FastQCFastQC Report
Thu 26 May 2016
SRR1544965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544965_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences670533
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT31580.47096861750279256No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT23570.35151140958014Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA22850.3407736830252948No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT21820.32541276864822466No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.22907149983669708No Hit
TGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTTG14460.21564934164314062Illumina Single End Adapter 2 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT12820.19119118671265994No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA10960.16345205977930988No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10740.16017108777644054No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8660.12915098884022114No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC8010.11945720792265258No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT7790.11617623591978322No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT7680.11453574991834854No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC7350.1096142919140445No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG7160.1067807251842937No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC6980.1040962935455824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG351.018725E-746.00000445
CTCACGT301.860235E-646.044
ATAGCGG253.415622E-546.01
ATTAACG206.309564E-446.010
AGGGTTG600.046.05
ACGGGTA206.309564E-446.04
CTAACGG850.046.01
CGAGGGT206.309564E-446.03
AGATTGG800.046.01
AACGAGT206.309564E-446.029
GTCCCGT206.309564E-446.017
TATCGGC206.309564E-446.012
ATCGAGG800.046.01
TCTATCC253.415622E-546.022
CCGCAAT551.8189894E-1245.99999632
ACGGGAT2900.045.2068984
TTACGGG1850.044.7567562
CGTTTTT11050.044.1266941
CACGAGG1100.043.909091
CGGGATA1050.043.8095255
Produced by FastQC (version 0.11.5)