##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544970_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1378593
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96101605042242	34.0	33.0	34.0	31.0	34.0
2	33.27436088823895	34.0	33.0	34.0	31.0	34.0
3	33.33586562531509	34.0	34.0	34.0	31.0	34.0
4	36.59872275573719	37.0	37.0	37.0	35.0	37.0
5	36.596452324942895	37.0	37.0	37.0	35.0	37.0
6	36.677981826398366	37.0	37.0	37.0	35.0	37.0
7	36.49389921463405	37.0	37.0	37.0	35.0	37.0
8	36.52685165237311	37.0	37.0	37.0	35.0	37.0
9	38.34124139611909	39.0	39.0	39.0	37.0	39.0
10	38.1851438386819	39.0	39.0	39.0	35.0	39.0
11	37.65426779332261	39.0	37.0	39.0	35.0	39.0
12	37.60108313331056	39.0	37.0	39.0	35.0	39.0
13	37.59315330920729	39.0	37.0	39.0	35.0	39.0
14	38.963552694667676	41.0	37.0	41.0	35.0	41.0
15	39.015548461366045	41.0	37.0	41.0	35.0	41.0
16	39.02107148375191	41.0	37.0	41.0	35.0	41.0
17	39.057472364940196	41.0	37.0	41.0	35.0	41.0
18	39.03793070180974	41.0	37.0	41.0	35.0	41.0
19	38.96589276167803	41.0	37.0	41.0	35.0	41.0
20	38.885911940652534	41.0	36.0	41.0	35.0	41.0
21	38.852515572036125	41.0	36.0	41.0	35.0	41.0
22	38.82056633103461	41.0	35.0	41.0	35.0	41.0
23	38.793291421035796	41.0	35.0	41.0	35.0	41.0
24	38.73153860493996	41.0	35.0	41.0	35.0	41.0
25	38.68925781575853	41.0	35.0	41.0	35.0	41.0
26	38.60877793518464	41.0	35.0	41.0	35.0	41.0
27	38.57863198202806	40.0	36.0	41.0	35.0	41.0
28	38.59755852525002	40.0	36.0	41.0	35.0	41.0
29	38.585702959466644	40.0	36.0	41.0	35.0	41.0
30	38.5393027528792	40.0	35.0	41.0	35.0	41.0
31	38.43189686876402	40.0	35.0	41.0	35.0	41.0
32	38.369925714115766	40.0	35.0	41.0	35.0	41.0
33	38.278071918252884	40.0	35.0	41.0	35.0	41.0
34	38.149294969581305	40.0	35.0	41.0	35.0	41.0
35	38.09495550898634	40.0	35.0	41.0	35.0	41.0
36	38.045381776927634	40.0	35.0	41.0	35.0	41.0
37	37.97019569952843	40.0	35.0	41.0	35.0	41.0
38	37.90049492489807	40.0	35.0	41.0	35.0	41.0
39	37.76942941100093	40.0	35.0	41.0	35.0	41.0
40	37.669206212420924	40.0	35.0	41.0	35.0	41.0
41	37.61930098295871	40.0	35.0	41.0	35.0	41.0
42	37.57258088500377	40.0	35.0	41.0	35.0	41.0
43	37.50288808952316	39.0	35.0	41.0	34.0	41.0
44	37.43419558927109	39.0	35.0	41.0	34.0	41.0
45	37.386163283869855	39.0	35.0	41.0	34.0	41.0
46	37.40356580948837	39.0	35.0	41.0	34.0	41.0
47	37.334065238979164	39.0	35.0	41.0	34.0	41.0
48	37.30323090281178	39.0	35.0	41.0	34.0	41.0
49	37.2439385663499	39.0	35.0	41.0	34.0	41.0
50	37.182386679752476	39.0	35.0	41.0	34.0	41.0
51	37.1030674027795	39.0	35.0	41.0	34.0	41.0
52	36.88222847497412	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	11.0
14	15.0
15	15.0
16	37.0
17	67.0
18	146.0
19	209.0
20	304.0
21	541.0
22	872.0
23	1392.0
24	2419.0
25	4052.0
26	5747.0
27	6965.0
28	7073.0
29	7526.0
30	8709.0
31	10783.0
32	13860.0
33	19281.0
34	58671.0
35	235284.0
36	53403.0
37	83269.0
38	146749.0
39	705188.0
40	5994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.988131377426114	21.999386330846015	23.69655148401305	20.31593080771482
2	28.252791070315897	24.25385882562874	25.361074660904272	22.132275443151098
3	28.400260265357506	24.721437001348477	24.539875075529906	22.33842765776411
4	26.653841996876526	26.890169905113403	21.69298698020373	24.763001117806343
5	24.246314902222775	36.74166342060347	20.013956258301036	18.99806541887272
6	87.45619628128098	3.146396362088013	6.748692326161529	2.6487150304694715
7	86.91716844637976	3.275078286339768	5.398910338294188	4.408842928986292
8	76.0679185227257	6.105935544428268	10.888928059260419	6.937217873585605
9	48.346248675279796	22.02724081726804	14.926885600028436	14.699624907423727
10	37.58447924804493	23.68168125037629	24.39530738949059	14.338532112088195
11	38.41293260592503	17.53454427811544	24.807394205541446	19.245128910418085
12	23.487135071772453	29.234806792142425	27.216226979246233	20.061831156838895
13	16.14994418222057	33.22583242479833	28.114824317256797	22.509399075724307
14	14.588569650360911	26.055043076528023	39.72898455164069	19.627402721470368
15	16.62898331849937	19.271242491438734	43.10800939798766	20.99176479207424
16	18.02134495097538	21.42466993521656	28.796606395070917	31.757378718737144
17	19.54833660115785	23.843005150903856	30.734306644528154	25.874351603410144
18	24.22223237750373	27.652904084091535	26.90322669562373	21.22163684278101
19	28.50507727806539	22.33646913918756	25.529289645312286	23.62916393743476
20	23.217802498634477	27.912153913446534	26.480041607639095	22.390001980279894
21	20.967101965554736	24.952469655656166	25.078032457730455	29.002395921058643
22	20.57728423109649	28.72602718859011	23.802964326672193	26.89372425364121
23	20.715831285956043	22.488072984557444	34.0106180721939	22.785477657292617
24	20.456001154800582	22.325443404978845	31.559060578430326	25.659494861790243
25	19.138063228233424	29.402296399299864	27.791233525775915	23.668406846690793
26	20.09650418941631	29.398887126222167	26.62787349130599	23.876735193055527
27	22.052701558763175	27.124974521124074	31.562179700607796	19.26014421950496
28	19.321801285803712	26.09414091033394	29.825699100459673	24.75835870340267
29	21.352712511959655	27.63491472827731	27.470471705572276	23.54190105419076
30	26.75314614248005	24.801953876162145	23.827264464566408	24.617635516791395
31	26.954293254064112	26.66653609876156	25.811606471235528	20.567564175938802
32	27.141658197887264	26.785643043305747	25.397125910257778	20.67557284854921
33	23.733183035167013	25.750892395362516	26.71629697815091	23.799627591319556
34	22.581791725331552	22.522310790784516	31.59830348768636	23.29759399619757
35	29.00420936418508	22.91945483547356	26.28288407093319	21.793451729408172
36	25.91475511626709	26.553812474022426	27.706436925183866	19.824995484526614
37	25.452907420826886	25.279107031589454	28.528071736908572	20.739913810675088
38	24.692566986775645	25.247190432564214	26.29964028542144	23.760602295238698
39	29.573267817260064	21.901968166093983	27.92223665722951	20.60252735941645
40	20.603978113917595	21.332184335768424	31.530988478833127	26.53284907148085
41	23.245294296431215	25.485259246202467	25.33285748585696	25.936588971509355
42	24.62546959109759	22.98647969342656	27.957707604782556	24.430343110693293
43	23.939552862955203	26.90554790282556	27.99593498588779	21.15896424833145
44	21.012510581440644	27.702592425755824	27.301023579838287	23.98387341296525
45	22.700826132150677	26.441379000183517	26.51863167736961	24.339163190296194
46	24.716504436044577	23.714976066177616	28.294065035873533	23.274454461904277
47	24.533056529374512	20.37367083686048	31.462222715478756	23.631049918286255
48	22.64446431978111	20.95462547684487	28.88553764599124	27.515372557382783
49	21.313034376353283	24.65404945477019	30.786823957469682	23.246092211406847
50	21.019256589870977	25.103275586050415	27.927604448883752	25.949863375194855
51	21.8701241047938	22.78460720459193	29.931386565868245	25.413882124746028
52	24.1923468347801	19.863658091982188	32.104036506786265	23.839958566451447
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	130.0
1	430.5
2	731.0
3	2949.5
4	5168.0
5	3538.5
6	1909.0
7	2046.5
8	2184.0
9	2194.0
10	2204.0
11	2086.5
12	1969.0
13	1914.5
14	1751.5
15	1643.0
16	1606.5
17	1570.0
18	1791.0
19	2012.0
20	2328.5
21	2645.0
22	2944.5
23	3244.0
24	3691.5
25	4139.0
26	5535.0
27	6931.0
28	9842.5
29	12754.0
30	13828.0
31	14902.0
32	15355.0
33	15808.0
34	20001.5
35	24195.0
36	26320.5
37	28446.0
38	31550.0
39	40900.0
40	47146.0
41	57679.5
42	68213.0
43	85584.5
44	102956.0
45	114660.0
46	126364.0
47	133633.5
48	140903.0
49	140273.5
50	139644.0
51	126155.0
52	112666.0
53	99835.5
54	87005.0
55	75535.0
56	64065.0
57	58589.0
58	53113.0
59	54754.0
60	56395.0
61	60183.0
62	63971.0
63	57778.5
64	42404.0
65	33222.0
66	27512.5
67	21803.0
68	17811.5
69	13820.0
70	11387.5
71	8955.0
72	8189.5
73	7424.0
74	6240.0
75	5056.0
76	3886.5
77	2717.0
78	2108.0
79	1499.0
80	1022.5
81	546.0
82	399.0
83	252.0
84	161.5
85	71.0
86	73.0
87	75.0
88	46.5
89	12.5
90	7.0
91	4.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1378593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.273970531790926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73088030282848	14.834526927182134
2	12.134691861081665	5.163061541300291
3	4.693177030270583	2.9952752952736326
4	2.2767119176292887	1.937388089800908
5	1.362198507035348	1.4489685448559793
6	0.842768480534884	1.0757419092012803
7	0.5612566426598862	0.8358110093702829
8	0.41773151316973145	0.7109446321098641
9	0.37209598749184736	0.7124363165609285
>10	5.284144974547568	29.95389276961575
>50	2.070448974819521	28.802113867212174
>100	0.23920008822593805	8.038602975514749
>500	0.008884574705522584	1.2298020088684853
>1k	0.005809144999764767	2.2614341131335842
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	4884	0.35427424918014233	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	3488	0.25301158500006893	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3280	0.23792373818813817	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2722	0.19744768760613174	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1686	0.12229860444670762	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1568	0.11373915288993923	No Hit
AGGCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAGA	1446	0.10488955043294142	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1444	0.1047444749828267	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.258887140729715	0.0
2	0.0	0.0	0.0	1.0212586310825602	0.0
3	0.0	0.0	0.0	1.6085240531469405	0.0
4	0.0	0.0	0.0	1.9700520748328187	0.0
5	0.0	0.0	0.0	4.052900312129831	0.0
6	0.0	0.0	0.0	4.427485124326034	0.0
7	0.0	0.0	0.0	6.16164451727232	0.0
8	0.0	0.0	0.0	7.999460319325573	0.0
9	0.0	0.0	0.0	9.533488128838606	0.0
10	0.0	0.0	0.0	10.38957835996556	0.0
11	0.0	0.0	0.0	11.209327190838776	0.0
12	0.0	0.0	0.0	11.928466197057435	0.0
13	7.25377250573592E-5	0.0	0.0	12.225725794342493	0.0
14	7.25377250573592E-5	0.0	0.0	12.437535951509982	0.0
15	7.25377250573592E-5	0.0	0.0	13.20614568621776	0.0
16	7.25377250573592E-5	0.0	0.0	14.029739016519017	0.0
17	7.25377250573592E-5	0.0	0.0	15.122882533133419	0.0
18	7.25377250573592E-5	0.0	0.0	15.626149269581378	0.0
19	7.25377250573592E-5	0.0	0.0	16.232709726511015	0.0
20	7.25377250573592E-5	0.0	0.0	16.924574548108108	0.0
21	7.25377250573592E-5	0.0	0.0	17.56479250946436	0.0
22	7.25377250573592E-5	0.0	0.0	18.292273354064616	0.0
23	7.25377250573592E-5	0.0	0.0	18.79597531686292	0.0
24	7.25377250573592E-5	0.0	0.0	19.24505637269303	0.0
25	7.25377250573592E-5	0.0	0.0	19.677816440385232	0.0
26	7.25377250573592E-5	0.0	0.0	20.096068963065967	0.0
27	7.25377250573592E-5	0.0	0.0	20.51156505219452	0.0
28	7.25377250573592E-5	0.0	0.0	20.912698671761717	0.0
29	1.450754501147184E-4	0.0	0.0	21.420897973513576	0.0
30	1.450754501147184E-4	0.0	0.0	21.92119066323418	0.0
31	1.450754501147184E-4	0.0	0.0	22.40276861988999	0.0
32	1.450754501147184E-4	0.0	0.0	22.825518481524277	0.0
33	1.450754501147184E-4	0.0	0.0	23.229698685543884	0.0
34	1.450754501147184E-4	0.0	0.0	23.756322569460313	0.0
35	1.450754501147184E-4	0.0	0.0	24.19640894738331	0.0
36	1.450754501147184E-4	0.0	0.0	24.70867036173838	0.0
37	1.450754501147184E-4	0.0	0.0	25.12431152631705	0.0
38	1.450754501147184E-4	0.0	0.0	25.52754873991091	0.0
39	1.450754501147184E-4	0.0	0.0	25.957552374050934	0.0
40	1.450754501147184E-4	0.0	0.0	26.420270522191828	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTCG	20	6.311895E-4	46.0	9
TTCCCGA	25	3.4175137E-5	46.0	45
TCGTAGA	20	6.311895E-4	46.0	41
TCGCGAA	25	3.4175137E-5	46.0	15
ACGGTAC	20	6.311895E-4	46.0	12
ACGTAGG	165	0.0	44.60606	1
CATGCGG	280	0.0	44.357143	1
TAATCGT	170	0.0	43.294117	20
ATCGCGG	230	0.0	43.0	1
CGTCGGG	145	0.0	42.827583	2
ACGGGAT	430	0.0	42.7907	4
CTAGCGG	285	0.0	42.77193	1
AATACGG	120	0.0	42.166668	1
TACGGGA	295	0.0	42.101692	3
TTAATCG	175	0.0	42.057144	19
ACGATGG	115	0.0	42.0	1
AGTACGG	115	0.0	42.0	1
CGGTAGT	50	8.731149E-10	41.399998	11
CGTTTTT	2150	0.0	41.293022	1
GCATAGG	340	0.0	41.264706	1
>>END_MODULE