##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544968_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1104400
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91770644693951	34.0	33.0	34.0	31.0	34.0
2	33.22954636001449	34.0	33.0	34.0	31.0	34.0
3	33.28519195943499	34.0	34.0	34.0	31.0	34.0
4	36.5608955088736	37.0	37.0	37.0	35.0	37.0
5	36.554836110105036	37.0	37.0	37.0	35.0	37.0
6	36.65393154654111	37.0	37.0	37.0	35.0	37.0
7	36.471784679463966	37.0	37.0	37.0	35.0	37.0
8	36.50997011952191	37.0	37.0	37.0	35.0	37.0
9	38.316128214415066	39.0	39.0	39.0	37.0	39.0
10	38.14549348062296	39.0	39.0	39.0	35.0	39.0
11	37.5174148859109	39.0	35.0	39.0	35.0	39.0
12	37.43802426657008	39.0	35.0	39.0	35.0	39.0
13	37.42043190872872	39.0	35.0	39.0	35.0	39.0
14	38.751280333212605	41.0	35.0	41.0	35.0	41.0
15	38.8170988772184	41.0	35.0	41.0	35.0	41.0
16	38.82634643245201	41.0	35.0	41.0	35.0	41.0
17	38.8695961608113	41.0	36.0	41.0	35.0	41.0
18	38.85528884462151	41.0	36.0	41.0	35.0	41.0
19	38.78004346251358	41.0	36.0	41.0	35.0	41.0
20	38.69356120970663	41.0	35.0	41.0	35.0	41.0
21	38.63520282506338	41.0	35.0	41.0	35.0	41.0
22	38.60518109380659	41.0	35.0	41.0	35.0	41.0
23	38.58652118797537	41.0	35.0	41.0	35.0	41.0
24	38.519640528793914	40.0	35.0	41.0	35.0	41.0
25	38.48836381745744	40.0	35.0	41.0	35.0	41.0
26	38.43139894965592	40.0	35.0	41.0	35.0	41.0
27	38.39195219123506	40.0	35.0	41.0	35.0	41.0
28	38.42773994929373	40.0	36.0	41.0	35.0	41.0
29	38.436136363636365	40.0	36.0	41.0	35.0	41.0
30	38.33172039116262	40.0	35.0	41.0	35.0	41.0
31	38.28110376675118	40.0	35.0	41.0	35.0	41.0
32	38.176571894241214	40.0	35.0	41.0	35.0	41.0
33	38.07910630206447	40.0	35.0	41.0	35.0	41.0
34	37.952658457080766	40.0	35.0	41.0	35.0	41.0
35	37.9054092720029	40.0	35.0	41.0	35.0	41.0
36	37.8761427019196	40.0	35.0	41.0	35.0	41.0
37	37.80090184715683	40.0	35.0	41.0	35.0	41.0
38	37.75734425932633	40.0	35.0	41.0	35.0	41.0
39	37.63055686345527	40.0	35.0	41.0	35.0	41.0
40	37.511818181818185	40.0	35.0	41.0	34.0	41.0
41	37.45440239043825	39.0	35.0	41.0	34.0	41.0
42	37.35854491126403	39.0	35.0	41.0	34.0	41.0
43	37.275083303151035	39.0	35.0	41.0	34.0	41.0
44	37.20423940601231	39.0	35.0	41.0	34.0	41.0
45	37.16744929373415	39.0	35.0	41.0	34.0	41.0
46	37.18336472292648	39.0	35.0	41.0	34.0	41.0
47	37.09940148496921	39.0	35.0	41.0	34.0	41.0
48	37.09124683085838	39.0	35.0	41.0	34.0	41.0
49	37.0054862368707	38.0	35.0	41.0	34.0	41.0
50	36.881034951104674	38.0	35.0	41.0	34.0	41.0
51	36.699897681999275	37.0	35.0	40.0	34.0	41.0
52	36.42229536399855	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	11.0
15	13.0
16	33.0
17	63.0
18	91.0
19	207.0
20	302.0
21	482.0
22	782.0
23	1299.0
24	2128.0
25	3544.0
26	5093.0
27	6150.0
28	6309.0
29	6858.0
30	7707.0
31	9538.0
32	12328.0
33	17656.0
34	51759.0
35	206529.0
36	40973.0
37	64970.0
38	124143.0
39	531695.0
40	3730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.09199565374864	21.517294458529516	23.608022455632018	20.782687432089823
2	28.10956175298805	23.812386816370882	25.795273451647954	22.282777978993117
3	27.527164070988775	24.160720753350233	25.364451285766027	22.947663889894965
4	26.020282506338283	27.531600869250273	22.427381383556682	24.020735240854762
5	23.82343353857298	36.42747193045997	20.572980804056503	19.17611372691054
6	86.98487866714957	3.2172220210068816	7.088826512133285	2.70907279971025
7	86.53558493299529	3.1556501267656647	5.559398768562115	4.749366171676929
8	76.14252082578776	5.181546541108294	11.374411445128576	7.301521187975371
9	49.1504889532778	20.524538210793192	15.135095979717494	15.189876856211518
10	38.971930459978275	21.317185802245564	24.641253169141617	15.069630568634555
11	36.93571169865991	17.415338645418327	25.992756247736327	19.65619340818544
12	20.309942049981892	31.81302064469395	28.87097066280333	19.006066642520825
13	15.02544367982615	34.86146323795726	29.49936617167693	20.613726910539658
14	13.424483882651215	22.824610648315826	43.580134009416874	20.17077145961608
15	15.614632379572619	17.74157913799348	45.1526620789569	21.491126403477
16	16.54554509235784	20.500362187613185	27.79518290474466	35.15890981528432
17	18.702915610286126	22.93073161897863	33.6594530967041	24.70689967403115
18	24.047356030423757	25.766026801883374	28.165700833031508	22.020916334661354
19	26.17185802245563	21.77788844621514	26.469485693589277	25.58076783773995
20	20.96287576964868	28.77969938428106	27.27254617892068	22.984878667149584
21	21.334842448388265	23.66796450561391	25.880206446939514	29.116986599058315
22	18.637088011590002	29.25968851865266	24.03630930822166	28.066914161535678
23	19.12504527345165	21.881926838102135	36.3164614270192	22.67656646142702
24	19.810032596885186	22.049257515392974	33.19422310756972	24.946486780152117
25	17.90664614270192	29.105758783049616	28.82678377399493	24.16081130025353
26	19.75262586019558	28.228178196305688	27.279518290474464	24.739677653024266
27	19.89351684172401	25.98560304237595	34.80450923578414	19.316370880115898
28	17.27716407098877	24.800796812749006	31.209163346613543	26.71287576964868
29	20.96224194132561	26.473741398044186	28.513491488591093	24.050525172039116
30	25.768471568272368	23.938337558855487	25.703458891705903	24.589731981166242
31	28.28956899674031	26.046722202100685	24.20155740673669	21.46215139442231
32	26.244929373415427	25.008149221296634	24.400760593987687	24.346160811300255
33	21.138808402752627	25.01657008330315	28.60974284679464	25.234878667149584
34	19.997736327417602	23.857207533502354	28.6681455994205	27.476910539659542
35	28.489406012314376	22.68181818181818	25.693136544730173	23.13563926113727
36	23.23605577689243	25.425479898587465	27.632470119521912	23.70599420499819
37	21.543009779065557	26.55052517203912	27.673578413618255	24.232886635277072
38	22.830586743933356	25.784136182542554	24.923850054328142	26.461427019195945
39	28.726367258239765	21.096704092720028	26.783502354219486	23.393426294820717
40	19.364722926475913	20.359833393697933	30.643335747917423	29.63210793190873
41	23.822256428830137	24.26059398768562	24.21930459978269	27.697844983701557
42	23.348333936979355	22.427471930459976	28.040746106483162	26.18344802607751
43	23.11101050344078	26.283411807316188	27.849148859109018	22.75642883013401
44	18.790112278160088	28.540836653386453	26.65085114089098	26.01819992756248
45	22.989496559217677	25.1527526258602	27.232252806954005	24.625498007968126
46	24.63654473017023	22.07678377399493	27.5641072075335	25.722564288301342
47	24.031329228540386	20.130478087649404	30.541923216226003	25.29626946758421
48	21.41235059760956	20.185892792466497	28.811662441144513	29.59009416877943
49	21.51303875407461	23.71522998913437	30.14532777978993	24.626403477001084
50	20.643697935530607	24.095255342267293	28.214052879391527	27.046993842810572
51	20.951014125316913	20.791923216226003	30.18163708801159	28.07542557044549
52	23.446758420862007	19.162260050706266	31.855306048533137	25.53567547989859
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	355.5
2	543.0
3	2350.5
4	4158.0
5	2844.5
6	1531.0
7	1677.0
8	1823.0
9	1856.5
10	1890.0
11	1805.0
12	1720.0
13	1664.0
14	1618.5
15	1629.0
16	1575.5
17	1522.0
18	1550.0
19	1578.0
20	1862.5
21	2147.0
22	2360.0
23	2573.0
24	2844.5
25	3116.0
26	3183.0
27	3250.0
28	5102.5
29	6955.0
30	7871.5
31	8788.0
32	10256.0
33	11724.0
34	14219.0
35	16714.0
36	18666.0
37	20618.0
38	22487.5
39	28413.5
40	32470.0
41	41405.0
42	50340.0
43	64134.0
44	77928.0
45	86959.5
46	95991.0
47	96744.5
48	97498.0
49	106966.0
50	116434.0
51	120623.5
52	124813.0
53	110683.0
54	96553.0
55	81932.0
56	67311.0
57	58587.0
58	49863.0
59	46864.0
60	43865.0
61	41614.5
62	39364.0
63	35030.0
64	26621.5
65	22547.0
66	18017.0
67	13487.0
68	11539.0
69	9591.0
70	8087.5
71	6584.0
72	5306.5
73	4029.0
74	3594.0
75	3159.0
76	2551.0
77	1943.0
78	1293.5
79	644.0
80	587.0
81	530.0
82	362.5
83	195.0
84	158.5
85	122.0
86	71.0
87	20.0
88	14.5
89	5.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1104400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.400416994503903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.23959278310906	15.50995750871286
2	12.632641006100837	5.65952852557056
3	4.97419186438076	3.3427191591839343
4	2.4503975758805288	2.195597100081814
5	1.378837947165733	1.5443272492179079
6	0.8623683390675189	1.1590446238782093
7	0.5956236535669772	0.9339552748183158
8	0.4378002407524375	0.7845526362519041
9	0.3536470609773508	0.7129657471296067
>10	4.917729408887546	29.92099717595457
>50	1.934209938875377	28.02955775229405
>100	0.21284402056275514	7.3690529068779025
>500	0.0056650499769554585	0.9205650978160834
>1k	0.004451110696179288	1.9171792422122864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	4197	0.38002535313292285	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT	3176	0.28757696486780154	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	2989	0.27064469395146684	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2168	0.19630568634552698	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1697	0.15365809489315466	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1256	0.11372691053965955	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1179	0.10675479898587469	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1163	0.10530604853314016	No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	1117	0.10114089098152844	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	1112	0.10068815646504889	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29690329590728	0.0
2	0.0	0.0	0.0	1.1315646504889534	0.0
3	0.0	0.0	0.0	1.781329228540384	0.0
4	0.0	0.0	0.0	2.1867982614994568	0.0
5	0.0	0.0	0.0	4.43761318362912	0.0
6	0.0	0.0	0.0	4.851412531691416	0.0
7	0.0	0.0	0.0	6.8839188699746465	0.0
8	0.0	0.0	0.0	9.076602680188337	0.0
9	0.0	0.0	0.0	11.132198478812025	0.0
10	0.0	0.0	0.0	12.069902209344441	0.0
11	0.0	0.0	0.0	13.039116262223832	0.0
12	0.0	0.0	0.0	13.88364722926476	0.0
13	0.0	0.0	0.0	14.210068815646505	0.0
14	0.0	0.0	0.0	14.454817095255342	0.0
15	0.0	0.0	0.0	15.32433900760594	0.0
16	0.0	0.0	0.0	16.25172039116262	0.0
17	0.0	0.0	0.0	17.607026439695762	0.0
18	0.0	0.0	0.0	18.150579500181095	0.0
19	0.0	0.0	0.0	18.876403477001087	9.054690329590728E-5
20	0.0	0.0	0.0	19.68317638536762	9.054690329590728E-5
21	9.054690329590728E-5	0.0	0.0	20.354038391887	9.054690329590728E-5
22	9.054690329590728E-5	0.0	0.0	21.164252082578777	9.054690329590728E-5
23	9.054690329590728E-5	0.0	0.0	21.734335385729807	9.054690329590728E-5
24	1.8109380659181456E-4	0.0	0.0	22.202825063382832	9.054690329590728E-5
25	1.8109380659181456E-4	0.0	0.0	22.681455994205	9.054690329590728E-5
26	1.8109380659181456E-4	0.0	0.0	23.100235421948568	9.054690329590728E-5
27	1.8109380659181456E-4	0.0	0.0	23.553513219847883	9.054690329590728E-5
28	1.8109380659181456E-4	0.0	0.0	23.981981166244115	9.054690329590728E-5
29	1.8109380659181456E-4	0.0	0.0	24.570173850054328	9.054690329590728E-5
30	1.8109380659181456E-4	0.0	0.0	25.097700108656284	9.054690329590728E-5
31	1.8109380659181456E-4	0.0	0.0	25.565555957986238	9.054690329590728E-5
32	1.8109380659181456E-4	0.0	0.0	26.00063382832307	9.054690329590728E-5
33	3.621876131836291E-4	0.0	0.0	26.422401303875407	9.054690329590728E-5
34	3.621876131836291E-4	0.0	0.0	27.021821803694312	9.054690329590728E-5
35	3.621876131836291E-4	0.0	0.0	27.457171314741036	9.054690329590728E-5
36	3.621876131836291E-4	0.0	0.0	27.93915248098515	9.054690329590728E-5
37	3.621876131836291E-4	0.0	0.0	28.376675117710974	9.054690329590728E-5
38	3.621876131836291E-4	0.0	0.0	28.78585657370518	9.054690329590728E-5
39	3.621876131836291E-4	0.0	0.0	29.244386091995654	9.054690329590728E-5
40	3.621876131836291E-4	0.0	0.0	29.709163346613547	9.054690329590728E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	20	6.311347E-4	46.0	1
CACGACG	30	1.8613355E-6	46.0	25
AATATCG	20	6.311347E-4	46.0	28
TGTTCGG	20	6.311347E-4	46.0	1
ACTACGC	20	6.311347E-4	46.0	10
ATCCACG	25	3.417069E-5	46.0	34
GCGATAT	40	5.6115823E-9	46.0	8
CGTACGA	30	1.8613355E-6	46.0	16
CTACCGA	20	6.311347E-4	46.0	21
ACTCGAA	20	6.311347E-4	46.0	40
CGTAAGG	25	3.417069E-5	46.0	1
TGGGCGA	220	0.0	46.0	5
TAAGACG	20	6.311347E-4	46.0	25
TAATGCG	25	3.417069E-5	46.0	1
CGATACG	25	3.417069E-5	46.0	9
AATACGG	60	0.0	46.0	1
TCGAATA	20	6.311347E-4	46.0	32
AGTACGT	20	6.311347E-4	46.0	16
CGCCCGA	20	6.311347E-4	46.0	34
GTACGTC	20	6.311347E-4	46.0	17
>>END_MODULE