419352
>100	0.2265803512586554	7.63861715957061
>500	0.0077658588133006886	1.1841934997960915
>1k	0.005024967467429857	1.9443932717922285
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	3082	0.3047767475712794	Illumina Single End Adapter 1 (95% over 22bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAGAGG	2427	0.24000427201670832	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	2365	0.23387313692604667	Illumina Single End Adapter 1 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	2208	0.21834752064808075	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT	2125	0.21013971076864657	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1871	0.18502183475206482	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1289	0.1274682763203696	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1129	0.11164599221543622	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	1072	0.10600930350305371	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1059	0.10472374291952787	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1019	0.10076817189329451	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21528195310274992	0.0
2	0.0	0.0	0.0	0.9246147273820449	0.0
3	0.0	0.0	0.0	1.5009414259042435	0.0
4	0.0	0.0	0.0	1.8503172367963039	0.0
5	0.0	0.0	0.0	3.867065124521376	0.0
6	0.0	0.0	0.0	4.219407613683111	0.0
7	0.0	0.0	0.0	5.799064903009398	0.0
8	0.0	0.0	0.0	7.539615043827727	0.0
9	0.0	0.0	0.0	9.013361918926616	0.0
10	0.0	0.0	0.0	9.827715103952407	0.0
11	0.0	0.0	0.0	10.614873738172843	0.0
12	0.0	0.0	0.0	11.314119806335242	0.0
13	0.0	0.0	0.0	11.578747507990254	0.0
14	0.0	0.0	0.0	11.795809468054808	0.0
15	0.0	0.0	0.0	12.533325685896015	0.0
16	0.0	0.0	0.0	13.298036454542578	0.0
17	0.0	0.0	0.0	14.346064997943103	0.0
18	0.0	0.0	0.0	14.799670105376412	0.0
19	0.0	0.0	0.0	15.362745640960728	0.0
20	0.0	0.0	0.0	16.03993940065188	0.0
21	0.0	0.0	0.0	16.653052909718046	0.0
22	0.0	0.0	0.0	17.37138460808202	0.0
23	0.0	0.0	0.0	17.891443308756052	0.0
24	0.0	0.0	0.0	18.322402772064176	0.0
25	0.0	0.0	0.0	18.724486566880795	0.0
26	0.0	0.0	0.0	19.103034714091326	0.0
27	0.0	0.0	0.0	19.509865194139426	0.0
28	0.0	0.0	0.0	19.894247808613652	0.0
29	0.0	0.0	0.0	20.390671972405936	0.0
30	0.0	0.0	0.0	20.872757191228125	0.0
31	0.0	0.0	0.0	21.34881016423531	0.0
32	0.0	0.0	0.0	21.763354007784564	0.0
33	0.0	0.0	0.0	22.17147004841619	0.0
34	0.0	0.0	0.0	22.71832774279295	0.0
35	0.0	0.0	0.0	23.11685152368596	0.0
36	0.0	0.0	0.0	23.59745340337331	0.0
37	0.0	0.0	0.0	24.018029492737572	0.0
38	0.0	0.0	0.0	24.43435334324863	0.0
39	9.888927565583367E-5	0.0	0.0	24.88064064428341	0.0
40	9.888927565583367E-5	0.0	0.0	25.367472548337076	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTACC	35	1.01939804E-7	46.000004	13
AATCCCG	20	6.3110935E-4	46.0	9
TCGCAAA	20	6.3110935E-4	46.0	19
CATATTA	45	3.092282E-10	46.0	28
CGTATAT	55	1.8189894E-12	46.0	29
GCACGTA	20	6.3110935E-4	46.0	9
CGACCGT	20	6.3110935E-4	46.0	21
CAATTGG	30	1.861179E-6	46.0	1
TACGCGT	25	3.4168617E-5	46.0	21
GATATAC	20	6.3110935E-4	46.0	8
GTCCGTT	20	6.3110935E-4	46.0	8
CGAATAT	160	0.0	46.0	14
TCCCGAA	25	3.4168617E-5	46.0	31
ACCGTAG	20	6.3110935E-4	46.0	25
CACACGG	65	0.0	46.0	1
TTACCCG	20	6.3110935E-4	46.0	10
CGTTTAG	25	3.4168617E-5	46.0	38
AGTACGG	85	0.0	46.0	1
ACGCGTA	25	3.4168617E-5	46.0	22
TAACGGG	255	0.0	45.09804	2
>>END_MODULE