##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544966_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1053172
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92561423964936	34.0	33.0	34.0	31.0	34.0
2	33.25014812395316	34.0	33.0	34.0	31.0	34.0
3	33.31436365569916	34.0	34.0	34.0	31.0	34.0
4	36.58179575605884	37.0	37.0	37.0	35.0	37.0
5	36.579481794046934	37.0	37.0	37.0	35.0	37.0
6	36.66412039059147	37.0	37.0	37.0	35.0	37.0
7	36.48775033897597	37.0	37.0	37.0	35.0	37.0
8	36.5208569920203	37.0	37.0	37.0	35.0	37.0
9	38.33374890331304	39.0	39.0	39.0	37.0	39.0
10	38.13973501004584	39.0	39.0	39.0	35.0	39.0
11	37.5150117929455	39.0	35.0	39.0	35.0	39.0
12	37.43530021686866	39.0	35.0	39.0	35.0	39.0
13	37.43403831472922	39.0	35.0	39.0	35.0	39.0
14	38.74908087188037	41.0	35.0	41.0	35.0	41.0
15	38.81065485979498	41.0	35.0	41.0	35.0	41.0
16	38.809423342056185	41.0	35.0	41.0	35.0	41.0
17	38.864887216902844	41.0	36.0	41.0	35.0	41.0
18	38.83783655471281	41.0	36.0	41.0	35.0	41.0
19	38.77287660515091	41.0	36.0	41.0	35.0	41.0
20	38.697472967378545	41.0	35.0	41.0	35.0	41.0
21	38.64493169206929	41.0	35.0	41.0	35.0	41.0
22	38.61280113789581	41.0	35.0	41.0	35.0	41.0
23	38.593602944248424	41.0	35.0	41.0	35.0	41.0
24	38.53155325056116	41.0	35.0	41.0	35.0	41.0
25	38.49399718184684	40.0	35.0	41.0	35.0	41.0
26	38.44320680762497	40.0	35.0	41.0	35.0	41.0
27	38.41113892127782	40.0	35.0	41.0	35.0	41.0
28	38.44957044053583	40.0	36.0	41.0	35.0	41.0
29	38.4668572654799	40.0	36.0	41.0	35.0	41.0
30	38.3946743741763	40.0	35.0	41.0	35.0	41.0
31	38.334300570087315	40.0	35.0	41.0	35.0	41.0
32	38.26542483089182	40.0	35.0	41.0	35.0	41.0
33	38.172930917267074	40.0	35.0	41.0	35.0	41.0
34	38.08289339253228	40.0	35.0	41.0	35.0	41.0
35	37.99414340677496	40.0	35.0	41.0	35.0	41.0
36	37.92542908470791	40.0	35.0	41.0	35.0	41.0
37	37.86471630464919	40.0	35.0	41.0	35.0	41.0
38	37.79922937563855	40.0	35.0	41.0	35.0	41.0
39	37.69435571777449	40.0	35.0	41.0	35.0	41.0
40	37.61104359022078	40.0	35.0	41.0	35.0	41.0
41	37.57450824746575	40.0	35.0	41.0	35.0	41.0
42	37.48671347130383	39.0	35.0	41.0	35.0	41.0
43	37.406312549137276	39.0	35.0	41.0	34.0	41.0
44	37.33185082778502	39.0	35.0	41.0	34.0	41.0
45	37.27648855077803	39.0	35.0	41.0	34.0	41.0
46	37.27186632382934	39.0	35.0	41.0	34.0	41.0
47	37.20027972638847	39.0	35.0	41.0	34.0	41.0
48	37.20223002510511	39.0	35.0	41.0	34.0	41.0
49	37.13721595332956	39.0	35.0	41.0	34.0	41.0
50	37.04904042264701	38.0	35.0	41.0	34.0	41.0
51	36.97531457349797	38.0	35.0	41.0	34.0	41.0
52	36.76076747197988	37.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	4.0
14	15.0
15	17.0
16	26.0
17	51.0
18	68.0
19	159.0
20	275.0
21	441.0
22	683.0
23	1177.0
24	1785.0
25	3106.0
26	4401.0
27	5304.0
28	5421.0
29	5913.0
30	6895.0
31	8523.0
32	11008.0
33	15896.0
34	49784.0
35	203111.0
36	39390.0
37	62163.0
38	108778.0
39	513339.0
40	5433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.579130474414434	22.239292347308893	23.888215790013405	20.293361388263264
2	27.334471482340966	24.625132456996578	25.841457995465127	22.198938065197325
3	27.766024922804633	24.56749704701606	25.220002050947045	22.44647597923226
4	26.52767069386577	27.236861595256993	22.091548199154552	24.143919511722682
5	23.803994029465272	36.45786253337536	20.068516823462833	19.66962661369653
6	87.02794985054673	3.456320525042443	6.8642159115510095	2.651513712859818
7	86.69514571219136	3.281515270060351	5.390762382592777	4.632576635155511
8	75.98606875230257	6.015541620931813	10.615360074137937	7.38302955262768
9	49.42412065645498	22.1461451690702	14.66541077810652	13.764323396368304
10	39.4863327167832	20.726339097507342	25.26700292070051	14.520325265008942
11	39.27459142476253	17.21200335747627	24.98062994458645	18.53277527317475
12	21.387294762868745	32.13682095612113	27.34833436513694	19.12754991587319
13	15.096014706049916	33.04379531548503	29.69619397401374	22.163996004451313
14	13.776477156627786	23.957910009001377	41.591212071722374	20.674400762648457
15	16.373298948319935	18.04861883908801	43.47751364449491	22.100568568097138
16	16.918224183704087	22.222485975700078	27.79270622462428	33.06658361597156
17	20.067852164698643	23.10857105961799	31.861082520234113	24.96249425544925
18	22.879453688476335	27.077533394355335	26.769036776518934	23.273976140649392
19	25.874785884926677	23.048087112076658	26.255635356807815	24.821491646188846
20	20.76821259965134	29.750221236417225	26.934726711306418	22.546839452625022
21	20.8277470346724	24.39392615831032	25.26405943188767	29.51426737512961
22	19.759830303122378	27.818343062671623	24.165853250940966	28.255973383265033
23	19.10010900403733	22.04378771938487	36.35142217985287	22.50468109672494
24	19.70732226075133	22.418845164892346	32.466016946899465	25.407815627456863
25	17.13661206336667	29.47239387298561	28.4973394659182	24.893654597729526
26	17.872959022837676	29.466886700368033	27.33940894744638	25.32074532934791
27	19.108559665467748	27.120451360271637	33.61597156020099	20.15501741405962
28	17.260998203522313	25.089064274401522	29.658593278210965	27.9913442438652
29	19.348216625584424	30.1584166688822	26.324475014527543	24.168891691005836
30	25.769010190168366	24.59987542395734	22.723733635151714	26.90738075072258
31	26.82429840519877	28.0140375930997	22.663914346374572	22.497749655326952
32	25.61651847941267	28.34817104898345	21.737000224084955	24.298310247518923
33	18.19493871846194	27.936652322697526	24.057134067369812	29.811274891470717
34	22.235304394723748	26.041615234738487	26.333970139730262	25.389110230807503
35	27.152260029700752	26.908045409486768	22.591941297337947	23.347753263474534
36	23.275970116941963	29.567535027516872	24.123694895040888	23.03279996050028
37	21.70053894330651	29.204156586008743	25.400884186058875	23.69442028462587
38	21.282088775622597	30.84320509850243	23.726039051550934	24.14866707432404
39	25.900992430486188	26.370336469256685	25.136255046659045	22.592416053598082
40	17.46647271290919	23.610578329085847	29.542657799485745	29.380291158519217
41	20.97881447664769	26.79638273710277	23.721196537697544	28.503606248551993
42	21.746495349287677	24.05286126102859	26.333210529714044	27.867432859969693
43	22.379535346553077	28.00881527423821	26.01854208049587	23.59310729871284
44	18.75534100792653	29.98826402524944	25.674058938141158	25.582336028682874
45	22.505915463001294	27.72453122566874	24.658175492702046	25.11137781862792
46	23.27729943447034	25.03171371817709	26.394359136019567	25.296627711333002
47	21.18153539972578	23.07353404761995	30.528631600536283	25.21629895211798
48	20.838096721143366	22.737311664191605	27.84151116816626	28.583080446498766
49	20.88690166468535	25.001139415024326	29.88818540561276	24.223773514677564
50	19.51922383048543	26.864652687310336	27.221479492428585	26.394643989775652
51	20.564067407792837	22.262555404055558	29.778231855765252	27.39514533238635
52	21.909051892758256	21.698924772022043	30.993323027957448	25.39870030726225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	153.0
1	386.5
2	620.0
3	2433.0
4	4246.0
5	2845.5
6	1445.0
7	1534.5
8	1624.0
9	1562.0
10	1500.0
11	1460.0
12	1420.0
13	1387.0
14	1355.5
15	1357.0
16	1279.5
17	1202.0
18	1240.5
19	1279.0
20	1388.0
21	1497.0
22	1511.0
23	1525.0
24	2217.0
25	2909.0
26	4106.0
27	5303.0
28	8862.5
29	12422.0
30	11390.0
31	10358.0
32	11566.0
33	12774.0
34	16147.0
35	19520.0
36	20952.0
37	22384.0
38	23903.5
39	29351.0
40	33279.0
41	42161.0
42	51043.0
43	67474.0
44	83905.0
45	93180.5
46	102456.0
47	106502.0
48	110548.0
49	111370.0
50	112192.0
51	99807.5
52	87423.0
53	75554.5
54	63686.0
55	57069.0
56	50452.0
57	46348.5
58	42245.0
59	41269.5
60	40294.0
61	39586.5
62	38879.0
63	36781.0
64	28352.0
65	22021.0
66	18916.5
67	15812.0
68	13813.0
69	11814.0
70	9831.5
71	7849.0
72	6748.0
73	5647.0
74	4746.5
75	3846.0
76	3098.5
77	2351.0
78	1824.0
79	1297.0
80	1016.5
81	736.0
82	491.0
83	246.0
84	179.0
85	112.0
86	68.5
87	25.0
88	14.5
89	5.5
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1053172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.25355625710079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.66898244085861	15.503826201226142
2	12.08701415405497	5.379580989152716
3	4.66171523771998	3.1121922689155666
4	2.230843928444972	1.9857684344984767
5	1.2591699318076963	1.4010504457366169
6	0.8436866429784602	1.1265016903731402
7	0.560103848952721	0.8725011758741642
8	0.483721713959723	0.8611622699507129
9	0.36004897707077865	0.7211133149900548
>10	6.091747630037977	36.34598972164556
>50	1.5365711203292842	21.61989089115417
>100	0.20270935409882315	7.280417872759967
>500	0.00812548043856042	1.2380517138135216
>1k	0.005559539247436077	2.551953009909262
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	3552	0.3372668472006472	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	3053	0.2898861724390698	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	2874	0.2728898983261993	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT	2856	0.27118077578970956	Illumina Single End Adapter 2 (95% over 21bp)
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	2527	0.2399418138727577	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2321	0.22038185595515264	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1788	0.16977283862465012	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1665	0.15809383462530335	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	1523	0.14461075683743968	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	1469	0.13948338922797035	No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	1101	0.10454132848195735	No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	1076	0.10216754718127713	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27573843588701563	0.0
2	0.0	0.0	0.0	1.078266418020988	0.0
3	0.0	0.0	0.0	1.7463434272844323	0.0
4	0.0	0.0	0.0	2.139821415685187	0.0
5	0.0	0.0	0.0	4.430330468337555	0.0
6	0.0	0.0	0.0	4.863213226329602	0.0
7	0.0	0.0	0.0	6.8269950207563435	0.0
8	0.0	0.0	0.0	8.932444083207681	0.0
9	0.0	0.0	0.0	10.810484897053852	0.0
10	0.0	0.0	0.0	11.70407112988192	0.0
11	0.0	0.0	0.0	12.674188071843915	0.0
12	0.0	0.0	0.0	13.46028948737718	0.0
13	0.0	0.0	0.0	13.770400276498046	0.0
14	0.0	0.0	0.0	14.019267508061361	0.0
15	0.0	0.0	0.0	14.812870072504776	0.0
16	0.0	0.0	0.0	15.690694397496326	0.0
17	0.0	0.0	0.0	16.885940758014836	0.0
18	0.0	0.0	0.0	17.415958646830717	0.0
19	0.0	0.0	0.0	18.07995275225699	0.0
20	0.0	0.0	0.0	18.82000281055706	0.0
21	0.0	0.0	0.0	19.50858929025838	0.0
22	0.0	0.0	0.0	20.224331828039485	0.0
23	0.0	0.0	0.0	20.772010649732426	0.0
24	0.0	0.0	0.0	21.27069462537933	0.0
25	9.495125202720923E-5	0.0	0.0	21.718389778687623	0.0
26	1.8990250405441847E-4	0.0	0.0	22.141967313981002	0.0
27	2.848537560816277E-4	0.0	0.0	22.553770894023007	0.0
28	2.848537560816277E-4	0.0	0.0	22.96775835286164	0.0
29	2.848537560816277E-4	0.0	0.0	23.513063393253905	0.0
30	2.848537560816277E-4	0.0	0.0	24.033871010623145	0.0
31	2.848537560816277E-4	0.0	0.0	24.489067312841588	0.0
32	2.848537560816277E-4	0.0	0.0	24.921950070833635	0.0
33	2.848537560816277E-4	0.0	0.0	25.336792090940513	0.0
34	3.7980500810883694E-4	0.0	0.0	25.911342116957155	0.0
35	3.7980500810883694E-4	0.0	0.0	26.348117876282316	0.0
36	3.7980500810883694E-4	0.0	0.0	26.774448997884488	0.0
37	3.7980500810883694E-4	0.0	0.0	27.191474896787987	0.0
38	4.747562601360462E-4	0.0	0.0	27.597961206716473	0.0
39	5.697075121632554E-4	0.0	0.0	28.049644312609907	0.0
40	5.697075121632554E-4	0.0	0.0	28.51395593502296	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAACG	35	1.0194526E-7	46.000004	45
ACGCGAT	25	3.41696E-5	46.0	26
AAATACG	25	3.41696E-5	46.0	27
GACCGTT	30	1.8612536E-6	46.0	8
ATGTCGC	25	3.41696E-5	46.0	11
TCGATGG	75	0.0	46.0	1
CTTATCG	30	1.8612536E-6	46.0	5
ATATACG	25	3.41696E-5	46.0	29
CGTACTC	20	6.311213E-4	46.0	29
GAACGAT	20	6.311213E-4	46.0	8
GCGATAA	25	3.41696E-5	46.0	8
CGTACAC	25	3.41696E-5	46.0	34
CTCGAGG	115	0.0	46.0	1
TAGATCG	20	6.311213E-4	46.0	28
CGGTATG	30	1.8612536E-6	46.0	5
AATGCGG	200	0.0	44.85	1
ACGGGAT	370	0.0	44.756756	4
CTAACGG	155	0.0	44.516132	1
TAGTAGG	300	0.0	44.466663	1
TGCACGG	130	0.0	44.23077	1
>>END_MODULE