FastQCFastQC Report
Thu 26 May 2016
SRR1544967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544967_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences694638
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT35990.5181115919370952Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT26330.37904635220071464Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA24620.35442921348961615No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT23940.3446399419553782Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTTG15390.22155424839988597Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.211189137363634No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9860.14194443724645067No Hit
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC8810.1268286503185832No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT8660.12466925218603071Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC7910.11387226152326822No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC7350.10581050849507226No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG206.3097203E-446.00000443
CACGACG206.3097203E-446.00000425
GTTACGG405.6061253E-946.0000041
AACTCGC206.3097203E-446.00000438
ATAGTCA206.3097203E-446.00000420
TAGCTAC206.3097203E-446.00000413
CGATACG206.3097203E-446.0000049
ATACCGG206.3097203E-446.0000041
TACGTAT206.3097203E-446.00000413
CTCACGA253.4157507E-546.023
ATAGGCG253.4157507E-546.011
AACGGCT253.4157507E-546.03
CTAACGG600.046.01
GCGATAT253.4157507E-546.08
CCGGTAT501.6370905E-1146.042
ATCGGAA253.4157507E-546.026
CACAACG301.8603314E-646.011
CTTACGA301.8603314E-646.021
CTAGCGG1350.044.29631
TACGCGG1250.044.161
Produced by FastQC (version 0.11.5)