FastQCFastQC Report
Thu 26 May 2016
SRR1544969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544969_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences854292
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT35760.4185922377828658Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT26750.3131247863728093Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA25850.30258974683129425No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT25810.3021215228516713Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.21292485473351033No Hit
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTTG15920.18635314388991117Illumina Single End Adapter 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT9650.11295903508402279Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9520.1114373071502484No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9120.10675506735401948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG551.8189894E-1246.0000041
CGTGGTA206.310541E-446.03
CGTCTAT206.310541E-446.020
CGATTCA253.4164135E-546.09
TCGTCTA206.310541E-446.019
CGGTATT301.8608389E-646.043
CGTCCGA206.310541E-446.038
AATACGG405.6079443E-946.01
ATACCGG206.310541E-446.01
GTAACGG1000.046.01
GCGTACT206.310541E-446.025
TCACGTC206.310541E-446.038
AACACGA206.310541E-446.01
ACGGGAT2250.043.9555554
GCTACGA950.043.57894510
TCGTTCA900.043.44444315
CGAATAT850.043.29411714
CTAGCGG2400.043.1251
ACGTAGG750.042.933331
AACACGT750.042.9333340
Produced by FastQC (version 0.11.5)