##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544969_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	854292
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94180912381247	34.0	33.0	34.0	31.0	34.0
2	33.296823568522235	34.0	34.0	34.0	31.0	34.0
3	33.42570104835349	34.0	34.0	34.0	31.0	34.0
4	36.62892781390906	37.0	37.0	37.0	35.0	37.0
5	36.6375138711354	37.0	37.0	37.0	35.0	37.0
6	36.43074967341377	37.0	37.0	37.0	35.0	37.0
7	36.23408623749257	37.0	37.0	37.0	35.0	37.0
8	36.37350578022503	37.0	37.0	37.0	35.0	37.0
9	38.27297809179999	39.0	39.0	39.0	37.0	39.0
10	38.20077678358219	39.0	39.0	39.0	35.0	39.0
11	37.7187706311191	39.0	37.0	39.0	35.0	39.0
12	37.71651379153732	39.0	37.0	39.0	35.0	39.0
13	37.71682281936387	39.0	37.0	39.0	35.0	39.0
14	39.019029793091825	41.0	38.0	41.0	35.0	41.0
15	39.047337444339874	41.0	38.0	41.0	35.0	41.0
16	39.07857032490062	41.0	38.0	41.0	35.0	41.0
17	39.14414275212691	41.0	38.0	41.0	35.0	41.0
18	39.1391866013026	41.0	38.0	41.0	35.0	41.0
19	39.095854813108396	41.0	37.0	41.0	35.0	41.0
20	39.032136552841415	41.0	37.0	41.0	35.0	41.0
21	38.98979271724422	41.0	37.0	41.0	35.0	41.0
22	38.969333670454596	41.0	37.0	41.0	35.0	41.0
23	38.924974130625124	41.0	36.0	41.0	35.0	41.0
24	38.87075379378479	41.0	36.0	41.0	35.0	41.0
25	38.82477419898583	41.0	36.0	41.0	35.0	41.0
26	38.72426289840008	41.0	36.0	41.0	35.0	41.0
27	38.79915415338081	41.0	36.0	41.0	35.0	41.0
28	38.79686219700056	41.0	36.0	41.0	35.0	41.0
29	38.79366305665978	41.0	36.0	41.0	35.0	41.0
30	38.75556367143787	41.0	36.0	41.0	35.0	41.0
31	38.64015699550037	41.0	35.0	41.0	35.0	41.0
32	38.60383217916122	40.0	36.0	41.0	35.0	41.0
33	38.51364872900601	40.0	36.0	41.0	35.0	41.0
34	38.36600483207147	40.0	35.0	41.0	35.0	41.0
35	38.318432105181834	40.0	35.0	41.0	35.0	41.0
36	38.29592223736147	40.0	35.0	41.0	35.0	41.0
37	38.224590655185814	40.0	35.0	41.0	35.0	41.0
38	38.162014861429114	40.0	35.0	41.0	35.0	41.0
39	38.09854827155118	40.0	35.0	41.0	35.0	41.0
40	38.03320527407491	40.0	35.0	41.0	35.0	41.0
41	37.98014847382394	40.0	35.0	41.0	35.0	41.0
42	37.94058237698585	40.0	35.0	41.0	35.0	41.0
43	37.884878940690065	40.0	35.0	41.0	35.0	41.0
44	37.689270179282964	40.0	35.0	41.0	35.0	41.0
45	37.70446521798167	40.0	35.0	41.0	35.0	41.0
46	37.66574543598676	40.0	35.0	41.0	35.0	41.0
47	37.58683213702106	39.0	35.0	41.0	35.0	41.0
48	37.51141647118315	39.0	35.0	41.0	35.0	41.0
49	37.46297401825137	39.0	35.0	41.0	35.0	41.0
50	37.4078699086495	39.0	35.0	41.0	35.0	41.0
51	37.32644575859308	39.0	35.0	41.0	35.0	41.0
52	36.837008891573376	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	5.0
14	5.0
15	5.0
16	16.0
17	44.0
18	60.0
19	123.0
20	193.0
21	298.0
22	493.0
23	739.0
24	1141.0
25	2164.0
26	3170.0
27	3746.0
28	3678.0
29	3697.0
30	4204.0
31	5118.0
32	6788.0
33	10045.0
34	34169.0
35	139545.0
36	31366.0
37	51389.0
38	93978.0
39	456063.0
40	2044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.96953266564594	21.75216436534581	23.10954568227257	21.16875728673568
2	28.35786826986557	24.182246819588617	25.12993215434535	22.329952756200456
3	28.49236560801225	24.565019922930333	24.467395223178958	22.47521924587846
4	26.632111736970497	26.314187654806553	22.072429567407863	24.981271040815084
5	24.07572586422441	36.19745941668657	20.221423119963667	19.505391599125357
6	86.08110575775028	3.1252780079879012	7.469460090929096	3.3241561433327247
7	85.67070743961082	3.1722174619450962	6.019019258052282	5.13805584039181
8	74.07279946435177	6.138065204871402	11.966634359212073	7.822500971564758
9	47.57518506552795	21.43786901902394	15.462511647071493	15.524434268376622
10	36.53188839413222	23.10076648265464	25.762268638826068	14.605076484387073
11	36.89604959428392	17.488985030879373	25.267238836369764	20.34772653846694
12	22.910082266953218	28.212484724192667	28.27955780927364	20.59787519958047
13	16.1539614089796	32.017623950593006	28.816727769896005	23.011686870531385
14	14.520093832085518	25.649894883716573	39.162487767648535	20.667523516549377
15	16.359160568049333	19.071347970014937	42.75200985143253	21.8174816105032
16	17.770621754622542	21.963450436150637	29.089000014046718	31.176927795180102
17	19.174240189537066	23.850393074030894	30.9601400926147	26.015226643817336
18	23.534575999775253	27.82959456485605	27.156054370168516	21.479775065200187
19	27.471754388429247	22.376306930183123	25.802887069058357	24.349051612329273
20	22.436941935544287	28.665374368482908	26.424337346012837	22.473346349959968
21	20.55713971335328	24.70080487702097	25.25014866111353	29.49190674851222
22	20.20538644866158	28.84025602487206	23.913252143295267	27.041105383171093
23	20.163363346490428	22.55891428223605	34.12498302688074	23.152739344392785
24	20.51933062699873	22.76551811324465	31.035875321318706	25.67927593843791
25	19.216614459692938	29.015605905240832	27.80243757403792	23.965342061028313
26	19.959920027344282	28.680240479835934	26.68595749462713	24.673881998192655
27	22.750886113881435	26.2967463115656	31.420638376573816	19.531729197979146
28	19.693500582938857	25.734877536018132	29.50489996394675	25.066721917096263
29	21.541931798495128	27.465901588683963	27.36827688893259	23.62388972388832
30	27.306822491607086	24.447027480065366	23.3698782149429	24.87627181338465
31	27.031155623604107	26.128536846886075	25.73288758410473	21.107419945405084
32	26.902276973212906	26.234823690260473	25.75454294316229	21.108356393364332
33	24.103819303001785	25.222874614300494	26.86704311874628	23.806262963951436
34	23.199444686360167	21.737181197997874	32.2956319384941	22.76774217714786
35	29.317727428092503	22.254217527496454	26.86716017474119	21.560894869669855
36	26.606008250106523	25.768823774540788	27.65623463640067	19.96893333895202
37	26.083353232852467	24.657494158905855	28.923599893244933	20.335552714996748
38	25.108510907277605	24.55600661132259	26.200760395742904	24.134722085656897
39	30.328623000098325	21.29201724937141	27.776217031179034	20.60314271935123
40	21.234308643882887	20.53688902623459	31.514049060508587	26.714753269373936
41	23.43168378025312	25.1902159917218	25.41297355002739	25.965126677997684
42	24.43696066450347	22.285822646120998	28.33632996680292	24.94088672257261
43	24.749500170901754	26.07773454509699	27.811684997635467	21.361080286365784
44	21.18713507793588	26.835320944126835	27.82315648513623	24.154387492801057
45	22.878711260318486	25.55613303179709	26.722478965037716	24.84267674284671
46	25.12162117870705	22.5967233685906	28.517532646916976	23.764122805785377
47	24.36941935544287	19.725690981537927	32.00264078324507	23.90224887977413
48	22.63781002280251	20.02043797671054	28.882630295027926	28.459121705459022
49	21.447350554611305	23.388138950148193	31.34502020386472	23.819490291375782
50	21.193104933676075	23.906111727606017	28.662447968610266	26.238335370107645
51	22.033566977099163	21.736361806033532	30.074611491152908	26.15545972571439
52	24.195942370992586	19.29703192819317	32.64527819527749	23.861747505536748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	75.0
1	271.0
2	467.0
3	1891.0
4	3315.0
5	2265.5
6	1216.0
7	1268.0
8	1320.0
9	1356.0
10	1392.0
11	1353.5
12	1315.0
13	1237.5
14	1109.0
15	1058.0
16	1043.0
17	1028.0
18	1147.0
19	1266.0
20	1372.5
21	1479.0
22	1642.5
23	1806.0
24	2068.0
25	2330.0
26	3052.5
27	3775.0
28	5320.0
29	6865.0
30	7484.5
31	8104.0
32	8512.5
33	8921.0
34	11303.0
35	13685.0
36	14974.5
37	16264.0
38	18058.0
39	23201.5
40	26551.0
41	32899.5
42	39248.0
43	49766.5
44	60285.0
45	68525.0
46	76765.0
47	84324.0
48	91883.0
49	92602.5
50	93322.0
51	84164.5
52	75007.0
53	65793.0
54	56579.0
55	48529.5
56	40480.0
57	36703.5
58	32927.0
59	33745.5
60	34564.0
61	36319.5
62	38075.0
63	35398.5
64	26496.5
65	20271.0
66	16927.0
67	13583.0
68	11188.0
69	8793.0
70	7170.5
71	5548.0
72	5091.5
73	4635.0
74	3913.0
75	3191.0
76	2421.0
77	1651.0
78	1293.0
79	935.0
80	637.0
81	339.0
82	249.0
83	159.0
84	106.5
85	54.0
86	42.0
87	30.0
88	15.5
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	854292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.832923165567994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9789490257405	18.335937365547217
2	11.257102108402231	5.816077076666166
3	4.06877340302798	3.1532493209558523
4	1.9338601758334364	1.998290453410279
5	1.1233029091198425	1.450909887147595
6	0.7422633013475932	1.1504898499399931
7	0.5710126941367373	1.0325648937938816
8	0.4998601947684529	1.0330280003983436
9	0.42002950579552234	0.9765530955438503
>10	7.859669689556228	49.83130029664685
>50	0.4359595647920066	7.401721983759104
>100	0.09829568473149043	4.562094993319775
>500	0.008191307060957536	1.5146244722326379
>1k	0.002730435686985845	1.7431583106384314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3576	0.4185922377828658	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	2675	0.3131247863728093	Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2585	0.30258974683129425	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	2581	0.3021215228516713	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1819	0.21292485473351033	No Hit
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTTG	1592	0.18635314388991117	Illumina Single End Adapter 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	965	0.11295903508402279	Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	952	0.1114373071502484	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	912	0.10675506735401948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31967992208752977	0.0
2	0.0	0.0	0.0	1.2472316257204796	0.0
3	0.0	0.0	0.0	1.9423101234706635	0.0
4	0.0	0.0	0.0	2.3963703277099633	0.0
5	0.0	0.0	0.0	4.908274922391875	0.0
6	0.0	0.0	0.0	5.356131158901172	0.0
7	0.0	0.0	0.0	7.4058986856952895	0.0
8	0.0	0.0	0.0	9.560782495914745	0.0
9	0.0	0.0	0.0	11.459079565300858	0.0
10	0.0	0.0	0.0	12.494205728252167	0.0
11	0.0	0.0	0.0	13.451957878570793	0.0
12	0.0	0.0	0.0	14.297687441764642	0.0
13	0.0	0.0	0.0	14.648387202502189	0.0
14	0.0	0.0	0.0	14.897833527646284	0.0
15	0.0	0.0	0.0	15.790970768776953	0.0
16	0.0	0.0	0.0	16.68843908171913	0.0
17	0.0	0.0	0.0	17.83383199187163	0.0
18	0.0	0.0	0.0	18.388911519714572	0.0
19	0.0	0.0	0.0	19.028154307894724	0.0
20	0.0	0.0	0.0	19.780004963174186	0.0
21	0.0	0.0	0.0	20.49392947610419	0.0
22	0.0	0.0	0.0	21.295177761233862	0.0
23	0.0	0.0	0.0	21.838668745581135	0.0
24	0.0	0.0	0.0	22.31614014880158	0.0
25	0.0	0.0	0.0	22.77289264092371	0.0
26	0.0	0.0	0.0	23.20482926212583	0.0
27	0.0	0.0	0.0	23.645076858966256	0.0
28	0.0	0.0	0.0	24.073618856316106	0.0
29	0.0	0.0	0.0	24.58702644997261	0.0
30	0.0	0.0	0.0	25.125718138528747	0.0
31	0.0	0.0	0.0	25.61501219723467	0.0
32	0.0	0.0	0.0	26.03758433884433	0.0
33	0.0	0.0	0.0	26.457230080581347	0.0
34	0.0	0.0	0.0	26.988196073473706	0.0
35	0.0	0.0	0.0	27.42493199046696	0.0
36	0.0	0.0	0.0	27.912119041264578	0.0
37	0.0	0.0	0.0	28.330711279047446	0.0
38	0.0	0.0	0.0	28.747079452927103	0.0
39	0.0	0.0	0.0	29.169066314562233	0.0
40	0.0	0.0	0.0	29.629915766506066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGG	55	1.8189894E-12	46.000004	1
CGTGGTA	20	6.310541E-4	46.0	3
CGTCTAT	20	6.310541E-4	46.0	20
CGATTCA	25	3.4164135E-5	46.0	9
TCGTCTA	20	6.310541E-4	46.0	19
CGGTATT	30	1.8608389E-6	46.0	43
CGTCCGA	20	6.310541E-4	46.0	38
AATACGG	40	5.6079443E-9	46.0	1
ATACCGG	20	6.310541E-4	46.0	1
GTAACGG	100	0.0	46.0	1
GCGTACT	20	6.310541E-4	46.0	25
TCACGTC	20	6.310541E-4	46.0	38
AACACGA	20	6.310541E-4	46.0	1
ACGGGAT	225	0.0	43.955555	4
GCTACGA	95	0.0	43.578945	10
TCGTTCA	90	0.0	43.444443	15
CGAATAT	85	0.0	43.294117	14
CTAGCGG	240	0.0	43.125	1
ACGTAGG	75	0.0	42.93333	1
AACACGT	75	0.0	42.93333	40
>>END_MODULE