FastQCFastQC Report
Thu 26 May 2016
SRR1544966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544966_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1053172
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT35520.3372668472006472No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT30530.2898861724390698No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT28740.2728898983261993No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT28560.27118077578970956Illumina Single End Adapter 2 (95% over 21bp)
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA25270.2399418138727577No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23210.22038185595515264No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA17880.16977283862465012No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16650.15809383462530335No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT15230.14461075683743968No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG14690.13948338922797035No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC11010.10454132848195735No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT10760.10216754718127713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG351.0194526E-746.00000445
ACGCGAT253.41696E-546.026
AAATACG253.41696E-546.027
GACCGTT301.8612536E-646.08
ATGTCGC253.41696E-546.011
TCGATGG750.046.01
CTTATCG301.8612536E-646.05
ATATACG253.41696E-546.029
CGTACTC206.311213E-446.029
GAACGAT206.311213E-446.08
GCGATAA253.41696E-546.08
CGTACAC253.41696E-546.034
CTCGAGG1150.046.01
TAGATCG206.311213E-446.028
CGGTATG301.8612536E-646.05
AATGCGG2000.044.851
ACGGGAT3700.044.7567564
CTAACGG1550.044.5161321
TAGTAGG3000.044.4666631
TGCACGG1300.044.230771
Produced by FastQC (version 0.11.5)