SRR1544970_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544970_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1378593
Sequences flagged as poor quality	0
Sequence length	52
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	4884	0.35427424918014233	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	3488	0.25301158500006893	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3280	0.23792373818813817	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2722	0.19744768760613174	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1686	0.12229860444670762	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1568	0.11373915288993923	No Hit
AGGCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAGA	1446	0.10488955043294142	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1444	0.1047444749828267	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTCG	20	6.311895E-4	46.0	9
TTCCCGA	25	3.4175137E-5	46.0	45
TCGTAGA	20	6.311895E-4	46.0	41
TCGCGAA	25	3.4175137E-5	46.0	15
ACGGTAC	20	6.311895E-4	46.0	12
ACGTAGG	165	0.0	44.60606	1
CATGCGG	280	0.0	44.357143	1
TAATCGT	170	0.0	43.294117	20
ATCGCGG	230	0.0	43.0	1
CGTCGGG	145	0.0	42.827583	2
ACGGGAT	430	0.0	42.7907	4
CTAGCGG	285	0.0	42.77193	1
AATACGG	120	0.0	42.166668	1
TACGGGA	295	0.0	42.101692	3
TTAATCG	175	0.0	42.057144	19
ACGATGG	115	0.0	42.0	1
AGTACGG	115	0.0	42.0	1
CGGTAGT	50	8.731149E-10	41.399998	11
CGTTTTT	2150	0.0	41.293022	1
GCATAGG	340	0.0	41.264706	1