##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544967_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	694638
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.889096191109616	34.0	33.0	34.0	31.0	34.0
2	33.25781774103922	34.0	33.0	34.0	31.0	34.0
3	33.38076379351547	34.0	34.0	34.0	31.0	34.0
4	36.59812161154443	37.0	37.0	37.0	35.0	37.0
5	36.6056881426009	37.0	37.0	37.0	35.0	37.0
6	36.39561469427241	37.0	37.0	37.0	35.0	37.0
7	36.19615396796605	37.0	37.0	37.0	35.0	37.0
8	36.35499929459661	37.0	37.0	37.0	35.0	37.0
9	38.25824386227071	39.0	39.0	39.0	37.0	39.0
10	38.17118700675748	39.0	39.0	39.0	35.0	39.0
11	37.62716263722975	39.0	37.0	39.0	35.0	39.0
12	37.60721411728123	39.0	37.0	39.0	35.0	39.0
13	37.59885868610701	39.0	37.0	39.0	35.0	39.0
14	38.87555676481851	41.0	37.0	41.0	35.0	41.0
15	38.90891946596645	41.0	37.0	41.0	35.0	41.0
16	38.944064390373114	41.0	37.0	41.0	35.0	41.0
17	39.00961220088737	41.0	37.0	41.0	35.0	41.0
18	39.015469928221606	41.0	37.0	41.0	35.0	41.0
19	38.97828509237905	41.0	37.0	41.0	35.0	41.0
20	38.899775134674464	41.0	36.0	41.0	35.0	41.0
21	38.84663090703359	41.0	35.0	41.0	35.0	41.0
22	38.81653897425709	41.0	35.0	41.0	35.0	41.0
23	38.774884184280154	41.0	35.0	41.0	35.0	41.0
24	38.729217232572935	41.0	35.0	41.0	35.0	41.0
25	38.686079655878316	41.0	35.0	41.0	35.0	41.0
26	38.6158401930214	40.0	35.0	41.0	35.0	41.0
27	38.68404550283745	41.0	36.0	41.0	35.0	41.0
28	38.696212991515004	41.0	36.0	41.0	35.0	41.0
29	38.71976482714738	41.0	36.0	41.0	35.0	41.0
30	38.62732675148783	40.0	36.0	41.0	35.0	41.0
31	38.58233929039298	40.0	35.0	41.0	35.0	41.0
32	38.501433840360015	40.0	35.0	41.0	35.0	41.0
33	38.42103512908882	40.0	35.0	41.0	35.0	41.0
34	38.29448432132996	40.0	35.0	41.0	35.0	41.0
35	38.24082183813727	40.0	35.0	41.0	35.0	41.0
36	38.22356824705818	40.0	35.0	41.0	35.0	41.0
37	38.15536869563715	40.0	35.0	41.0	35.0	41.0
38	38.109277062297195	40.0	35.0	41.0	35.0	41.0
39	38.03693866445544	40.0	35.0	41.0	35.0	41.0
40	37.95758078308414	40.0	35.0	41.0	35.0	41.0
41	37.9096925304979	40.0	35.0	41.0	35.0	41.0
42	37.8338530284839	40.0	35.0	41.0	35.0	41.0
43	37.76659785384618	40.0	35.0	41.0	35.0	41.0
44	37.64137435613946	40.0	35.0	41.0	35.0	41.0
45	37.64137147694195	40.0	35.0	41.0	35.0	41.0
46	37.56542976341634	39.0	35.0	41.0	35.0	41.0
47	37.4545086793409	39.0	35.0	41.0	35.0	41.0
48	37.37840141195846	39.0	35.0	41.0	35.0	41.0
49	37.289232665071594	39.0	35.0	41.0	35.0	41.0
50	37.15851565851566	39.0	35.0	41.0	35.0	41.0
51	36.93525548559106	38.0	35.0	40.0	34.0	41.0
52	36.38844117367607	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	6.0
15	7.0
16	11.0
17	25.0
18	51.0
19	86.0
20	167.0
21	271.0
22	383.0
23	601.0
24	1070.0
25	1814.0
26	2697.0
27	3254.0
28	3329.0
29	3240.0
30	3627.0
31	4441.0
32	5916.0
33	8775.0
34	29084.0
35	122564.0
36	24455.0
37	40562.0
38	81188.0
39	355751.0
40	1256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.176704988785524	21.431162706330493	23.151195298846304	22.24093700603768
2	27.849037916152007	23.95924208004745	25.58526887385948	22.606451129941064
3	27.664913235383032	23.973925987348807	25.071332118311986	23.28982865895618
4	26.128717403885187	26.891416824302734	22.687068660223023	24.292797111589056
5	23.501305716070817	35.56442348388657	20.672350202551545	20.261920597491066
6	85.25433391205203	3.212752541611602	7.988765371315706	3.5441481750206583
7	84.91818760275136	3.0326587373567238	6.403335262395665	5.64581839749625
8	73.49929027781377	5.2480572614800804	12.774999352180561	8.477653108525592
9	47.91099824656872	19.884313844045387	15.949314607032727	16.255373302353167
10	37.377742075728655	20.92226454642562	26.40958312099252	15.29041025685321
11	35.22698153570637	17.280943455440102	26.537563450315126	20.954511558538407
12	19.91411353827461	30.07235423342806	30.223512102706735	19.790020125590598
13	15.054603980778477	33.045845461952844	30.25057655930139	21.648973997967285
14	13.373296594773104	22.6820300645804	42.57282210302344	21.371851237623048
15	15.352600923070723	17.668195520544515	44.756549454536035	22.222654101848732
16	16.285029036706888	21.489178536158402	28.196701015492962	34.02909141164175
17	18.05098482950832	23.268378637506153	33.72461627495185	24.956020258033682
18	23.269386356634676	26.527774178780888	28.22016647520003	21.9826729893844
19	25.27388366314541	21.811792617161746	26.590396724624917	26.323926995067936
20	20.308275677403195	29.55237116310942	27.06056967801934	23.078783481468047
21	20.84251077539668	23.620504492987713	25.892911127810457	29.64407360380515
22	18.428015743451983	29.565615471655743	24.026183422156578	27.9801853627357
23	18.676490488571027	22.227260817864845	36.059789415494116	23.036459278070016
24	20.14934397484733	22.463786893317096	32.20857482602449	25.178294305811082
25	18.053864027018392	28.68530083295184	29.027925336650167	24.232909803379602
26	20.11306608622045	27.318977654548128	27.013350838854194	25.55460542037723
27	20.938963891984024	25.151517768967434	34.23437819410974	19.6751401449388
28	17.91897362367161	24.56013060039906	30.590033945738643	26.93086183019069
29	21.980081711625335	25.51933525087888	27.76510930873347	24.73547372876232
30	25.444907995243565	23.48503825013892	25.661855527627342	25.408198226990176
31	28.559335941886278	25.70072469401328	23.182002712204056	22.557936651896384
32	26.02002769788005	24.014090792614283	24.541847696210112	25.42403381329556
33	21.31412332754615	24.369959604858934	29.06521094440557	25.250706123189342
34	20.54638531148598	23.044664990973715	28.660827654116243	27.748122043424058
35	27.96449373630582	22.32875253009481	26.152038903716758	23.554714829882613
36	23.94038333635649	24.63959645167699	26.83340099447482	24.5866192174917
37	22.117131513104667	25.631336034020595	28.10730193280529	24.144230520069446
38	23.34064649500891	24.99676090280117	24.239531957652762	27.423060644537156
39	29.678623973925987	20.367155266484126	26.5198563856282	23.43436437396169
40	20.058649253280127	19.48007451363156	30.718302194812264	29.742974038276053
41	24.120333180735866	24.148261396583546	23.96456859544108	27.766836827239512
42	23.202157094774545	21.796676830233878	28.214839959806405	26.786326115185176
43	24.078872736590856	25.523222167517467	27.595092695763835	22.802812400127838
44	19.265574299131348	26.968579317572605	27.34848942902634	26.417356954269707
45	22.97930720749513	24.169711418033565	27.312355500274965	25.53862587419634
46	25.07853011208716	20.84481413340474	27.67700586492533	26.399649889582776
47	23.7021297423982	19.23850984253669	31.014139738971952	26.045220676093162
48	21.331254552731064	19.124349661262414	28.498872794174808	31.045522991831714
49	21.42655599031438	22.281965570556174	30.707793123900508	25.583685315228937
50	20.86093763946113	22.800509042119778	28.767933801490848	27.57061951692824
51	21.064928783049588	19.59236321652429	30.374957891736415	28.967750108689707
52	23.130609036649304	18.516838986637644	32.69228000771625	25.6602719689968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	238.0
2	390.0
3	1499.0
4	2608.0
5	1810.5
6	1013.0
7	1073.5
8	1134.0
9	1152.5
10	1171.0
11	1151.0
12	1131.0
13	1068.0
14	989.5
15	974.0
16	961.0
17	948.0
18	949.0
19	950.0
20	1146.0
21	1342.0
22	1385.5
23	1429.0
24	1580.5
25	1732.0
26	1779.5
27	1827.0
28	2799.5
29	3772.0
30	4235.5
31	4699.0
32	5547.0
33	6395.0
34	7710.5
35	9026.0
36	10340.5
37	11655.0
38	12713.5
39	16067.5
40	18363.0
41	23587.0
42	28811.0
43	37073.0
44	45335.0
45	50526.0
46	55717.0
47	58022.0
48	60327.0
49	69860.0
50	79393.0
51	82422.5
52	85452.0
53	75220.0
54	64988.0
55	54847.5
56	44707.0
57	38323.5
58	31940.0
59	29683.0
60	27426.0
61	25853.5
62	24281.0
63	22676.5
64	17592.0
65	14112.0
66	11292.0
67	8472.0
68	7269.5
69	6067.0
70	5168.0
71	4269.0
72	3439.5
73	2610.0
74	2309.0
75	2008.0
76	1623.0
77	1238.0
78	830.5
79	423.0
80	379.0
81	335.0
82	245.5
83	156.0
84	107.0
85	58.0
86	35.5
87	13.0
88	9.5
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	694638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.647843303555042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.74616811473395	18.852328022483924
2	11.705912386898293	6.238746380224193
3	4.3006152164734335	3.4380636119240555
4	1.9847509840946513	2.115573328829237
5	1.1192762252597317	1.4913148732057955
6	0.71422667393204	1.1419560294096183
7	0.5705070344701272	1.0641947440695076
8	0.4577072887479101	0.9757529687559465
9	0.37555272920185734	0.900690325199418
>10	7.555136007837872	49.77950426926433
>50	0.3713403919532248	6.5690257623116
>100	0.08849193550931456	4.1038508115374785
>500	0.0070576390283502415	1.2900214223033701
>1k	0.0032573718592385733	2.0389774504815183
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	3599	0.5181115919370952	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT	2633	0.37904635220071464	Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2462	0.35442921348961615	No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	2394	0.3446399419553782	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTTG	1539	0.22155424839988597	Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1467	0.211189137363634	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	986	0.14194443724645067	No Hit
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	881	0.1268286503185832	No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	866	0.12466925218603071	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	791	0.11387226152326822	No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	735	0.10581050849507226	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3862443459758896	0.0
2	0.0	0.0	0.0	1.4908484707142424	0.0
3	0.0	0.0	0.0	2.29371269639726	0.0
4	0.0	0.0	0.0	2.823772958001146	0.0
5	0.0	0.0	0.0	5.626095894552271	0.0
6	0.0	0.0	0.0	6.133410495826603	0.0
7	0.0	0.0	0.0	8.647381801744217	0.0
8	0.0	0.0	0.0	11.233764925040093	0.0
9	0.0	0.0	0.0	13.7104506232023	0.0
10	0.0	0.0	0.0	14.875949775278634	0.0
11	0.0	0.0	0.0	15.98602437528612	0.0
12	0.0	0.0	0.0	16.958617294187764	0.0
13	1.4395987550349968E-4	0.0	0.0	17.334496529127403	0.0
14	2.8791975100699935E-4	0.0	0.0	17.62270419988541	0.0
15	2.8791975100699935E-4	0.0	0.0	18.599184035425647	0.0
16	4.31879626510499E-4	0.0	0.0	19.599705170174968	0.0
17	4.31879626510499E-4	0.0	0.0	20.97653741949044	0.0
18	4.31879626510499E-4	0.0	0.0	21.551369202375913	0.0
19	4.31879626510499E-4	0.0	0.0	22.296793437733037	0.0
20	4.31879626510499E-4	0.0	0.0	23.1550822154849	0.0
21	4.31879626510499E-4	0.0	0.0	23.882079586777575	0.0
22	4.31879626510499E-4	0.0	0.0	24.73273849112775	0.0
23	4.31879626510499E-4	0.0	0.0	25.34672736015018	0.0
24	4.31879626510499E-4	0.0	0.0	25.851738603416457	0.0
25	4.31879626510499E-4	0.0	0.0	26.36538743921294	0.0
26	4.31879626510499E-4	0.0	0.0	26.81943688655098	0.0
27	4.31879626510499E-4	0.0	0.0	27.304149787371262	0.0
28	4.31879626510499E-4	0.0	0.0	27.751001240934126	0.0
29	4.31879626510499E-4	0.0	0.0	28.364126350703533	0.0
30	5.758395020139987E-4	0.0	0.0	28.9098782387373	0.0
31	5.758395020139987E-4	0.0	0.0	29.38451394827234	0.0
32	5.758395020139987E-4	0.0	0.0	29.832805000590234	0.0
33	5.758395020139987E-4	0.0	0.0	30.265692346229258	0.0
34	5.758395020139987E-4	0.0	0.0	30.85693555492213	0.0
35	5.758395020139987E-4	0.0	0.0	31.30637828624406	0.0
36	5.758395020139987E-4	0.0	0.0	31.785044872293195	0.0
37	5.758395020139987E-4	0.0	0.0	32.21361342166711	0.0
38	5.758395020139987E-4	0.0	0.0	32.621451748968525	0.0
39	5.758395020139987E-4	0.0	0.0	33.06916696178441	0.0
40	5.758395020139987E-4	0.0	0.0	33.52825500476507	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	20	6.3097203E-4	46.000004	43
CACGACG	20	6.3097203E-4	46.000004	25
GTTACGG	40	5.6061253E-9	46.000004	1
AACTCGC	20	6.3097203E-4	46.000004	38
ATAGTCA	20	6.3097203E-4	46.000004	20
TAGCTAC	20	6.3097203E-4	46.000004	13
CGATACG	20	6.3097203E-4	46.000004	9
ATACCGG	20	6.3097203E-4	46.000004	1
TACGTAT	20	6.3097203E-4	46.000004	13
CTCACGA	25	3.4157507E-5	46.0	23
ATAGGCG	25	3.4157507E-5	46.0	11
AACGGCT	25	3.4157507E-5	46.0	3
CTAACGG	60	0.0	46.0	1
GCGATAT	25	3.4157507E-5	46.0	8
CCGGTAT	50	1.6370905E-11	46.0	42
ATCGGAA	25	3.4157507E-5	46.0	26
CACAACG	30	1.8603314E-6	46.0	11
CTTACGA	30	1.8603314E-6	46.0	21
CTAGCGG	135	0.0	44.2963	1
TACGCGG	125	0.0	44.16	1
>>END_MODULE