FastQCFastQC Report
Thu 26 May 2016
SRR1544971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544971_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences635181
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT26720.4206674947770793Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT19900.31329652492754034Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA19300.3038503985478155No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT18020.28369866227106916Illumina Single End Adapter 1 (95% over 23bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAGAGG14540.22891112926866514No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.20372145892273227No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTTG11240.17695743418017856Illumina Single End Adapter 1 (95% over 24bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT9270.1459426525667487No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG7310.1150853063929809No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT7260.1142981291946705Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC7200.11335351655669801No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC6950.109417630565146Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC6560.10327764841832486Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTC206.309312E-446.036
TCGTTCA206.309312E-446.015
CGTATAT253.4154178E-546.029
CTATCGG206.309312E-446.01
GTCCTAG206.309312E-446.042
TAAACGG253.4154178E-546.01
TAACGTA206.309312E-446.024
ACGGGAT1300.046.04
ACGGGAC850.046.04
CTAACGG253.4154178E-546.01
AACATAG206.309312E-446.014
TCCACGT206.309312E-446.011
GATATAC206.309312E-446.08
CGAATAT600.046.014
TCGTATA253.4154178E-546.028
CGTACGA206.309312E-446.021
GATTCGC206.309312E-446.08
ATCGAGT206.309312E-446.023
GTACGAT206.309312E-446.09
AGTACGG351.0185977E-745.9999961
Produced by FastQC (version 0.11.5)