##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544971_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	635181
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94174888732503	34.0	33.0	34.0	31.0	34.0
2	33.29577710920194	34.0	34.0	34.0	31.0	34.0
3	33.42253153038268	34.0	34.0	34.0	31.0	34.0
4	36.63456243181078	37.0	37.0	37.0	35.0	37.0
5	36.64103617708968	37.0	37.0	37.0	35.0	37.0
6	36.440194212358364	37.0	37.0	37.0	35.0	37.0
7	36.273896731797706	37.0	37.0	37.0	35.0	37.0
8	36.40175005234728	37.0	37.0	37.0	35.0	37.0
9	38.28197159549798	39.0	39.0	39.0	37.0	39.0
10	38.158068330129524	39.0	39.0	39.0	35.0	39.0
11	37.54053883853579	39.0	35.0	39.0	35.0	39.0
12	37.50734987350062	39.0	35.0	39.0	35.0	39.0
13	37.498771216393436	39.0	35.0	39.0	35.0	39.0
14	38.72546250596287	41.0	35.0	41.0	35.0	41.0
15	38.75837123591543	41.0	35.0	41.0	35.0	41.0
16	38.76796062854525	41.0	35.0	41.0	35.0	41.0
17	38.862812332232856	41.0	36.0	41.0	35.0	41.0
18	38.88896550746952	41.0	36.0	41.0	35.0	41.0
19	38.843824043855214	41.0	36.0	41.0	35.0	41.0
20	38.764574192238115	41.0	35.0	41.0	35.0	41.0
21	38.71825353718074	41.0	35.0	41.0	35.0	41.0
22	38.68227639050916	41.0	35.0	41.0	35.0	41.0
23	38.64453911562216	41.0	35.0	41.0	35.0	41.0
24	38.587901716203724	41.0	35.0	41.0	35.0	41.0
25	38.54472347252201	40.0	35.0	41.0	35.0	41.0
26	38.468581396483835	40.0	35.0	41.0	35.0	41.0
27	38.56181308949733	41.0	35.0	41.0	35.0	41.0
28	38.563656658495766	40.0	36.0	41.0	35.0	41.0
29	38.582874802615315	40.0	36.0	41.0	35.0	41.0
30	38.51967549407177	40.0	35.0	41.0	35.0	41.0
31	38.46558540006707	40.0	35.0	41.0	35.0	41.0
32	38.40501841207467	40.0	35.0	41.0	35.0	41.0
33	38.333928439295256	40.0	35.0	41.0	35.0	41.0
34	38.223581939636105	40.0	35.0	41.0	35.0	41.0
35	38.171384219616144	40.0	35.0	41.0	35.0	41.0
36	38.12089152540772	40.0	35.0	41.0	35.0	41.0
37	38.06524754361355	40.0	35.0	41.0	35.0	41.0
38	38.001026479066596	40.0	35.0	41.0	35.0	41.0
39	37.93224923289582	40.0	35.0	41.0	35.0	41.0
40	37.872522950151215	40.0	35.0	41.0	35.0	41.0
41	37.83297359335371	40.0	35.0	41.0	35.0	41.0
42	37.76533303105729	40.0	35.0	41.0	35.0	41.0
43	37.700304322704866	40.0	35.0	41.0	35.0	41.0
44	37.565519119746966	39.0	35.0	41.0	35.0	41.0
45	37.561780028055	39.0	35.0	41.0	35.0	41.0
46	37.49101122357249	39.0	35.0	41.0	35.0	41.0
47	37.411904638205485	39.0	35.0	41.0	35.0	41.0
48	37.35293247121687	39.0	35.0	41.0	35.0	41.0
49	37.29341085454382	39.0	35.0	41.0	35.0	41.0
50	37.22067410706555	39.0	35.0	41.0	35.0	41.0
51	37.13548736501879	38.0	35.0	41.0	34.0	41.0
52	36.66592357139146	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	1.0
15	9.0
16	8.0
17	21.0
18	37.0
19	83.0
20	151.0
21	247.0
22	435.0
23	581.0
24	954.0
25	1572.0
26	2422.0
27	2792.0
28	2610.0
29	2923.0
30	3179.0
31	4076.0
32	5363.0
33	7944.0
34	29537.0
35	124892.0
36	22129.0
37	36854.0
38	65949.0
39	317693.0
40	2713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.13672638192893	21.286216054951264	23.437886208812923	21.13917135430688
2	27.431078700401933	24.15673642630998	25.19644006983836	23.215744803449727
3	27.850959017980703	24.51757845401547	25.449281385935663	22.182181142068167
4	26.587382179252845	26.048165798410217	22.56301747061074	24.8014345517262
5	23.896653079988223	35.666683984565026	20.26414518066504	20.17251775478171
6	86.7215801480208	3.105414047334539	7.207079556850725	2.965926247793936
7	86.78974969339448	2.8280128026499534	5.713332105336904	4.668905398618661
8	75.7329013304869	5.496071198603233	11.387777657077274	7.3832498138325935
9	50.74301655748519	20.62278311221526	14.614574428391277	14.019625901908276
10	40.42265118131682	21.7805633354902	23.97726002509521	13.81952545809777
11	39.69876302975058	17.706291592475214	23.519122895678553	19.075822482095656
12	22.291126466314324	31.266520881449537	26.974988231700888	19.467364420535247
13	15.689386174964302	33.904823979306684	28.519429894785898	21.886359950943117
14	14.945503722560971	23.978519508612507	41.319560881071695	19.75641588775483
15	17.610728280600334	18.024153745152958	44.08727591033107	20.277842063915642
16	16.538907807380888	20.82571739393968	28.632311105023607	34.00306369365582
17	19.127146435425495	23.75259965269742	31.459064424156264	25.66118948772082
18	24.371006059690075	27.0272567976687	26.50157986463701	22.10015727800422
19	27.82608421851409	22.902448278522186	24.767585932198855	24.503881570764868
20	22.82798131556202	29.122879935010648	26.227169893306	21.821968856121327
21	21.227807506836633	24.219868037614475	24.70461175633402	29.847712699214867
22	20.575709915756295	28.431423484014793	24.048105972943144	26.944760627285763
23	20.71441053809859	21.917847038875532	35.2889963648157	22.07874605821018
24	21.11996423066811	21.842278027837732	31.25849167402677	25.779266067467383
25	18.118457573510543	29.74632427607249	27.47862420317988	24.656593947237088
26	19.169496568694594	28.49156382196571	26.856911651954324	25.482027957385373
27	20.584683735817038	27.441941745738617	31.379402091687254	20.593972426757098
28	19.17925756595364	25.694408365489522	28.78990398012535	26.336430088431484
29	21.566293702110105	25.95323222829398	26.009279244813683	26.47119482478223
30	25.682915578394187	27.25978894204959	21.763717743446357	25.293577736109864
31	29.550789460012183	25.24571736245259	21.477657549580357	23.725835627954865
32	25.057267141177082	27.861192321558736	21.178687649662066	25.90285288760212
33	20.679144999614284	28.84721048016235	24.280638117324038	26.193006402899332
34	21.05195212073409	26.681213701291444	26.293922519722724	25.97291165825174
35	29.14082757513213	25.421415313115475	21.708457904124966	23.729299207627434
36	21.80433608687917	32.63935791530288	22.758709722110705	22.79759627570724
37	23.86390650853851	29.633915372153762	24.841265717960706	21.66091240134702
38	22.280105985537983	29.47490557809506	23.00116029919031	25.24382813717665
39	27.303555994275648	24.073610514168404	25.32191611524904	23.300917376306913
40	17.40023709777213	24.664308283780528	29.378240218142544	28.557214400304794
41	21.949963868566595	27.391090098727766	22.382596456757994	28.276349575947645
42	22.484929492538345	23.89696795086755	25.60624451927876	28.011858037315346
43	23.69198700842752	27.746107015165755	25.465812107100184	23.096093869306543
44	19.26836602480238	29.58369346690156	24.429414607804706	26.718525900491358
45	22.72281444186775	28.134657680251774	24.173582018353823	24.968945859526652
46	23.806914879380837	24.51395743890324	25.612699372304903	26.066428309411016
47	22.85128176063201	21.479231903976977	30.34268972151245	25.326796613878567
48	21.15948052602329	22.018605720259266	27.00993889930587	29.811974854411577
49	20.731885871901078	25.45652341616012	28.55485286870986	25.256737843228937
50	19.85497047298329	26.339421361785064	26.868247003609998	26.93736116162165
51	21.062972601510435	22.931888705738995	28.409067651582774	27.59607104116779
52	23.579735539948455	20.995905104214387	30.624184287628253	24.800175068208905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	192.5
2	330.0
3	1368.0
4	2406.0
5	1622.5
6	839.0
7	863.0
8	887.0
9	923.5
10	960.0
11	934.5
12	909.0
13	858.5
14	819.0
15	830.0
16	817.0
17	804.0
18	834.0
19	864.0
20	888.0
21	912.0
22	1091.5
23	1271.0
24	1449.5
25	1628.0
26	1831.5
27	2035.0
28	3386.0
29	4737.0
30	4858.5
31	4980.0
32	5761.0
33	6542.0
34	7872.5
35	9203.0
36	10204.0
37	11205.0
38	12697.5
39	15744.0
40	17298.0
41	22774.5
42	28251.0
43	37374.0
44	46497.0
45	51848.5
46	57200.0
47	61611.5
48	66023.0
49	67387.0
50	68751.0
51	62394.5
52	56038.0
53	49522.5
54	43007.0
55	38193.0
56	33379.0
57	30633.0
58	27887.0
59	27417.5
60	26948.0
61	28146.0
62	29344.0
63	27213.0
64	19746.5
65	14411.0
66	11825.5
67	9240.0
68	7743.0
69	6246.0
70	5230.5
71	4215.0
72	3826.5
73	3438.0
74	3009.0
75	2580.0
76	2159.5
77	1739.0
78	1193.0
79	647.0
80	476.0
81	305.0
82	249.5
83	194.0
84	121.0
85	48.0
86	31.0
87	14.0
88	8.5
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	635181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.28740935490362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.04493639042903	18.675873254882966
2	11.038778633546297	5.803617854363902
3	3.9911976849807815	3.147547420843004
4	1.8198731809513955	1.9135900512671968
5	1.1366379388327876	1.4939633393205694
6	0.7777650417130516	1.2267256820066805
7	0.6034309330312593	1.1103845167802902
8	0.5149355725045563	1.0829057748663138
9	0.45106022625711467	1.0671484330202596
>10	8.142503992595685	50.67135161529721
>50	0.37359890904915666	6.491946748766184
>100	0.09385093367418426	4.154055182700154
>500	0.007219302590321866	1.2210553190553886
>1k	0.004211259844354422	1.939834806829866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	2672	0.4206674947770793	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	1990	0.31329652492754034	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1930	0.3038503985478155	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT	1802	0.28369866227106916	Illumina Single End Adapter 1 (95% over 23bp)
GGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAGAGG	1454	0.22891112926866514	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1294	0.20372145892273227	No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTTG	1124	0.17695743418017856	Illumina Single End Adapter 1 (95% over 24bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	927	0.1459426525667487	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	731	0.1150853063929809	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	726	0.1142981291946705	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	720	0.11335351655669801	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	695	0.109417630565146	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	656	0.10327764841832486	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2882642900212695	0.0
2	0.0	0.0	0.0	1.2276815584849043	0.0
3	0.0	0.0	0.0	1.9372430850419013	0.0
4	0.0	0.0	0.0	2.369875673233299	0.0
5	0.0	0.0	0.0	4.87609043721396	0.0
6	0.0	0.0	0.0	5.2813292589041545	0.0
7	0.0	0.0	0.0	7.230065131041388	0.0
8	0.0	0.0	0.0	9.258778206526959	0.0
9	0.0	0.0	0.0	11.047402236527855	0.0
10	0.0	0.0	0.0	12.065537224822531	0.0
11	0.0	0.0	0.0	12.978505339422936	0.0
12	0.0	0.0	0.0	13.810866508916357	0.0
13	0.0	0.0	0.0	14.158956266009216	0.0
14	0.0	0.0	0.0	14.409278615071925	0.0
15	0.0	0.0	0.0	15.25643871589358	0.0
16	0.0	0.0	0.0	16.132409502173395	0.0
17	0.0	0.0	0.0	17.2571282831193	0.0
18	1.5743543966208057E-4	0.0	0.0	17.78973237549612	0.0
19	1.5743543966208057E-4	0.0	0.0	18.408296217928434	0.0
20	1.5743543966208057E-4	0.0	0.0	19.120534146959685	0.0
21	1.5743543966208057E-4	0.0	0.0	19.811360856196895	0.0
22	1.5743543966208057E-4	0.0	0.0	20.58657296109298	0.0
23	1.5743543966208057E-4	0.0	0.0	21.170343571359975	0.0
24	1.5743543966208057E-4	0.0	0.0	21.6626441911833	0.0
25	1.5743543966208057E-4	0.0	0.0	22.10881622718564	0.0
26	1.5743543966208057E-4	0.0	0.0	22.527909367566096	0.0
27	1.5743543966208057E-4	0.0	0.0	22.960699391197156	0.0
28	1.5743543966208057E-4	0.0	0.0	23.357121828266273	0.0
29	1.5743543966208057E-4	0.0	0.0	23.88421568025492	0.0
30	1.5743543966208057E-4	0.0	0.0	24.390213183328846	0.0
31	1.5743543966208057E-4	0.0	0.0	24.877003562764	0.0
32	1.5743543966208057E-4	0.0	0.0	25.309163844636412	0.0
33	1.5743543966208057E-4	0.0	0.0	25.720542648473426	0.0
34	1.5743543966208057E-4	0.0	0.0	26.271409251851047	0.0
35	1.5743543966208057E-4	0.0	0.0	26.700893131249202	0.0
36	1.5743543966208057E-4	0.0	0.0	27.179339432382267	0.0
37	1.5743543966208057E-4	0.0	0.0	27.612916633211636	0.0
38	1.5743543966208057E-4	0.0	0.0	28.025082614246962	0.0
39	1.5743543966208057E-4	0.0	0.0	28.489359725810438	0.0
40	1.5743543966208057E-4	0.0	0.0	28.982604958271736	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTC	20	6.309312E-4	46.0	36
TCGTTCA	20	6.309312E-4	46.0	15
CGTATAT	25	3.4154178E-5	46.0	29
CTATCGG	20	6.309312E-4	46.0	1
GTCCTAG	20	6.309312E-4	46.0	42
TAAACGG	25	3.4154178E-5	46.0	1
TAACGTA	20	6.309312E-4	46.0	24
ACGGGAT	130	0.0	46.0	4
ACGGGAC	85	0.0	46.0	4
CTAACGG	25	3.4154178E-5	46.0	1
AACATAG	20	6.309312E-4	46.0	14
TCCACGT	20	6.309312E-4	46.0	11
GATATAC	20	6.309312E-4	46.0	8
CGAATAT	60	0.0	46.0	14
TCGTATA	25	3.4154178E-5	46.0	28
CGTACGA	20	6.309312E-4	46.0	21
GATTCGC	20	6.309312E-4	46.0	8
ATCGAGT	20	6.309312E-4	46.0	23
GTACGAT	20	6.309312E-4	46.0	9
AGTACGG	35	1.0185977E-7	45.999996	1
>>END_MODULE