FastQCFastQC Report
Thu 26 May 2016
SRR1544968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544968_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1104400
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT41970.38002535313292285Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT31760.28757696486780154Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT29890.27064469395146684Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21680.19630568634552698No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16970.15365809489315466No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT12560.11372691053965955No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA11790.10675479898587469No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11630.10530604853314016No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT11170.10114089098152844Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC11120.10068815646504889No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG206.311347E-446.01
CACGACG301.8613355E-646.025
AATATCG206.311347E-446.028
TGTTCGG206.311347E-446.01
ACTACGC206.311347E-446.010
ATCCACG253.417069E-546.034
GCGATAT405.6115823E-946.08
CGTACGA301.8613355E-646.016
CTACCGA206.311347E-446.021
ACTCGAA206.311347E-446.040
CGTAAGG253.417069E-546.01
TGGGCGA2200.046.05
TAAGACG206.311347E-446.025
TAATGCG253.417069E-546.01
CGATACG253.417069E-546.09
AATACGG600.046.01
TCGAATA206.311347E-446.032
AGTACGT206.311347E-446.016
CGCCCGA206.311347E-446.034
GTACGTC206.311347E-446.017
Produced by FastQC (version 0.11.5)