##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544965_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	670533
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89946803513026	34.0	33.0	34.0	31.0	34.0
2	33.26984055967417	34.0	33.0	34.0	31.0	34.0
3	33.400060847117146	34.0	34.0	34.0	31.0	34.0
4	36.61215928224264	37.0	37.0	37.0	35.0	37.0
5	36.621511543801724	37.0	37.0	37.0	35.0	37.0
6	36.4134248426252	37.0	37.0	37.0	35.0	37.0
7	36.22882691828739	37.0	37.0	37.0	35.0	37.0
8	36.36529447469401	37.0	37.0	37.0	35.0	37.0
9	38.26977195753229	39.0	39.0	39.0	37.0	39.0
10	38.14986883568743	39.0	39.0	39.0	35.0	39.0
11	37.595247362918755	39.0	35.0	39.0	35.0	39.0
12	37.57339608937964	39.0	35.0	39.0	35.0	39.0
13	37.576020866981935	39.0	35.0	39.0	35.0	39.0
14	38.82647535617188	41.0	36.0	41.0	35.0	41.0
15	38.85893460873663	41.0	36.0	41.0	35.0	41.0
16	38.88227275913341	41.0	36.0	41.0	35.0	41.0
17	38.964513305087145	41.0	36.0	41.0	35.0	41.0
18	38.955632310415744	41.0	36.0	41.0	35.0	41.0
19	38.92071382019975	41.0	36.0	41.0	35.0	41.0
20	38.86045727801614	41.0	35.0	41.0	35.0	41.0
21	38.80514605545141	41.0	35.0	41.0	35.0	41.0
22	38.77570082307657	41.0	35.0	41.0	35.0	41.0
23	38.73713299718284	41.0	35.0	41.0	35.0	41.0
24	38.70089913546388	41.0	35.0	41.0	35.0	41.0
25	38.65323854306947	41.0	35.0	41.0	35.0	41.0
26	38.58651550333839	41.0	35.0	41.0	35.0	41.0
27	38.65774391416977	41.0	36.0	41.0	35.0	41.0
28	38.68483579480801	41.0	36.0	41.0	35.0	41.0
29	38.68060632362613	41.0	36.0	41.0	35.0	41.0
30	38.6228418288138	41.0	35.0	41.0	35.0	41.0
31	38.56483126110124	41.0	35.0	41.0	35.0	41.0
32	38.506549267523	40.0	35.0	41.0	35.0	41.0
33	38.4111445670832	40.0	35.0	41.0	35.0	41.0
34	38.33996984488459	40.0	35.0	41.0	35.0	41.0
35	38.23259705338887	40.0	35.0	41.0	35.0	41.0
36	38.19465261217569	40.0	35.0	41.0	35.0	41.0
37	38.12778342005539	40.0	35.0	41.0	35.0	41.0
38	38.07037834081246	40.0	35.0	41.0	35.0	41.0
39	38.003377909812045	40.0	35.0	41.0	35.0	41.0
40	37.938766622970086	40.0	35.0	41.0	35.0	41.0
41	37.90504717888605	40.0	35.0	41.0	35.0	41.0
42	37.83903849624105	40.0	35.0	41.0	35.0	41.0
43	37.77047960353927	40.0	35.0	41.0	35.0	41.0
44	37.57211054489488	40.0	35.0	41.0	35.0	41.0
45	37.58821415202533	40.0	35.0	41.0	35.0	41.0
46	37.545437733862464	39.0	35.0	41.0	35.0	41.0
47	37.451643692405895	39.0	35.0	41.0	35.0	41.0
48	37.40769805512928	39.0	35.0	41.0	35.0	41.0
49	37.35420628067522	39.0	35.0	41.0	35.0	41.0
50	37.27650838959455	39.0	35.0	41.0	35.0	41.0
51	37.19441847008275	39.0	35.0	41.0	34.0	41.0
52	36.71887587933778	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	2.0
15	6.0
16	13.0
17	30.0
18	45.0
19	87.0
20	144.0
21	234.0
22	372.0
23	551.0
24	999.0
25	1622.0
26	2480.0
27	2960.0
28	2767.0
29	2981.0
30	3410.0
31	4256.0
32	5503.0
33	8832.0
34	29820.0
35	123367.0
36	24462.0
37	40035.0
38	72104.0
39	341080.0
40	2369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.654917804194575	21.82114825071995	23.39944491919115	22.124489025894327
2	27.246235457464437	24.635625688817704	25.851374950971838	22.266763902746025
3	28.005631341037653	24.36181366166915	25.331639158699126	22.300915838594072
4	26.53441366793282	26.626728289286284	22.529241662975576	24.30961637980532
5	23.940507029482518	35.81300249204737	20.24255331206667	20.003937166403443
6	85.794584308304	3.2466709319302707	7.62527720485047	3.333467554915269
7	85.49512104549665	3.0187925128218893	6.087097875868898	5.39898856581257
8	74.04094951329763	5.95093753774982	11.782716137759067	8.225396811193484
9	48.87499944074341	21.26054944350241	15.300365530108136	14.564085585646044
10	38.30236543167898	20.172161549096018	26.570653495055424	14.95481952416958
11	37.61172082507498	17.067318088744326	25.691949538650594	19.629011547530098
12	21.156304014865785	30.451446834085722	28.4971806011039	19.895068549944597
13	15.169275785084402	31.70507640936389	30.234306141532187	22.891341664019517
14	13.796934677338774	23.441053609591176	41.06091721063691	21.701094502433136
15	16.415001200537482	17.673104828546844	43.17893377358013	22.732960197335554
16	16.753239587015106	22.586658673025788	28.248572404341026	32.411529335618084
17	19.591131234406063	23.189164440825433	32.11564531499568	25.104059009772822
18	22.45407757709166	27.166299048667252	27.123646412629952	23.255976961611136
19	25.17788087983738	23.01452724921816	26.257171533690364	25.550420337254092
20	20.3808015414603	30.09725099286687	26.8619143278556	22.66003313781723
21	20.4555182220711	24.24817272229704	25.317471325050374	29.978837730581493
22	19.40337015478731	28.131501357875006	24.265770663039703	28.199357824297984
23	18.706163604177572	22.19696868013953	36.15735541725761	22.93951229842528
24	19.96441636727797	22.57935105356485	31.90327694535541	25.552955633801766
25	17.42106652469006	28.871211409431	28.548035667148376	25.159686398730564
26	18.092174434367884	28.48211795690891	27.19612606687516	26.229581541848052
27	19.97097831128371	26.271637637521195	32.96511879355677	20.792265257638327
28	17.83059148468457	24.90078788068596	29.086562480892063	28.1820581537374
29	20.130105453422875	29.53888921201492	25.698511482656333	24.63249385190587
30	25.74698038724418	24.381648628777405	21.91913000553291	27.952240978445502
31	26.989424830694386	28.066478458181777	21.63622073783095	23.307875973292887
32	25.46541333536157	28.049626192894312	21.554196437759217	24.930764033984904
33	17.869217473263806	27.854706628905664	23.768255999331874	30.507819898498656
34	23.069110692538622	25.64765641661186	26.03346889713109	25.24976399371843
35	26.63388677365618	26.978090563775385	22.467052330012095	23.92097033255634
36	24.165253611679066	29.44970642757329	22.801264069031653	23.583775891715995
37	22.211136513788286	28.97381635206619	25.133736892889686	23.681310241255837
38	21.59938437034419	30.70109897648587	22.73117057624308	24.968346076926863
39	26.213922357288904	26.362460907964262	24.772382567300937	22.651234167445896
40	17.4148028508664	23.415998914296537	29.105353502363045	30.063844732474017
41	21.04982155986357	27.109776849163275	23.217201837940863	28.623199753032292
42	21.478734081693222	23.737832440759814	26.41480732491913	28.368626152627836
43	23.06955809781174	27.43936540036061	25.86643759516683	23.62463890666082
44	19.134777855825142	28.783669110990807	26.15218042959854	25.929372603585506
45	22.271983630932407	27.054447730387615	24.85470513755475	25.81886350112522
46	23.642087712312442	23.953630917493992	26.36320658341946	26.041074786774104
47	20.729926789583807	22.534610526252997	30.866489792448693	25.868972891714503
48	20.764078725431858	21.831885977274794	27.53883850608397	29.86519679120938
49	20.799274606917184	23.858035324137663	30.02656095971414	25.316129109231017
50	19.846450510265715	25.728189365773197	27.674551438929928	26.750808685031163
51	20.734251707223954	21.354057145584186	29.629115942093826	28.28257520509803
52	21.65366954348257	21.318413858825743	31.55116899541111	25.476747602280575
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	244.0
2	411.0
3	1606.5
4	2802.0
5	1930.5
6	1059.0
7	1050.5
8	1042.0
9	1027.0
10	1012.0
11	973.0
12	934.0
13	945.5
14	891.5
15	826.0
16	816.0
17	806.0
18	816.5
19	827.0
20	878.5
21	930.0
22	938.5
23	947.0
24	1331.0
25	1715.0
26	2251.0
27	2787.0
28	4488.5
29	6190.0
30	5992.0
31	5794.0
32	6267.0
33	6740.0
34	8704.5
35	10669.0
36	11637.5
37	12606.0
38	13688.0
39	16951.0
40	19132.0
41	24371.5
42	29611.0
43	39716.0
44	49821.0
45	56365.0
46	62909.0
47	68448.0
48	73987.0
49	75570.5
50	77154.0
51	68612.5
52	60071.0
53	51758.5
54	43446.0
55	38662.0
56	33878.0
57	30491.0
58	27104.0
59	26223.0
60	25342.0
61	24898.0
62	24454.0
63	23990.5
64	18810.5
65	14094.0
66	12208.0
67	10322.0
68	8968.0
69	7614.0
70	6340.0
71	5066.0
72	4309.5
73	3553.0
74	3055.0
75	2557.0
76	2041.5
77	1526.0
78	1173.0
79	820.0
80	622.5
81	425.0
82	290.0
83	155.0
84	101.5
85	48.0
86	29.5
87	11.0
88	7.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	670533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.819950860293957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75717682859604	18.977040055560764
2	11.388568023814386	6.108816695356338
3	4.02423950675604	3.2378971746375176
4	1.838923625952971	1.9727936513556907
5	1.1248267287457892	1.5083898795653632
6	0.7768516497496537	1.2501073843214403
7	0.5915989431826257	1.11066582096119
8	0.5453394944697394	1.1700782755084767
9	0.4789540792058246	1.15609723817738
>10	8.03178025669123	49.361786849019836
>50	0.3371768864429399	6.065453832072394
>100	0.09226233211125097	4.286683949461131
>500	0.007269153439068258	1.332315288518496
>1k	0.005032490842431871	2.4618739054839747
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	3158	0.47096861750279256	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT	2357	0.35151140958014	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2285	0.3407736830252948	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	2182	0.32541276864822466	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1536	0.22907149983669708	No Hit
TGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTTG	1446	0.21564934164314062	Illumina Single End Adapter 2 (95% over 22bp)
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1282	0.19119118671265994	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1096	0.16345205977930988	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1074	0.16017108777644054	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	866	0.12915098884022114	No Hit
ACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	801	0.11945720792265258	No Hit
TCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	779	0.11617623591978322	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	768	0.11453574991834854	No Hit
GCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	735	0.1096142919140445	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	716	0.1067807251842937	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	698	0.1040962935455824	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3576259483127601	0.0
2	0.0	0.0	0.0	1.374279863929143	0.0
3	0.0	0.0	0.0	2.1726000062636737	0.0
4	0.0	0.0	0.0	2.657587322324181	0.0
5	0.0	0.0	0.0	5.336650097758052	0.0
6	0.0	0.0	0.0	5.828646763097416	0.0
7	0.0	0.0	0.0	8.20481616863003	0.0
8	0.0	0.0	0.0	10.674045870971302	0.0
9	0.0	0.0	0.0	12.931354608945421	0.0
10	0.0	0.0	0.0	14.03868265991383	0.0
11	0.0	0.0	0.0	15.122298231406955	0.0
12	0.0	0.0	0.0	16.059612278590315	0.0
13	0.0	0.0	0.0	16.432599141280146	0.0
14	0.0	0.0	0.0	16.71252570716132	0.0
15	0.0	0.0	0.0	17.626574680142514	0.0
16	0.0	0.0	0.0	18.6232444935596	0.0
17	0.0	0.0	0.0	19.854951210454967	0.0
18	0.0	0.0	0.0	20.406751047301178	0.0
19	0.0	0.0	0.0	21.112756568282247	0.0
20	0.0	0.0	0.0	21.893329634783075	0.0
21	0.0	0.0	0.0	22.646312709441595	0.0
22	0.0	0.0	0.0	23.416595454660694	0.0
23	0.0	0.0	0.0	24.030882894652464	0.0
24	0.0	0.0	0.0	24.554496199292206	0.0
25	1.491350910395163E-4	0.0	0.0	25.02725443788747	0.0
26	1.491350910395163E-4	0.0	0.0	25.497924785208184	0.0
27	1.491350910395163E-4	0.0	0.0	25.96203318852316	0.0
28	1.491350910395163E-4	0.0	0.0	26.417640891648883	0.0
29	1.491350910395163E-4	0.0	0.0	26.971528619769646	0.0
30	1.491350910395163E-4	0.0	0.0	27.514380351153484	0.0
31	1.491350910395163E-4	0.0	0.0	27.99727977593944	0.0
32	1.491350910395163E-4	0.0	0.0	28.460045963435057	0.0
33	1.491350910395163E-4	0.0	0.0	28.892537727449657	0.0
34	1.491350910395163E-4	0.0	0.0	29.482814417784063	0.0
35	1.491350910395163E-4	0.0	0.0	29.940510012184337	0.0
36	1.491350910395163E-4	0.0	0.0	30.397012525856297	0.0
37	1.491350910395163E-4	0.0	0.0	30.823240616047233	0.0
38	1.491350910395163E-4	0.0	0.0	31.252451408058963	0.0
39	1.491350910395163E-4	0.0	0.0	31.707611705911567	0.0
40	1.491350910395163E-4	0.0	0.0	32.19662566943014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAACG	35	1.018725E-7	46.000004	45
CTCACGT	30	1.860235E-6	46.0	44
ATAGCGG	25	3.415622E-5	46.0	1
ATTAACG	20	6.309564E-4	46.0	10
AGGGTTG	60	0.0	46.0	5
ACGGGTA	20	6.309564E-4	46.0	4
CTAACGG	85	0.0	46.0	1
CGAGGGT	20	6.309564E-4	46.0	3
AGATTGG	80	0.0	46.0	1
AACGAGT	20	6.309564E-4	46.0	29
GTCCCGT	20	6.309564E-4	46.0	17
TATCGGC	20	6.309564E-4	46.0	12
ATCGAGG	80	0.0	46.0	1
TCTATCC	25	3.415622E-5	46.0	22
CCGCAAT	55	1.8189894E-12	45.999996	32
ACGGGAT	290	0.0	45.206898	4
TTACGGG	185	0.0	44.756756	2
CGTTTTT	1105	0.0	44.126694	1
CACGAGG	110	0.0	43.90909	1
CGGGATA	105	0.0	43.809525	5
>>END_MODULE